Vol. 47, July, 2015

1/47 STUDY THE RELATION OF25-HYDROXYVITAMIN D LEVEL ANDFATTY LIVERIN PATIENTSWITH TYPE 2 DIABETES MELLITUS

Gehan H.Ewieda,Eman M.I. Youssef, Mervat El- Shahat El-Wakeel *, Haneya A.A. Ali**andNashwa El-Khouly***

Department of Medical Biochemistry, Faculty of Medicine, Al-Azhar University (Girls)

*Department of Endocrinology, Faculty of Medicine, Al-Azhar University (Girls)

**Department of Microbiology & Immunology, Faculty of Medicine, Al-Azhar University (Girls)

***Department of Internal Medicine, Faculty of Medicine,Taibah University, KSA, Al-Azhar University (Girls)

Background: Vitamin D deficient individuals are more likely to develop alterations in glucose metabolism, such as impaired glucose tolerance, the metabolic syndrome and type 2 diabetes mellitus. Vitamin D is capable to reduce FFA-induced insulin resistance both in peripheral tissues and in hepatocytes. Therefore, low serum vitamin D may predispose to intrahepatic lipid accumulation leading to non-alcoholic fatty liver disease (NAFLD). Objective: This study aimed to determine the relation between the level of serum 25(OH) vitamin D and non-alcoholic fatty liver disease NAFLD in type 2 diabetes mellitus Subjects and Methods: Thecurrent study includedfiftydiabetic patients with NAFLDas group (I) and fortyhealthy subjects' sex and age matched to the patients as control group (II).Serum 25-hydroxyvitamin D level [25(OH)D)]using ELISA technique was done to all participants. Results: This study found that diabetic patients with NAFLD diagnosed by ultrasound examinationhad lowerserum level of 25(OH) vitamin D compared to control group with statistically significant difference between both groups. Conclusion: Diabetic patients with NAFLD had significantly decreased serum 25 (OH) D levels, suggesting that low 25(OH)Dstatus might play a role in the development and progression of NAFLD.

2/47 COMPARATIVE STUDY BETWEEN ALISKIREN, LISINOPRIL AND IRBESARTAN IN TREATMENT OF EXPERIMENTALLY- INDUCED METABOLIC SYNDROME IN RATS

Ahmed S. Mohamed, Mohamed E. Mansour, Ahmed Abu. El Gazar,

Omaima M. Abd Allah and Abeer A. Abd El Hameed

Department of Clinical Pharmacology, Benha Faculty of Medicine, Benha University.

Renin angiotensin system (RAS) is important for fluid and blood pressure regulation. Recent studies suggest that an overactive RAS is involved in the metabolic syndrome. The aim of this study was to examine the possible therapeutic effect of aliskiren (a renin inhibitor), lisinopril (ACEI) and irbesartan (ARB) in experimentally induced metabolic syndrome in rats and to compare between their effects. Thirty male albino rat were classified into five groups (n = 6 for each group): normal control group, metabolic syndrome- non treated group, metabolic syndrome was induced by ingestion of high fructose diet (60% fructose) for 8 weeks, metabolic syndrome induced group treated by aliskiren (100mg/kg/day) for 4weeks after induction of metabolic syndrome, metabolic syndrome induced group treated by lisinopril (10mg/kg/day) for 4weeks after induction of metabolic syndrome , metabolic syndrome induced group treated by irbesartan (30mg/kg/day) for 4weeks after induction of metabolic syndrome. The obtained results in the current work revealed that treatment of metabolic syndrome- induced rats by both aliskiren, lisinpril and irbesartan resulted in significant reduction of arterial blood pressure with more significant reduction in aliskiren treated group. Also the three drugs show improvement in hyperinsulinemia parameters with more significant improvement after aliskiren treatment. Aliskiren is more potent and more effective than lisinopril and irbesartan in treatment of metabolic syndrome.

