Vol. 42, July, 2013.

achat cialis en france 1/42 EFFECT OF SURFACTANT STRUCTURE AND PREPARATION TECHNIQUE ON THE PROPERTIES OF NIOSOMES ENCAPSULATING PIROXICAM–HP-Β-CYCLODEXTRIN COMPLEXES

Abdelaziz E. Abdelaziz

Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt

The combined approach of drug-cyclodextrin inclusion complex and its entrapment in niosomes has been recently investigated as a new potential strategy and merging the relative advantages of the two types of carriers into a single system. Equimolar inclusion complex of piroxicam and hydroxypropyl-β-cyclodextrin (HPβ-CD) was added at different concentrations to the aqueous phase of niosomes consisting of surfactant (span20, span 40, span 60 and span 80) and cholesterol (50%:50%). Niosomes were prepared by three techniques: lipid hydration method, sonication method and reverse phase evaporation method in order to obtain multi-lamellar vesicles (MLV), small uni-lamellar vesicles (SUV) and large uni-lamellar vesicles (LUV), respectively. Entrapment efficiency, size, morphology and in-vitro permeation studies of Piroxicam from the prepared niosomes were investigated. Niosome preparation method and structure of surfactant clearly affected both niosome size and drug loading capacity. Encapsulation efficiency increased in the following order: Span 60>Span 40>Span 20>Span 80. Also encapsulation efficiency increased with increasing the complex concentration up to 10 mM, and was in the order MLV>LUV> SUV. Permeability of piroxicam–HPβ-CD complexes, directly in solution or incorporated in niosomes was studied. As expected, a prolonged permeation of niosomal formulations. Furthermore, the drug permeation rate depended on the vesicle characteristics varied in the order: SUV>MLV>LUV. Cyclodextrin complexation improved drug solubilization and allowed a strong improvement of its entrapment into the aqueous niosomal phase and better controls the release of poorly soluble drugs (piroxicam).

commande viagra en ligne france 2/42 AGED GARLIC EXTRACT ADMINISTRATION PROTECTS RAT HEART FROM THE HAZARDS OF IONIZING RADIATION

Ehab T. Mohamed and Ahmed A. Elkady

Health Radiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.

The aim of this study was focused on investigating the possible protective effect of Aged garlic extract (AGE) against gamma radiation induced cardiotoxicity and inflammation in male albino rats. Twenty four albino rats were divided into four equal groups as follows: control group; without radiation and treatment, irradiated group; animals subjected to whole body gamma irradiation at a dose of 6 Gy, treated group; rats were given orally AGE at a dose of 250 mg/kg/day for 4 weeks, and treated irradiated group; animals were treated orally with AGE at a dose of 250 mg/kg/day for 4 weeks then exposed to whole body gamma irradiation at a dose of 6 Gy. The obtained results revealed that the administration of AGE to irradiated rats significantly ameliorated the changes occurred in the investigated biochemical parameters. The histopathological results showed distinctive pattern of myocardial injuries in irradiated group, while in treated-irradiated group the myocardial tissues showed relatively well-preserved architecture with or without congested blood vessels effet benefique du viagra . In conclusion, AGE acts as a potent scavenger of free radicals to prevent or ameliorates the toxic effects of gamma irradiation. Also, AGE might provide substantial protection against radiation-induced inflammatory damages.

prix reel du viagra 3/24 DESIGN, FORMULATION, IN-VITRO RELEASE AND STABILITY OF SILYMARIN USING DIFFERENT LIPOSOMAL COMPOSITIONS

Ahmed M. Samy, Alaa A. Kassem, Sherief Khalifa, Hany Mahmoud and

Mohammed Elmowafy

Department of Pharmaceutics and Ind. Pharmacy, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo, Egypt

Silymarin is a natural hepatoprotective agent of low bioavailability from oral products. The objective of the present work was to design and formulate Silymarin in four different liposomal formulations and then the in-vitro release and the stability study were carried out. The first is conventional formula consisting of hydrogenated soy phosphatidyl choline and cholesterol (2:1 molar ratio). Second, third and fourth are formulated by adding β-Sitosterol, β-D-glucoside (hepatic targeting sterol), PEGylated lipid and both respectively. All were prepared by thin film hydration method followed by extrusion. Decreasing number of extrusion times from ten to five had a significant positive effect on encapsulation efficiencies and negligible effect on particle size. FT-IR studies revealed a possibility of interaction between Silymarin and phospholipids. Stability studies showed that all formulations were stable against methanol. Storage within 120 days revealed that formulations had little particle growth.    

