Vol. 32, March, 2010

scary movie scene du viagra 1/32 COMPARISON BETWEEN HYDROXYAPETITE COATED AND ROUGH-BLASTED MODULAR FEMORAL STEMS IN REVISION TOTAL HIP REPLACEMENT

Yossry M. Mousa*, Ibrahim M. Ganzoury* and Mohamed A. Noaman**

*Department of Orthopaedics and Trauma, Ain Shams Univerisity Hospital,

El Abasia, Cairo, Egypt.

**Department of Orthopaedics and Trauma, Nasr city Insurance Hospital, Yossef Abbas Street, Nasr City, Cairo, Egypt.

Revision total hip arthroplasty is becoming a more demanding and difficult procedure. Removal of a prosthesis stem in hip revision arthroplasties, severe femoral osteolysis or thick femoral cement mantles can cause intraoperative femoral defects or fractures that need to be bridged by long revision stems. A full coating of hydroxyapatite might increase osteointegration which lead to ongrowth over the whole intramedullary surface. Twenty revision total hip arthroplasties were performed, 10 of 20 hips using a modular stem coated by hydroxyapetite, whereas the remaining 10 hips using a modular stems with rough-blasted surface. Femoral stability of the 2 implant were compared at 2 years follow-up. Neither difference in the mean postoperative Harris hip score among the patients of both groups, nor in result or complication between the two groups. Bone fixation was achieved more often with cementless modular long-stem system, but no significant difference was noted in outcomes between the two stems.

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vente viagra sans ordonnance belgique 2/32 PREPARATION AND EVALUATION OF NAPROXEN GELS

Shereen A. El-Adawy

Pharmaceutical Department, Faculty of Pharmacy (girls), Al-Azhar University, Nasr City, Cairo Egypt.

Gels dosage forms are successfully used as drug delivery systems considering their ability to control drug release and to protect medicaments from hostile environment. Thus, it was desired in this study to formulate naproxen into different gel bases. Different topical formulations gel bases of naproxen of 1% concentration were prepared and evaluated, like Carboxymethylcellulose (CMC), Hydroxypropylmethylcellulose (HPMC), Carbopol 934 and pectin gels. Different formulae were characterized in terms of drug release, spreadability, rheological properties of plain and medicated gel bases using Brookfield viscometer. From rheological data, the yield values, thixotropic behavior and the Farrow il viagra migliora le prestazioni sessuali ,s constans (N) were calculated. The formulae showing the best drug release were selected for clinical study of the anti-inflammatory effect of the drug in topical gel bases by measuring the mean percentage inhibition in edema thickness in rats   related to control group. Pectin 4%gel base, followed by 4%HPMC showed the highest amount of naproxen release and used for clinical study of the anti-inflammatory effect of the drug in gel bases by measuring the mean percentage of inhibition in edema thickness in rats and it was compared to control group and received Naproxen oral suspension. Finally from this study it was possible to formulate naproxen gel delivery system which can produce significant topical anti –Inflammatory effect

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sildenafil citrate cas 3/32 PHARMACODYNAMIC EVALUATION OF CLARITHROMYCIN and LEVOFLOXACIN IN COMMUNITY-ACQUIRED PNEUMONIA USING MONTE CARLO SIMULATION

Mokhtar M. Mabrouk, *Wageh M. Awara, **Sahar M.El-Haggar

and **Ahmed A. El-Sherbeni

Department of Analytical Chemistry, *Department of Pharmacology, **Department of Clinical Pharmacy, Faculty of Pharmacy, Tanta University, Egypt