 

3/47 THE POTENTIAL EFFECT OF VILDAGLIPTIN AND METFORMIN ON HEPATIC FUNCTIONS IN EXPERIMENTALLY INDUCED TYPE 11 DIABETES MELLITUS IN RATS

Mohamed E. Mansour, Mahmoud M. El-Fouly, Ahmed A.El Maaty El Gazar, Hanan T. Emam and Salwa A. Zeen Alabdeen

Department of Pharmacology and Therapeutics, Benha Faculty of Medicine,

Benha University.

The present study was designed to study the potential prophylactic effect of vildagliptin in diabetic non alcolic steatohepatitis (NASH) model of high fat and suger diet-streptozotocin (HFSD-STZ) induced type 2 diabetes mellitus (T2DM). 30 male adult rats were used. 1st group is control group (6 rats) was fed with standard diet. Experimental induction of HFSD-STZ induced T2DM, by feeding of rats with HFSD (83.25% basic feed + 5% sugar + 1.5% cholesterol) for 4wk combined with a single dose of STZ 40 mg/kg IP, rats which become diabetic with a glucose concentration >210 mg/dl would be used in the study then subdivided into 4 groups each group contains 6 rats then continue to be fed the same HFSD component for the next 8 wk in 12 wks study. Diabetic non rats will developed diabetic NASH at 6th wk of the study. 2nd groupdiabetic rats of in this group receive no treatment and were continuously fed with 8 weeks after development of DM.3rd group diabetic rats were given vildagliptin 15 mg/kg/day orally+ HFSD for 8weeks after development of DM. 4th group diabetic rats were given metformine 50 mg /kg/day orally +HFSDfor 8weeks after development DM. 5th group diabetic rats were given (vildagliptin + metformin) in combination with same previous doses+ HFSD for 8 weeks study after development DM.The obtained data in the current work revealed that oral monotherapy either with vildagliptin or metformin resulted in significant decrease (p<0.05) of fasting blood glucose level (FBG), serum alanine tranaminase (ALT), serum aspartate transaminase (AST) activity, serum cholesterol, serum triglycerides (TG), and serum low density lipoprotein cholesterol (LDL-C) with significant increase(p<0.05) of serum high density lipoprotein cholesterol( HDL-C) while co- administration of (vil+met) resulted in normalization of FBG level with significant increase of serum HDL-C level and with significant decrease (p<0.05) of other parameters compared to monotherapy groups and diabetic non treated group. It could be concluded that monotherapy with vildagliptin or metformin have partial hepatoprotective effect in experimental HFSD-STZ induced type 2 diabetic NASH, compared with diabetic non treated group while combination therapy (vildagliptin +metformin) treated diabetic group is the best group in this model of study and has hepatoprotective effect more than monotherapy as it is effective in improving liver function tests and improving total lipid profile.

 

4/47 THE POTENTIAL EFFECT OF ROSUVASTATIN ON NON ALCHOHOLIC STEATOHEPATITIS AND MYOCARDIAL INFARCTION INDUCED IN EXPERIMENTAL RATS

Rezk A. Sanad, Omaima M. Abdallah, Sherif A. Shaltout and Shaymaa H. Ismail

Department of Pharmacology and Therapeutics, Benha Faculty of Medicine,

Benha University

Rosuvastatin (Rsv) is one of statins, which are inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase which inhibit endogenous cholesterol synthesis, possess pleiotropic activities, such as anti-inflammatory, anti-oxidative and antifibrotic effects. Dyslipidemia is an important risk factor for acute coronary syndromes and non alcoholic steatohepatitis (NASH). The present study aimed to investigate the effect of Rsv on myocardial necrosis in an experimental model of acute myocardial infarction (AMI). Animals were given standarded chew supplemented with 2% cholesterol for 4 weeks then AMI was induced by isoprenaline injection (150mg/kg s.c), Rsv was given orally (1mg/kg/day) for 4 weeks before induction of AMI & intraperitoneally (i.p) (10 mg/kg) at the onset of AMI. The obtained result from the current work revealed that pretreatreament with Rsv significantly reduced serum lipid, serum CPK-MB, heart rate, T-wave voltage and myocardial necrosis and significantly increase serum HDL compared to infracted rats. This study also aimed to investigate whether Rsv ameliorate steatohepatitis using a high-fat and high-cholesterol diet-induced rat model. Animals were administered high fat and high-cholesterol diet via oral gavage for 6 weeks, Rsv was administered for 6 weeks (from the start of the study) and from the 6th week to the 10th week from the start of the study (after induction of NASH). The obtained results from the current work revealed that pretreatreament and treatment with Rsv produced significant decrease in serum lipid, ALT and AST ,significant increase in serum HDL and improvement of histopathlogical picture compared to hypercholesterolemic with no medication group. Conclusively, Rsv could havea cardioprotective effect against AMI & improved hepatic steatosis.

