Vol. 2, October, 1998.

alterner cialis et levitra 1/2 IMPROVEMENT OF GLUCOSE OXIDASE PRODUCTION

cialis m├ędicament au maroc BY Peniciltium notatum MUTANT UNDER THE EFFECT OF

osu generique viagra france SOME REGULARITY FACTORS

S.A. Khalaf

Botany Department, Faculty of Science, Zagazig University, Egypt

Nine Penidilium notatum -trains were recorded as glucose oxidase (GOD) producers among 12 P. nolaluin strains isolated from Egyptian fruits. The strain number 2 -which isolated from apple fruit, gave the maximum enzyme activity (1.81 U/ml) after 4 dayS on the basal medium (BSM). After mutagenization and selection, mutants of P. notalum S2 resistant to certain agents were obtained Six of the mutants showed increased GOD in comparison with the parental strain. The mutant DG-8 resistant to deoxy-D-glucose (1 mg/mi) gave the maximum activity of the enzyme. (2.94 U/mi). The over-production of GOD by P. notatum S2-DG-8 was obtained in medium containing sucrose (10%), peptone (1%) andCaCOs (4%).

peut on prendre du viagra a 30 ans 2/2 SERUM BILE ACIDS AND ESTRIOL IN avis sur levitra 10mg TOXAEMIAS OF PREGNANCY

*M.S. El-Harty, **Y.A.S. Wafa, ****T.Z. El-Baz and ***M.A. Afify

*Biochemistry, ** Obstetric and Gynecology, *** Tropical Medicine and **** General Medicine Departments, Faculty of Medicine, Al-Azhar University,

Cairo, Egypt.

Sixty pregnant women in their late trimester between 36 and 40 weeks gestation who attended the antenatal clinic at Bab-Al-Shaaria, and Al - Hussein University Hospitals were selected for this study. They were classified into group (A) control group (16 cases), Group (B) mild preeclampsia (20 cases), group (C) severe preeclampsia (14 cases) and group (D) (10 cases) had suffered from eclampsia. Patients with previous history of liver or kidney disease were excluded. All the above groups were assayed for fasting and post prandial bile acids (glycocholic acid, glycochenodeoxycholic acids) and fasting estriol levels by radioimmunoassay as well as row/ine liver and kidney functions. The level of glycocholic acid in group (A) in fasting and post prandial was 0.68 ±0.36, 1.33 ±0.92 umol/l (respectively) with a significant increase in post prandial stale P>0.02, while in group (B) it was 0.88 ± 0.35, 1.35 ± 0.58 umol/l with a significant increase in post prandial state P<0.01. Comparing the fasting and post prandial level between group A and B, there were no significant changes. (P>0.05), On the other hand the level of fasting glycocholic acids levels in group ( C ) was, 1.2 ± 0.88 and in group D 1.5 ±0.75 umol/l .with a significant increase in both groups than group A (P<O.Q2 and P<0.01 respectively The level of glycochenodeoxycholic acid in group (A) in fasting and post prandial was 0.59 ± 0.48, 1.43 ± 1.12 umol/l (respectively) with a significant increase in post prandial state P>0.02, while in group (B) it was 1.09 ± 1.01, 1.28 ±0.8 umol/l without significant changes. Comparing the fasting and post prandial level between group A and B, there were no significant changes. (P> 0.05), In groups C and D the fasting level was 0.89 ± 0.47 and 1.56 ± 1.44 umol/l with no significant changes between group A and C but in group D there was a significant increase with comparison to group A (P<0.05). The level of fasting serum free estriol in group (A), (B), (C) and group (D) were 40.7±25.7,28+17.9, 18.9 ±21.3and8.7±11.8 units respectively. It was found that there was no significant changes between groups A and B and there was a significant decrease in group C andD as compared to group A (P<0.05 and P<0.01 respectively.

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achat cialis en pharmacie 3/2 ENTEROTOXIGENICITY OF DIFFERENT viagra et risques STAPHYLOCOCCUS AUREUS viagra cialis ligne ECOVARS ISOLATED FROM levitra en pharmacie belge ALEXANDRIA SLAUGHTERHOUSES

LA. Samaha

Faculty of Veterinary Medicine, Alexandria University, Egypt.

Random samples (192 bovine, 96 ovine and 144 human) were collected to investigate the production of enterotoxins by different Staphylococcus aureus biotypes that isolated from carcass surfaces and workers. The results showed that the percentage of Staph. aureus isolated from slaughterhouse workers was found to be 27.8%, thirty percent of'those were enter otoxigenic. The percentages of these strains isolated from bovine and ovine samples were 8.9 and 6.3 in which 17.6 and 16.6% were enterotoxigenic. Staph. aureus strains were biotyped into bovine, ovine, porcine, human and non host specific ecovars at percentages of 30, 35, 5, 20 and 10% with the human samples, while these biotypes were 41.2, 17.6, 0.0, 17.6 and 23.5% within bovine samples and they were 33.3, 33.3, 16.6, 16.6and0.0% within ovine samples respectively. For these enterotoxigenic strains, it was found that 41.7% of the human biotype produced S.KA. (Staph. aureus enterotoxin A), 16.7%producedS.E.B and 25% produced S.KC. For the bovine ecovars 4.8% produced S.KA, S.E.B and S.E.D. On the other hand, 10.5% of the ovine ecovars produced S.E.C.S.E.D. and S.E.A+C were produced at percentagesof 25 and 37.5% from the non host specific ecovars, respectively. The public health significance of the isolated strains and their toxins were discussed.