The purpose of this research was to perform a pharmacokinetic pharmacodynamic (PK-PD) target attainment analysis to compare the pharmacodynamics of clarithromycin and levofloxacin in the Egyptian adult populations. Forty eight volunteers received a single oral dose (500 mg) tablet of either levofloxacin or clarithromycin where pharmacokinetic parameters were determined by non-compartmental methods. Monte Carlo simulations (10 000-subjects) were performed to calculate the probability of target attainment (PTA) and the cumulative fractions of response (CFR) for blog cialis generique Streptococcus pneumonia and viagra super active plus australia Haemophilus influenza pathogens using regional minimum inhibitory concentration (MIC) distribution data. The pharmacodynamic targets was the area under the free concentration-time curve over 24 hours (fAUC0-24) to MIC of 35, 125 and 30 for clarithromycin and levofloxacin against gram-negative and against S. pneumoniae, respectively. The results demonstrated that (mean ± SD): AUC0–T, Cmax, Tmax, half-life, apparent systemic clearance of clarithromycin were 15.68 ± 4.39 mg.hr/L, 3.85 ± 1.58 mg/L, 2.48 ± 0.31 hr, 3.49 ± 1.1 hr, and 34.92 ± 12 L/hr, respectively. While for levofloxacin were 47.48 ± 10.34 mg.hr/L, 4.76 ± 1.25 mg/L, 1.6 ± 0.89 hr, 8.93 ± 3.57 hr, and 11 ± 2.35 L/hr, respectively. The CFR was >90% for the two antibiotics against both pathogens in epithelial lining fluid (ELF). CFR in the serum was 79.3% and >90% for levofloxacin and 73% and 0.2% for clarithromycin against S. pneumoniae and H. influenza respectively. In conclusion, levofloxacin resulted in a higher CFR in both serum and ELF than clarithromycin during the treatment of both pathogens. Clarithromycin at conventional doses is also considered optimum to treat community-acquired pneumonia caused by acheter viagra feminin S. pneumoniae and H. influenzae regarding its CFR in ELF (>90%).

 

 

4/32 INFLUENCE OF BEVERAGES AND MEALS ON THE ORAL BIOAVAILABILITY OF CIPROFLOXACIN AS A MODEL CLASS III DRUG

Hanaa A. Elghamry1, Mohsen A. Hedaya 2,3, Sahar M. El-Haggar2

and Osama A. Fahmy1

1Department of Pharmaceutical Technology, Faculty of Pharmacy, Zagazig University, Egypt

2Department of Clinical pharmacy, Faculty of Pharmacy, Tanta University, Egypt

3Department of Pharmaceutics, Faculty of Pharmacy, Kuwait University, Kuwait

The purpose of this research was to investigate the effects of beverages and meal composition on the oral bioavailability of ciprofloxacin in normal healthy volunteers in two separate single dose crossover pharmacokinetic studies. Ciprofloxacin is a synthetic broad spectrum antimicrobial agent which is categorized as a class III drug according to the FDA's Biopharmaceutics Classification System (BCS). In the first study, ten fasted healthy volunteers received 500 mg of ciprofloxacin with 200 ml of water, acidic beverage, and alkaline beverage in a three-way crossover study with one week washout period between treatments. In the second study, ten healthy volunteers received 500 mg of ciprofloxacin with 200 ml of water after an overnight fast, after eating a standardized, fat-rich, and protein-rich meals in four-way crossover study with one week washout period between treatments. The results demonstrated that the acidic beverage significantly (P<0.05) decreased the bioavailability of ciprofloxacin by 25% compared to water and by 34% compared to alkaline beverage as indicated by the reduction in ciprofloxacin AUC. This can be explained by a reduction in the dissolution rate of ciprofloxacin tablets due to the transient decreased in the gastric pH after the acidic beverage. All types of meals delayed the rate of absorption of ciprofloxacin as indicated by the significant prolongation of the time to achieve maximum drug concentration. The fat-rich meal significantly (P<0.05) reduced the bioavailability of ciprofloxacin by about 38% compared to fasting state. These results may be attributed to the delay in gastric emptying, reduced ciprofloxacin dissolution, and possible inhibition of ciprofloxacin absorptive transporter by the fat-rich meal. The obtained results are in agreement with the expected food effect on class III drugs according to BCS. These findings indicate that beverages and meals of different compositions have variable effects on the bioavailability of ciprofloxacin.