 

 

5/47 PREDICTING MEMBRANE PERMEABILITY USING IMMOBILIZED ARTIFICIAL MEMBRANE CHROMATOGRAPHY

Ahmed El Gendy and Adeboye Adejare*

Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy,

Al-Azhar University, Nasr City, Cairo, Egypt.

*Department of Pharmaceutical Sciences, Philadelphia College of Pharmacy, University of the Sciences in Philadelphia, Philadelphia, Pennsylvania 19104.

The partitioning of structurally diverse and functionally unrelated set of 21 compounds was studied on immobilized artificial membranes (IAM) column using high performance liquid chromatography (HPLC). The aqueous capacity factors (log KIAMW) of the compounds were determined and correlated with their permeability factor, the logarithms of octanol/water partition coefficient (log P), to drive whether molecular binding on IAM columns can predict permeability of drugs through biological membrane. Good correlation was obtained between log KIAMW and log P of the compounds (r = 0.952). For a subset of 8 compounds, log KIAMW showed better correlation with log % of human absorption than did log P. Though many in-vitro physicochemical based techniques are available to predict membranes permeability; data has shown that IAM.HPLC provides a promising tool for easy and reproducible permeability prediction that is amenable to high-throughput screening.

 

 

6/47 EVALUATION OF THE EFFECT OF EZETIMIBE, ATORVASTATIN AND THEIR COMBINATION ON EXPERIMENTALLY INDUCED MYOCARDIAL INFARCTIONIN RATS

El Sayed A. Abd Al Latif, Omaima M. Abd Allah, Samia M. El Shiaty

and Enas E. Shaaban

Department of Pharmacology and Therapeutics, Benha Faculty of Medicine,

Benha University  

Myocardial infarction (MI) is an irreversible injury or subsequent necrosis of myocardial cells due to interruption of blood supply to a part of the heart.The present study was designed to evaluate the effect of ezetimibe, atorvastatin and their combination on model of induced acute myocardial infraction with hypercholesterolemic rats. Animals were classified into group 1: control group which was subclassified to normal control group (received standered chow) and vehicle group (received 0.5% carboxy methyl cellulose), group 2: hypercholestermic group with no medication (induced by 2% cholesterol for 6 weeks) group 3: hypercholestermic infracted group with no medication (diseased group) (induced by s.c injection ofisoprenaline at a dose of 150mg/kg diluted in 2ml of saline), group 4: Atorvastatin (8mg/kg) pretreatedhypercholesterolemic infarcted group, group 5: Ezetimibe (1mg/kg) pretreatedhypercholesterolemic infarcted group, Group 6: Ezetimibe (1mg/kg) and atorvastatin (4mg/kg) pr-treated hypercholesterolemic infarcted group, Group 7: Ezetimibe (1mg/kg) and Atorvastatin (8mg/kg) pretreatedhypercholesterolemic infarcted group.. All tested drugs showed improvement of lipid profile, compared to control normal group and diseased group. All treated groups showed significant results compared to ezetimibe 1mg/kg alone. Regarding MI, Atorvastatin 8mg/kg only showed improvement of parameters of MI, butezetimibe had no role. Conclusively: All drugs used in this study improve lipid profile, while only atorvastatin high dose improve parameters of MI.