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prix plaquette viagra 4/42 THE EFFECT OF LASER-ACTIVATED GOLD NANOPARTICLES IN TREATING DMBA- INDUCED ORAL SQUAMOUS CELL CARCINOMA IN GOLDEN SYRIAN HAMSTERS

Heba S. Ramadan, Taha I. Hewala* and Amani Kazem**

Medical Biophysics Department, *Radiation Sciences Department and **Pathology Department, Medical Research Institute, Alexandria University, Egypt

Heat generation by gold nanoparticles (Au NPs) is an important physical property in which Au NPs absorb the incident photons and transfer heat to the surrounding matrix and thus nominates the use of Au NPs as photothermal agents in cancer treatment. The present work aimed to figure out the therapeutic effects of hyperthermia induced by laser-activated anti- epidermal growth factor receptor (EGFR) antibody conjugated with gold nanoparticles that were injected in hamster bearing chemically- induced oral squamous cell carcinoma (OSCC). After developing cancer, animals were injected IV with gold nanosuspension (dimater =10 nm) and exposed to visible diode laser at 532 nm. The therapeutic effect of this treatment was evaluated by measuring differences in the tumor size and tumor temperature before and after treatment. In addition to histopathological examination of tumor tissues, the levels of serum malondialdehyde (MDA) and soluble Fas receptor levels were quantified before and after treatment. Also, the possibility of toxic effects on liver tissues was studied.   The obtained data showed significantly increased concentrations of sFasR and MDA in the tumor-induced subgroup compared with control group, while their levels significantly decreased in the subgroup that injected with gold nanosuspension and received laser treatment with significant decreased tumor volume confirmed by histopathological resuts that indicated evidence of tumour regression and the nests of squamous cells showed signs of degeneration; dyshesiveness, clefting & acantholysis. Also, Frequent apoptotic figures are noted, but there were no necrosis. Kaplan–Meier survival analysis showed that the mean survival time of animals bearing OSCC prolonged after injection with anti-EGFR/gold conjugates nanoparticles followed by laser irradiation compared with the mean survival time in the untreated subgroup.   At the same time, evaluation of the possible cytotoxicity on liver tissues showed no morphological changes were seen in liver biopsies and no significant changes were seen in total antioxidant levels in liver tissues among groups. We can conclude that laser irradiation combined with gold nanoparticles may be a good therapeutic modality for OSCC in golden Syrian hamsters with no cytotoxic effects on liver tissues at the time of treatment.

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utilisation dapoxetine 5/42 ASSESSMENT OF ADIPOKINE LEVELS IN LIVER CIRRHOSIS DUE TO HBV AND HCV INFECTION

Eman M. Ibrahim, Ehab T. Mohamed and Ehab Sh. El-Esseily

Health Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA)

Hepatic cirrhosis is common histological finding in patients with chronic hepatitis B and C virus (HBV, HCV) infection. In this study we sought to determine whether serum levels of three adipokines (leptin, adiponectin and resistin) show any biochemical correlation with hepatic cirrhosis in patients with chronic HBV and HCV infection. In the study, we examined a total of 31 patients with chronic HBV and HCV infection (19 males and 12 females) and 25 healthy control subjects (10 males and 15 females). Liver cirrhosis was scored on biopsies. Serum levels of TNF-α, leptin, adiponectin and resistin were determined by ELISA. The results showed that serum levels of TNF-α, leptin, resistin, and adiponectin were significantly higher in post-hepatitis B and post-hepatitis C cirrhotic patients compared to control group. In conclusion,patients with liver cirrhosis due to HBV and HCV infection showed significant increase in the studied adipokines hormones and the increments correlate with the severity of cirrhosis.