 

 

5/32 PHENOLICS FROM BLUEBIRD VINE(PETREA VOLUBILIS JACQ) CULTIVATED IN EGYPT

Atef A. El – Hela, *Hussein Al-Amier and *Ibrahim Al-Ghazaly

Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.

*Horticulturre Deparrtment, Faculty of Agrriculturre, Al-Azhar Univerrrsity, Nasr City, Cairo, Egypt.

From leaves of Petrea volubiils Jack (Bluebird Vine) cultivated in Egypt, phenolic acids, caffeic, ferulic, syringic, P- hydroxybenzoic, chlorogenic and protocatcheuic acid and flavonoids , apigenin, isooriantin, vitexin, luteolin 7-O-glucoside and isovitexin in addition to other compounds benzoic –O β-D- xylopyinosoyl- 1- 3 – O – α. L rhamnopyronosyl – (1-4) –O- β-D- glucopyranoside), benzoic –O β-D - pyronosyl - 1- 3 – O β-D – pyronosyl, decane 4,6 diyene -1-β- glucopyranosyl and Eukovoside. The structures of all isolated phenolic acids and flavonoids compounds were achieved by chromatographic and spectroscopic analysis (TLC, PC, HPLC and UV), while the other compounds were identified by classical spectroscopic (UV, IR , 1HNMR and 13CNMR) methods. Quantitative estimation of the total phenolic (500 µg/gm Dw), flavonoid (88.7 µg/gm Dw), and phenylpropanoid glycoside (218.8 µg/gm Dw) content of the methanolic extract of Petrea volubiils Jacq herb cultivated in Egypt was done using folin-Ciocalteu reagent, aluminium chloride reagent and Arnow,s reagent respectively, whereas the antioxidant effect of the methanolic extract was estimated by the stable free radical 1, 1-diphenyl -2- picrylhydrazyl (DPPH) method. The tested extract showed significant relation between the free radical scavenging potency and the total phenolic, flavonoids and phenylpropanoid glycoside content of the extract..

 

6/32 SYNTHESIS AND ANTITUMOR ACTIVITY OF SMALL ORGANIC MOLECULES BASED ON FUSED THIOPHENE SCAFFOLD

Yasmine Mohamad,Mohamed M. Said, *Adel A. El-Gendy and

**Khaled A.M. Abouzid

Organic Chemistry Department, Faculty of Pharmacy, Suez Canal University and *Misr International University, Egypt.

**Pharmaceutical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt

A series of novel tetrahydrobenzo[b]thiophene and their oxygenated analogues , pyranothiophene, were synthesized and tested for their biological evaluation as anticancer agents on the human colorectal carcinoma cell line. Most of the tested compounds exploited potent antitumor activity with IC50 values in the nanomolar range in particular compound VIIb, which displayed the highest activity among the tested compounds with IC50, equal to 21.28 nmol.

 

7/32 ENVIRONEMENT AS WELL AS HEALTH CARE WORKERS AS SOURCES FOR POTENTIAL NOSOCOMIAL PATHOGENS OF INTENSIVE CARE UNITS IN EGYPTIAN HOSPITAL AFFILIATED TO AL-AZHUR UNIVERSITY.

B) NEONATAL INTENSIVE CARE UNIT IN AL-HUSSEIN UNIVERSITY HOSPITAL.