 

7/47 THE PHYTOCHEMICAL AND HYPOGLYCEMIC EFFECTS OF LUPINUS ALBUS L. GROWING IN ALBAHA

Mahmoud Hamed Mohamed, Hafez Ragab Madkour*, Saied Salah Al Sakari** and Naser Abd Allah Awad

Dept. of Pharmacognosy, College of Clinical Pharmacy, Albaha University

*Dept. of Biochemistry, College of Clinical Pharmacy, Albaha University

**Dept of Biology, Faculty of Science, Albaha University

Lupinus albus L. is considered as an important source for lupin alkaloids. These alkaloids have many therapeutic uses especially as hypoglycemic effect for diabetic patients. The researchers tried in this project to proceed phytochemical and hypoglycemic effects of Lupinus albusL. growing in Albaha (Saudi Arabia) and to compare with the published data for the Egyptian lupin. Thirteen alkaloids were isolated from the seeds of Lupinus albus L. cultivated in Albaha region viz. (+)-tetrahydrorhombifoline, (+)-17-oxolupanine, (+)-5,6-dehydrolupanine, (-) 11,12-seco-12,13-didehydromultiflorine, (+)-lupanine, (-)-multiflorine, (+) angustifoline, (-) albine, (+)- 13a-hydroxylupanine, (+)-ammondrine, (-)-13a-hydroxymultiflorine (-)-sparteine and (-)-13a-hydroxysparteine. Identification of these compounds was established by spectral techniques as well as comparison with authentic samples. The antihyperglycaemic effect of the total alcohol extract as well as the major alkaloids lupanine and multiflorine were tested. L. albus extract and lupanine showed slight and transient hypoglycemic effect while multiflorine proved to be an active and effective compound for decreasing the blood glucose level in diabetic mice and no effect observed in the normal mice.

 

 

 

8/47 HEPATOPROTECTIVE ACTIVITY OF THE HYDRO-METHANOL EXTRACT OF PLICOSEPALUS CURVIFLORUS

Jihan M. Badr, Hashem A. Hassanean and Abeer Hanafy*

Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Egypt.

*Department of Pharmacology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt

The aqueous - methanol extract (70%) of the whole parasitic plant Plicosepalus curviflorus (Loranthaceae) was evaluated for its hepatoprotective activity against liver damage induced by paracetamol in rats. The principle depends on the incidence of hepato-specific serum markers disturbance as a result of administration of paracetamol. This is attributed to the development of oxidative stress. The results of administration of the aqueous - methanol extract of Plicosepalus curviflorus were compared with the well known hepatoprotective agent silymarin. It was found that treatment of the rats with the aqueous - methanol extract of P. curviflorus prior to administration of paracetamol significantly reduced the disturbance in liver function which was assessed by measurement of total protein, total bilirubin, ALP, SGOT and SGPT. In addition, oxidative stress parameters were also evaluated. Animals were observed for any symptoms of toxicity to ensure safety of the plant extract.

 

 

 

9/47 COMPARATIVE STUDY BETWEENNEBIVOLOLAND LISINOPRIL IN EXPERIMENTALLY INDUCED DIABETIC NEPHROPATHY IN RATS

Mohamed E. Mansour, Mahmoud M. Elfouly, Omaima M. Abd Allah and

Abeer A. Eldeeb

Department of Pharmacology & Therapeutics, Benha Faculty of Medicine, Benha University.

