Vol. 44, March, 2014.

ou acheter du levitra en france 1/44 SIGNIFICANCE OF DICKKOPF-1 (DKK-1) AS A BIOMARKER IN CHRONIC HEPATITIS C AND ITS PROGRESSION TO HEPATOCELLULAR CARCINOMA

viagra ou cialis efeitos colaterais BY

Mohamed Abdel-Hamid Ahmed, Iman Hassan*,

Noha Hassuna and Alshaymaa Darwish**

cialis 20 mg effets secondaires FROM

Departments of Microbiology, Faculty of Medicine, Minia University

*Biochemistry, Faculty of Pharmacy for Girls, Al-Azhar University

and Matrouh Hospital of Fever**

Chronic hepatitis C virus (HCV) is the main cause of liver cirrhosis and hepatocellular carcinoma (HCC) in Egypt.HCC is needed to be early and effectively diagnosed to have a good prognosis. We assessed whether measurement of Dickkopf-1 (DKK1) and Alpha fucosidase (AFU) in serum could improve diagnostic accuracy for HCC related to HCV. The patients were recruited from National Liver Institute and National Cancer Institute Egypt. The patients were divided into three groups (HCC group, chronic HCV group and control group). Detection of serum levels of alpha feto protein (AFP) and DKK-1 by ELISA and AFU by colorimetric were done to all subjects. There was no significant difference between DKK-1 serum levels in HCC group and chronic HCV group (p-value>0.05) but there was a significant decrease between DKK-1 serum levels in HCC group and the control group (p-value<0.05). There was also a significant increase in AFU serum levels between HCC group and control group (p-value<0.0001) and between HCC group and chronic HCV group (p-value<0.05). In addition there were no significant correlations between serum levels of DKK-1, AFP and AFU (P-value >0.05). DKK-1 and AFU are good serum biomarkers for HCC. However, there was no evidence that DKK-1 is a biomarker for progression of chronic HCV to HCC.

 

2/44 PROTECTIVE EFFECT OF GINGER (ZINGIBIER OFFICINALE) FAMILY: (ZINGIBEROCEAE) AGAINST SOME TOXICANTS INDUCED LIVER DISORDERS IN ALBINO RATS

BY

Mohamed Fares, Zaky Tawfek, Mahmud M. Salem, Ahmed Mansour*

and Walid Abu-Shoaier­

duree efficacité cialis FROM

Department of Zoology, Faculty of Science, Al-Azahr University, Cairo, Egypt.

*Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azahr University, Cairo, Egypt.

The effect of ginger diet ( cialis 5 mg contre indication Zingiber officinale) upon hepatotoxicity induced in albino rats by chronic dose of CCl4 was studied. Animals were divided into five groups. The first group was control. The second group was feed with ginger diet (5%) for six weeks. The third group was injected with chronic dose of CCl4 (2 ml/kg subcutaneously) dissolved in corn oil (1:1) twice weekly for eight weeks. The fourth group was treated with the same dose of CCl4 and ginger in a combination for eight weeks. The Fifth group was treated with the same dose of CCl4 for eight weeks then treated with the same dose of ginger diet for six weeks. The Injected animals with CCl4 induced various histological changes in the liver. These changes include congestion of blood vessels, leucocytic infiltration, cytoplasmic vacuolization of the hepatocytes and fatty infiltration. CCl4 caused significant elevation in serum ALT (alanine aminotransferase), AST (aspartate aminotransferase) enzymes and ALP (alkaline phosphates). It also caused significant decrease in TP (total protein), Alb (albumin) and Alb/Glob ratio and significant increase in Glob (globulin). Treating animals with CCl4 and ginger diet in combination led to no marked significant physiological or histological changes. Treating animals with CCl4 then ginger diet in group five gave significant histological improvement induced by ginger diet and significant decrease in ALT, AST, ALP and Glob levels and significant increase in TP, Alb and Alb/Glob ratio. The results of the present work indicated that ginger diet might has ameliorative, antifibrotic and hepatoprotective effects in liver chronic toxification induced by CCl4. This effect may be due to its potent antioxidant and anti-inflammatory activities. But no marked hepatoprotective effect for ginger in combination with CCl4 due to the effect of antagonism.

comment utiliser le viagra 3/44 EVALUATION OF POSTANTIBIOTIC EFFECT OF NIOSOME ENCAPSULATED CIPROFLOXACIN AND CIPROFLOXACIN-ANTIBIOTIC COMBINATIONS USING SIMULATED IN VIVO MODEL

mode d'action du viagra BY

Abd El Ghany El-Khouly, Nadia El-Guink, Hamida Abou Shleib and

Moustafa El-Ammori

comment bien prendre viagra FROM

Department of Pharmaceutical Microbiology, Faculty of Pharmacy,

Alexandria University, Egypt.