Mahmoud M.T. Abd Raboh, Tarek R. Mohamed and Al-Said A-H.M. Helal

Microbiology and Immunology Department, Faculty of Pharmacy, Al-Azhur University, Cairo, Egypt

Neonates admitted to intensive care units (NICUs) are usually at higher risk of infection, since their immune system is not well developed. Nosocomial infections (NIs) may originate from either endogenous or exogenous sources as animate or inanimate sources within hospital. This study is devoted to investigate potential nosocomial pathogens in these exogenous sources in NICU at Al-Hussain University Hospital. Ninety three isolates from neonatal intensive care unit (NICU) at Al-Hussein University hospital were identified and tested for antimicrobial sensitivity. Seventy seven isolates (82.8%) were Gram positive, whereas sixteen isolates (17.2%) were Gram negative. In a descending order the frequencies of Gram +ve isolates were CNS 43%> Bacillus spp 29%> Micrococcus spp. 6.45%> S.aureus 4.3%. Klebsiella spp was the most frequent isolate followed by Serratia Spp and E.coli.Statistical analyses showed the environmental origin of bacillus spp., Serratia spp., whereas E.coli and Klebsiella spp. were detected in incubator area referring to patient as major source. S.aureus was detected only on hands of health care workers (HCW) and at incubator. Finally, CNS pattern of distribution was of complex nature with significant differences between antimicrobial patterns of CNS isolates of HCWs and those of environment. Antimicrobial susceptibility patterns of CNS, Bacillus, and Gram –ve isolates were of hospital origin, whereas S.aureus isolates were of community origin. The implications of these results are discussed in the text.

 

8/32 EFFECT OF GINGER ON THYROID FUNCTION AND TESTOSTERONE IN MALE RATS

Haleema Yousef Al-Nahari

Department of Biological Sciences, Faculty of Science, King Abdul Aziz University, Girls Sciences College. Saudi Arabia

Ginger is important cooking spice flavor food and herbal medicine. It has strong anti-oxidant properties. The aim of the study is to investigate the effects of daily injection of ginger Zingiber officinale on thyroid function, testosterone, cholesterol and testes weight in adult male rats. Adult male rats were divided into two main groups, group (1) as control; which were daily injected with saline solution for four weeks, group (2) received a 500 mg/kg/day of ginger Zingiber officinale interperitoneal (ip) injection for four weeks (5) rat from group 1and 2 killed every week. The study included measuring the level of thyroid stimulating hormone (TSH), triiodothironine (T3), thyroxin (T4), the ratio of triiodothironine to thyroxin, testosterone, cholesterol and testis weight. Aqueous extract of ginger Zingiber officinale at 500 mg/kg/day had recorded significant increase in thyroid stimulating hormone (TSH) after forth week, triiodothironine (T3) after second and third week, thyroxin after second week , triiodothironine to thyroxin ratio third week, and increase in testosterone after third week, significant increase in cholesterol after first and third weeks. And significant increase in testes weight after second week. The data suggest that ginger has both positive effect of antioxidants and potent androgenic properties on thyroid gland and testis

 

9/32 INFLUENCE OF GINGER ON THYROID FUNCTION AND TESTIS IN CARBONTETRACHLORIDE- INDUCED HEPATOTOXICITY RATS

Haleema Yousef Al-Nahari

Department of Biological Sciences, Faculty of Science, King Abdul Aziz University,                 Girls Sciences College, Saudi Arabia

Ginger root Zingiber officinale has been used for thousands of years in the far east. It is an important cooking flavor food and herbal medicine around the world, the ginger family (Zingiberaceae). The aim of the study was to investigate the protective effect of ginger Zingiber officinale on thyroid function and testis in carbontetrachloride induced hepatotoxicity in rats. Adult males albino rats divided into four main groups, first group as control and received saline solution i.p. for four weeks , second group received carbon tetrachloride CCl4 0.1ml/150 g b.wt for one week, third group received aqueous extract of ginger Zingiber officinale i.p. 500 mg/kg body weight / day for three weeks and then received carbon tetrachloride CCl4 0.1ml/150 g b.wt for one week with ginger, fourth group received a 500 mg/kg body weight / day of aqueous extract of ginger Zingiber officinale i.p. for three weeks and then received CCl4 for one week . The study includes measuring the level of thyroid stimulating hormone (TSH), triiodothironine (T3), thyroxin (T4), the ratio of triiodothironine to thyroxin, testosterone, cholesterol and testes weight. The result show significant decrease in thyroid stimulating hormone (TSH), triiodothironine (T3), thyroxin, testosterone and testes weight in rat treated with CCl4 (group2) compared with control (group 1). While there were increase in cholesterol in rat treated with CCl4 (group2) compared with control (group 1). The present results recorded significant increased in TSH,T3 and testosterone in group 3 ( ginger and CCl4) and group4 (treated with CCl4 after ginger ) compared with group 2(treated with CCl4) .There was significant increase in weight of testis in group 4 compared with group 2. thus suggesting that ginger has anti-oxidant effects and therefore provides protective effect on several body organs and tissues including thyroid glands and testis.