The presentstudy was designed to compare the benificial prophylactic effects of nebivolol and lisinoprilon the progression of diabetic nephropathy in a rat model of streptozotocin induced diabetes. Animals were classified into normal control group (received no medication), Utreated diabetic group induced by administrated of single I.P injection 65mg/kg of streptozotocin "STZ", nebivolol treated diabetic group (2mg/kg p.o) for 7 weeks, lisinopril treated diabetic group (1mg/kg p.o) for 7 weeks. The obtained results in the current work revealed that induction of diabetes by streptozotocin resulted in significant increase of fasting blood glucose level (FBG), serum urea level, serum creatinine level, 24hrs urinary albumin excretion (UAE) and significantly decrease of renal blood flow (RBF).Significant changes in histological findings in the kidney as compared to control group. While nebivolol treated group showed decrease FBG, serum urea, serum creatinine levels, decrease UAE and significantly increased RBF. It shows improvement in the histological features as compared to untreated streptozotocin (STZ) induced diabetic nephropathy. Lisinopril treated group showed no changes in FBG level, significantly decreased serum urea level, serum creatinine level, decreased 24hrs UAE level and increased RBF. It also showed improvement in the histological features as compared to untreated streptozotocin (STZ) induced diabetic nephropathy. Nebivolol may replace ACE inhibitor as the first choice drug in diabetics for prevention of end organ damage.

 

 

 

10/47 ANTIMICROBIAL ACTIVITY OF SELECTED FUNGI ISOLATED FROM RED SEA MARINE ORGANISMS

Jihan M. Badr, Sameh S. Elhady*, Lamiaa A. Shaala**,Hani Z. Asfour***, Hashem A. Hassanean, Diaa T. A. Youssef*

Department of Pharmacognosy, Faculty of Pharmacy, University of Suez Canal, Ismailia 41522, Egypt

*Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia.

**Natural Products Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia

***Department of Medical Parasitology, Faculty of Medicine, Princess Al-Jawhara Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia

The present study focuses on antimicrobial activity of a number of fungi isolated from a number of sponges collected from Red Sea. The sponges were collected from Red Sea using SCUBA diving technique during July 2013. In order to ensure fungal isolates to be symbiotic when obtained, a surface sterilization of the marine tissues was performed. The sponge sample was washed with 5% sodium hypochlorite, followed by 70% ethanol, to ensure that epiphytic fungi were destroyed by the washing while the symbiotic fungi (if any) were not affected. The symbiotic fungi were isolated under completely aseptic conditions. SDA (Sabouraud Dextrose Agar) as a solid culture media were used for fungal growth. After that the fungal extracts were evaluated for the antimicrobial activity against Staphylococcus aureus and Candida albicans. A number of 69 fungi were evaluated by agar diffusion method. A number of the tested fungal extracts revealed activity against Staphylococcus aureus while others showed activity towards Candida albicans. Theactive fungi were identified to be subjected to further investigation.

 


11/47 COMPARATIVE STUDY BETWEENNEBIVOLOL AND LISINOPRIL IN EXPERIMENTALLY INDUCED MYOCARDIAL INFARCTIONIN RATS

Mohamed E. Mansour, Mahmoud M. Elfouly, Omaima M. Abd Allah and

Abeer A .Eldeeb

Department of Pharmacology & Therapeutics, Benha Faculty of Medicine, BenhaUniversity.

Myocardial infarction (MI) is an irreversible injury or subsequent necrosis of myocardial cells due to interruption of blood supply to a part of the heart.The present study was designed to compare the benificial therapeutic effects of nebivolol and lisinopril on the progression of myocardial infarction in a rat model of isoprenaline induced acute myocardial infarction. Animals were classified to normal control group (received normal saline), Myocardial infarcted group (s.c injection of isoprenaline at a dose of 150mg/kg/day diluted in 2ml of saline on two consecutive days with an interval of 24 hours between application, Nebivolol treated infarcted group(0.5mg/kg p.o.) once daily for 4 weeks after induction of MI, Lisinopril treated infarcted group (20mg/kg p.o.) once daily for 4 weeks after induction of MI. The obtained results in the current work revealed that induction of myocardial infarction by isoprenaline resulted in significant increase heart rate, decrease mean arterial blood pressure and increase T-wave voltageat the end of the study, while administration of nebivolol and lisinopril to myocardial infarcted rats for 4 weeks resulted in decrease of heart rate, decrease mean arterial blood pressure, decrease in T-wave voltageand improved histopathological changes of heart as compared to infarcted untreated rats. Administration of Nebivolol and lisinopril immediately afteroccurance of acute myocardial infarction prevent progression of the disease and improve pathological changes occurred in the heart.

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