dependance au levitra 6/42 THERAPEUTIC ROLE OF ADDING OLIVE LEAF TEA TO THE TRIPLETHERAPY OF H. PYLORI

Nada F. Elnaidany and *Elsayd A. Abdelaal

Clinical Pharmacy Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA),

*Department of Hepatology National Liver Institute, Monofiya University, Egypt.

Standard triple therapy of H.pylori gives incomplete eradication especially in developing countries where poor sanitation, crowdedness and uncontrolled use of antibiotics are common. H. pylori infection causes inflammation and oxidative stress in gastric mucosa. Olive leaves have anti-microbial, anti-inflammatory and anti-oxidant. The present study aimed to test whether addition of olive leaf tea to triple therapy including amoxicillin, proton pump inhibitors (omeprazol) and metronidazol increase H. pylori eradication or not. 120 patients with H. pylori positive test were randomized into two groups, group A, patients (n = 90) were given standard triple therapy, including omeprazole 20 mg twice daily, amoxicillin 1000 mg twice daily and metronidazole 500 mg three times daily for 14 days beside olive leaf powder tea (Prepared by pouring a 200 ml of boiling water over a packet containing 5 grams powder of olive leaf and drinking three times daily before meals for 30 days). Group B, patients (n = 30) were given standard triple therapy including omeprazole 20 mg twice daily, amoxicillin 1000 mg twice daily and metronidazole 500 mg three times daily for 14 days. The success of H. pylori eradication was defined as a negative H. pylori antigen stool test 6 weeks after the completion of therapy. The results, after completion of the regimens, were H. pylori eradication in 81 out of the 90 patients (90%) in group A and 13 out of the 30 Patients (43.33%) in group B. Eradication rates were higher in group A than group B and were not statically different between men and women in the same group (p>.0.05). Addition of olive leaf tea to triple therapy increases the eradication rate of H. pylori.

7/42 THE APPLICATION OF CENTRAL COMPOSITE DESIGN FOR OPTIMIZATION OF KETOPROFEN NIOSOMES

Abdelaziz E. Abdelaziz, Ahmed M. Samy, Elsherbiny A. Elabd, Mohamed A. Raslan

Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy,

Al-Azhar University, Nasr City, Cairo, Egypt.

The objective of the present study was to investigate the effect of important formulation variables on drug entrapment in and drug release from niosome formulations of ketoprofen to obtain an optimized formula of Ketoprofen niosomes using central composite design. Contour and response surface plots were depicted based on the equation given by the model of the formY=b0+b1X1+b2X2+b3X3+b12X11+b22X22+b32X33+b12X1X2+b13X1X3+ b23X2X3+ b123X1X2X3 where Y is the measured response associated with each factor level combination. Niosomes were prepared by a lipid hydration method usingcentral composite design with three different variables include; Surfactant cholesterol ratio (X1), HLB (X2) and total lipid concentration (X3). The optimization procedure generated the maximum overall desirability value.Central composite design succeeded in optimization of the formulation ingredients on the entrapment efficiency and in vitro release of Ketoprofen niosomes. Response surface methodology gave a mean to understand the effect of variables for the development of Ketoprofen niosomes. Finally the optimization process provides a formula having optimum level of factors as 0.66:1 from X1, 7.86 from X2, and 34.18 from X3. This optimized formula produces entrapment efficiency (Y1)equal to 42.22 % and release after 1 h (Y2), 6 h (Y3), and 12 h (Y4), 28.89 %, 71.64 % and 91.31 % respectively and these observed values of the optimized formula were close to the predicted values. Our study proved that experimental design methodology could efficiently be applied for characterization and optimization of formulation parameters affecting entrapment efficiency and drug release from ketoprofen niosomes.

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