 

10/32 DIETARY THERAPY FOR CHOLESTROLEMIA MAY SUPPORT LIVER FUNCTION. A RAT MODEL STUDY

Ali I.S. Ahmed, Amira A. Shetewy*, Ahmed M. Gaafer and Khaled M. Nematalla

Special Food and Nutrition, FTRI, ARC, Giza and * Fac. Special Edu. Alex Univ. Egypt.

The liver of hypercholesterolemic aged rats was examined with dietary protein either as rich protein antioxidant formula (ACES) or by injection with a unique patent protein (PPD). The hypocholesterolemic promotion with vitamin E and Selenium (ES) in soy, sunflower and flax mixture was important. The addition of Ascorbic acid and Carotenoides (AC), especially from the extract of parsley curly (P), to make ACES formula, was more effective to support liver homeostatic role. This has been demonstrated in terms of high correlation between liver function and organ pathology. Soyprotein had less metabolic capability in the process of liver protection in absence of the antioxidant (AO) due to a lack in cholesterol distribution upon high fat ingestion. It can play a role in some AO distributions or may affect AO intake and modulates hepatic contribution to systemic inflammation. Moreover, the trafficking function of these proteins may consider an attractive description to this phenomenon. It may apply micronutrients in such a way that they can selectively reach the target tissue. Therefore, pure biological role for structural plant proteins PPD was estimated. The biological protection here can be evaluated on principles of nanotechnology. Actually, PPD may facilitate a hormonal balance enhances the whole biological system with respective micronutrient. Thus, the ACES supplementation or PPD injection, in different multimechanisms, protected rats aganist hepatotoxicity that generated by hypercholesterolemia.

 

 

11/32 CHANGES OF HEMOGLOBIN STRUCTURE BEFORE AND DURING CANCER BREAST RADIOTHERAPY

Zinab H. El-Tanahy*, Sozan N. El-Sayed*, Aisha S. El-Din **, Samir A. Nouh ***, Laila Anis **** and Hoda A. Farooh ****

*Physics Dept., Faculty of Sciences, Al -Azhar University (Girls); ** Biophysics Dept. NCRRT; *** Physics Dept., Faculty of Sciences, Ain Shams University and **** Health Rad. Res. Dept., NCRRT.

The structural modifications in hemoglobin samples, taken from healthy donors (negative control) and from patients receiving radiotherapy before and during their treatments, have been studied as a function of dose using different characterization techniques such as X-ray diffraction (XRD), viscosity, Fourier Transform Infrared spectroscopy (FTIR), electron spin resonance (ESR), and UV-Visible spectroscopy. Cancer patients showed many different changes in the physical properties of their hemoglobin. The results of integral intensity measured by X-ray diffraction was decreased compared to the –ve control, which indicates a decrease in the crystalline phase (ordering). The hemoglobin viscosity increased in all cases. Also, the FTIR Hb., spectra indicate that breast cancer patients suffered from destruction in all amide groups. The ESR indicated an increase in the number of free radicals. The UV-Visible Hb., spectra showed stretching in globin–heme interaction band. On radiotherapy of cancer patients the integral intensity measured by X-ray diffraction , the hemoglobin viscosity, the intensities of amide groups and the number of free radicals were increased.