The use of niosomes has been proposed as a potential way to increase the therapeutic efficacy of antibiotics. Bactericidal activity and postantibiotic effect (PAE) of niosome encapsulated ciprofloxacin, free ciprofloxacin (CIP) and its combination with amikacin (AK), clarithromycin (CLR), or piperacillin (PRL) against various clinical isolates were studied using simulated in vivo model which mimics their serum profile in vivo. The test organisms were selected to represent variable antibiotic susceptibilities. The results obtained showed different bactericidal activities (Killing rate: K-1 and D value) and PAE determined by the time required to increase bacterial count by 10 times as well as the growth rate (K). All combination systems showed better activities than CIP alone. The best bactericidal and PAE occurred when the antibiotic treatment systems CIP/AK showed eradication of the organism as in case of the clinical isolates comment procurer du viagra Escherichia coli E1, viagra billig kaufen ohne rezept E4, Klebsiella pneumoniae K4 and Staphylococcus aureus S1, followed by systems possessing effective bactericidal activities and long PAE (> 6hr) as in CIP/AK with Pseudomonas aeruginosa, K. pneumoniae, Proteus mirabilis, S. aureus and Staphylococcus epidermidis isolates where K-1 varied between 2.2x10-3 and 6x10-2 and PAE values ranged from 4.6 to 25.1 hr. CIP/CIP combination showed good bactericidal activity K-1 from 2.7x10-3 to 4x10-2 and long PAE ranging from 2.2 to 12 hr followed by CIP/PRL showing PAE values between 2.7 and 9.2 hr and K-1 from 4.4x10-3 to 4.3x10-2 against all the tested organisms. CIP/CLR also exhibited good bactericidal activity K-1 ranging from 4x10-3 to 6.6x10-2 and PAE values from 1.8 to 9.2 hr against all the tested organisms, although CLR was inactive against the Gram-negative organisms. The least effective treatment was considered when there was no bactericidal effect and a very short PAE (< 30 min). In case of niosome encapsulated CIP, the released CIP showed a high and persistent bactericidal activity and a longer PAE against all tested organisms. The gradual release of CIP from CIP-niosomes produced a cumulative effect resulting in eradication of sensitive isolates E1, E4, K4 and S1, a long period of growth suppression (PAE) for moderately sensitive strains and a lower growth rate for resistant strains Ps. aeruginosa Ps12, Ps14 and P. mirabilis Pr2. The PAE produced with niosome encapsulated CIP was longer than that of the free CIP by 2-10 times (2.75- 26.6 hr). The present study demonstrated that entrapment of CIP into niosomes lead to prolongation of drug release and exhibited bactericidal activities and PAE values better than those of free CIP. Similar results were obtained with CIP/AK systems where eradication of sensitive strains was observed and marked bactericidal effect together with long PAE values were obtained with the other bacterial strains. However, the use of encapsulated CIP has the advantage over CIP/AK combination in prolonging the existence of CIP in systemic circulation thus enhancing penetration, and improving drug retention into the target tissue, and in turn increasing the therapeutic efficacy of the drug, reducing resistance development and toxicity problems.

4/44 MOLECULAR CHARACTERIZATION, ENZYME PROFILE AND SENSITIVITY TO ANTIFUNGAL AGENTS OF CANDIDA SPECIES INVOLVED IN VAGINAL INFECTION IN YEMEN

BY

Khalid Nasher Kahtan

FROM

Department of Biology/Chemistry, Faculty of Education, Radfan, Aden University, Yemen

In the present study 25 samples of vaginal swabs obtained from women complaining of vaginal candidiasis were collected. Samples were provided by Gynecologists in Ibb Hospitals, Yemen. Direct microscopy and culturing on Sabouraud dextrose agar revealed that all samples were positive for yeasts. Fungal strains were subcultured on Chromagar Candida for presumptive identification of species followed by molecular characterization based on rDNA sequencing. Seven Candida species were identified among which C. albicans was the most common being isolated from 56 % of patients. C. tropicalis and C. glabrata were associated with 16% and 12% of patients. C. dubleniensis, C. krusei, C. orthopsilosis and C. parapsilosis affected only 4% of cases. The majority of Candida strains were able to produce phospholipase, lipase, protease and phosphatase with varying capabilities. β- Haemolysis was detected in blood cultures of C. albicans (2 strains) and C. glabrata (1 strain). In-vitro sensitivity test showed that Nystatin exhibited a strong activity towards all Candida strains. Amphotericin-B was effective against most of the tested strains. Candida. dubleniensis, C. orthopsilosis and C. parapsilosis were sensitive to the different antifungal agents which included Amphotericin-B, Ketoconazole, Clotrimazole, Fluconazole, Voriconazole, Itraconazole and Nystatin.

Scroll to top