 

 

12/32 COMPARATIVE BIOAVAILABILITY STUDY OF CEFUROXIME AXETIL TABLETS

Hammam A. Mowafy, *Yousif A. Asiri, **Adnan A. Kadi, *Badraddin M. Al-Hadiya, *Khalil I. Al-Khamis and Yousry M. El-Sayed

Department of Pharmaceutics, College of Pharmacy,*Department of Clinical Pharmacy and 3Department of Pharmaceutical Chemistry, King Saud University, Riyadh, Saudi Arabia.

This study was performed to investigate the bioequivalence of cefuroxime axetil tablets between a generic Test Product (A) Zednad® Tablet (500 mg cefuroxime/tablet, Diamond Pharma, Syria), and the Reference Product (B) Zinnat® Tablet (500 mg cefuroxime/tablet, GlaxoSmithKline, Saudi Arabia). The bioavailability study was carried out for 24 healthy male volunteers. The subjects received one Zednad® Tablet (500 mg/tablet) and one Zinnat® Tablet (500 mg/tablet) in a randomised, two-way crossover design fashion on 2 treatment days, after an overnight fast of at least 10 hours, with a washout period of 7 days. Twenty-four (24) volunteers plus two alternatives completed the crossover. The bioanalysis of clinical plasma samples was accomplished by HPLC method, which was developed and validated in accordance with International guidelines. Pharmacokinetic parameters, determined by standard non-compartmental methods, and ANOVA statistics were calculated using SAS Statistical Software. The significance of a sequence effect was tested using the subjects nested in sequence as the error term. The 90% confidence intervals for the ratio between the test and reference product pharmacokinetic parameters of AUC0®t, AUC0®¥, and Cmax were calculated and found to be within the confidence limits of 80.00—125.00% for AUC0®t, AUC0®¥ and Cmax. The study demonstrated that the test product (A) was found to be bioequivalent to the reference product (B) following an oral dose of 500 mg tablet. Therefore, the two formulations were considered to be bioequivalent.

 


13/32 SODIUM CARBOXYMETHYL CELLULOSE CONJUGATES FOR MUCOSAL DRUG DELIVERY

Doaa H. Shakshak, Noha M. Zaki, Nahed D. Mortada and Abdel Hamid ElShamy

Department of Pharmaceutics, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Abbassia, Cairo, Egypt

The development of bioadhesive drug delivery systems allows for therapeutic levels of bioactive to be maintained locally, extends its residence time within the body and reduces its dosing frequency and quantity administered. The aim of this study is to enhance the bioadhesion of sodium carboxymethyl cellulose (CMC) polymer as a carrier for mucosal drug delivery. To fulfill this aim, chemical modification of CMCwas done by covalent conjugation to L-cysteinevia carbodiimide chemistry. The reaction was done at different pHs followed by determination of the thiol content, the swelling behavior as well as the bioadhesive force of modified polymer as compared to the unmodified one CMC. Results revealed that the modification resulted in increased mucosal adherence but did not markedly affect the swelling behavior. On the other hand, FTIR and HNMR spectroscopy revealed the formation of sulfhydryl-containing CMC with superior interaction with mucin lining the mucosal membranes. These results represent the potential of CMC modified with cysteine in improving the mucoadhesive properties of the former for mucosal administration.

 

 

14/32 LEPTIN (L), ADIPONECTIN (A) SERUM LEVELS AND LEPTIN/ADIPONECTIN (L/A) RATIO ARE PLAUSIBLE BIOMARKERS OF BREAST CANCER IN OBESE POSTMENOPAUSAL EGYPTIAN WOMEN

Mohamed A. El-Desouky,Fathy M. Abdelgaleel, Fatma El-Zahraa M. El-Husseini, and *Zeinab M. Abdel Hafeez

Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.

*Oncology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Breast cancer in Egypt is the most prevalent cancer among women. Obesity is a known risk factor for breast cancer and it increases breast cancer risk by 30%. The exact mechanism has not yet fully elucidated but it has been assumed that there are different biologically active factors that are secreted by adipose tissue, such as estrogens, insulin-like growth factors and adipocytokines as “Leptin and Adiponectin” are found to be related to some cancer risks and tumor biology. The aim of this work was to evaluate the two adipocytokines: Leptin (L) and Adiponectin (A) as well as their ratio serum levels (L/A ratio) in addition to insulin in women suffering from benign breast diseases and in those with breast cancer with different stages and compare them to those in normal healthy postmenopausal ones. Fasting Leptin, Adiponectin and Insulin serum levels were measured in all samples using Enzyme-Linked ImmunoSorbent Assay (ELISA). All groups were of matched age and BMI. Serum leptin (L) levels were found to be elevated in breast cancer patients suggesting that leptin may act as a new biomarker in breast carcinogenesis. Serum adiponectin levels were on the contrary of those reported on leptin levels to be significantly lower and in a decreasing order in the different stages of breast cancer patients than those in the corresponding values in age- and BMI-matched control ones. The decrements were in parallel with the increase in tumor size and the presence of lymph node metastasis. So, low serum adiponectin levels may be of novel risk factor for breast cancer and may serve also as a new biomarker in breast carcinogenesis and might provide a new insight into the understanding of the association between obesity and breast cancer risk. L/A ratio levels are increased significantly in breast cancer patients with larger size of tumor and with positive lymph node metastasis in comparison to controls and this might allow its use as a biomarker of breast carcinogenesis in those obese postmenopausal Egyptian women. The serum levels of insulin were shown to be increased significantly and progressively in breast cancer patients with the different tumor stages in comparison to the controls. Serum insulin levels seemed to be affected by the tumor size and the presence or absence of lymph node metastasis. These results may indicate the role of high insulin serum levels in breast carcinogenesis and may refer to the crucial role of insulin as a risk factor of breast cancer.

 

15/32 PHENOLIC ACID CONTENTS FROM VERBENA RIGDA SPRENG AND POTENTIAL ANTIOXIDANT AND ANTI MICROBIAL ACTIVITY

Atef A. El-Hela, Al Sayed M. El- Ghaly and *Farag M. Shaieb

Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.

*Department Of Botany, Faculty of Science, Omar El Mokhtar University, Elbeda, Libya

Verbena rigdaSpreng. herb (Verbenaceae) was analyzed (PC,TLC and HPLC methods) for the presence of phenolic acids. This was the first study of the phenolic acid content of this species. Phenolic acids were prepared by the standared method and analysed by HPLC with UVdetection. Identification was performed by comparing retention times with those of standards. Quantitative determination was performed by colourimetric method and HPLC (absorbance maximum of phenolic acid at λ765 and 280 nm), which gave 49.4 and 72.8mg % respectively. As the result of our study, ten phenolic acids, both free and liberated by alkaline and acid hydrolysis, were identified by HPLC. Moreover HPLC was applied to the quantitative assay of major components of phenolic acid fraction. The concentration of o-coumaric acid, the major compound in free phenolic acid set is 57mg% and the amount of caffeic acid which dominates in the extracts liberated by acid and alkaline hydrolysis is 2.7 mg% and 2.4mg%, respectively.The antioxidant and antimicrobial activity of the phenolic acid fraction was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and total antioxidant capacity, the study showed that phenolic acid fraction has significant activity with 0.02 IC50(mg ml-1). The antibacterial activity was studied invitro on bacterial strains Staphylococcus aureus, Bacillus subtilius, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. In conclusion, the results indicate that the phenolic fraction have the capacity to scavenge free radicals, and to inhibit the growth of pathogenic microorganisms. Therefore, they could be suitable for using as antimicrobial and antioxidative agents in the food industry.

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