4/2 EFFECT OF SUBLETHAL CONCENTRATIONS OF SOME PLANT

MOLLUSCICIDES ON SURVIVAL AND FECUNDITY OF BIOMPHALARIA ALEXANDRIA

H.A. El-Nahas

Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute,

Imbaba, Giza, Egypt.

Experiments -were conducted with LCo, LCioctndLC2i of Azudiruchtti indica, Glinus lotoides and Agave heteracantha plants to test their prolonged effect on both survival and fecundity of adult Biomphalaria alexandrina snails. The exposure period lasted 8 weeks followed by another 8 weeks as recovery. Results obtained showed that snails treated with the 3 plants suffered from great reduction in their survivorship during the exposure period. A. indica proved to have a more destructive effect than G. lotoides and A. heteracantha since snails exposed to only 40 ppm of the former plant died by the 6th week. The egg laying capacity and the total reproductive rate of the snails were markedly lowered than untreated snails, however the depression was more pronounced in snails exposed to relatively high concentrations. Also A. heteracantha was more effective than the other two plants in reducing snail fecundity as snails exposed to LCzs of this plant stopped egg lay ing for many weeks. It was also evident that this dual activity of the plants on survival and reproduction was irreversible during the recovery period. So the results were discussed in the light of the possible use of these low concentrations of the plants in interrupting of schistosomiasis transmission at relatively low cost.



5/2- EFFECT OF ORAL ADMINISTRATION OF FAGONIA

ARABICA EXTRACT ON CERTAIN BIOCHEMICAL VALUES

IN STREPTOZOTOCIN - INDUCED DIABETIC RATS

N.H. Mahmoud; H.S. Mostafa* and M.R. Elgindi**

Department of Physiology, Faculty of Medicine for Girls, Al-Azhar University; *Department of Biochemistry & Faculty of Medicine for Girls, Al-Azhar University and **Department of Pharmacognosy, Faculty of Pharmacy, Hellwan University

Since ancient times, diabetes mellitus (DM.) have been treated orally with plant extracts based on traditional folk medicine. This study is designed to assess the hypoglycemic effect of saponin extract jrom Fagonia Arabica plant on diabetic rats. The extract was given orally for 8 weeks to streptozotocin (STZ) diabetic adult male rats. The fasting blood glucose level, serum concentration of insulin, C-peptide, triglycerides, cholesterol, liver transaminases, urea and creatinine were estimated. In STZ diabetic rats, a significant increase in the fasting blood glucose, serum triglycerides, cholesterol, urea and creatinine observed associated with a significant decrease in serum insulin and C-peptide. Oral administration of the extract to the diabetic rats in a dose of 50 mg/kg. daily for 8 weeks induced a significant decrease in the fasting blood glucose, triglycerides, cholesterol, urea, creatinine and serum glutamate pyruvate transaminase (SGPT). An increase in serum insulin, C-peptide and serum glutamate oxalacetate transaminase (SCOT) were observed. However, blood glucose, serum triglycerides and SCOT level of the diabetic rats treated with the extract were significantly higher than that observed in the normal rats.

6/2 APPLICATION OF ENZYME INHIBITOR TECHNIQUE IN REGULATING tHE ACTIVITIES OF PURIFIED CONSTITUTIVE

PROTEASE PRODUCED BY STAPHYLOCOCCUS A UREUS 1-10

ISOLATED FROM A CLINICAL SOURCE

S. P.M. EI-Kastawy

Microbiology Department, Faculty of Pharmacy, AI-Azhar University, Nasr City,

Cairo, Egypt.

The aim ofihis study is to detect the effrct of enzyme inhibitors on the activity of purified constiluliveprotease responsible for bacterial pathogenic ity and to detect the parameters controlling such enzyme activity. The enzyme produced by the most potent Staph. aureui isolated from a clinical source was pu~rified using column chromatographic techniques. Parameters controlling the activity qfpiirified enzyme was tested The activity of enzyme decreased with the increases of enzyme dilution and substrate concentration. While activity was inhibited when incubation temperature was less than 20°C and more than 60°C. Also activity was inhibited by adding Cu"2, Co+2, Zn+2, Mn\ Ni+2, Hg+2, Co*2, Li+2 & AL+3 as enzyme inhibitors at all concentration used However, Mg+2, Fe+2andCa +2 were effective at concentrations > J 00-400 Og/ml. Whereas urea and EDTA at concentrations > 50-50 Og/ml. Finally, it was recommended that various studies for application of enzyme inhibitors due to its economical and efficient importance in medical andpharmace uticalfield must be subjected for further investigations.


7/2 ANTIMICROBIAL ACTIVITY OF GARLIC EXTRACT AGAINST CERTAIN PLANT-BORNE PATHOGENIC FUNGI

N.A. Younis; P.M. EI-Nashaby and A.M. El-Ashmawy

Plant Research Dept, Nuclear Research Center, Atomic Energy Authority,

Cairo, Egypt.

Seven plant borne fun gal pathogens viz. A. parasitiefis, A.flavus, A. niger, F. solan4 F. oxysporium, P. itaticum and P. oxalkum, they were isolatedfrom d~rent diseased fruits, tubers, plants and infected grains. Garlic extract used in controling its f~gal growth, it was lethal to all the testedpatho gens at 2.4% concentration. While 08% concentration was lethal~to all fungi except A. flavus, A. niger and P. oxalicum, the reduction ofmycelial dry weight of these fungi reached 81.8%, 7&4% and 86.5%. The same concentration completely inhibited protein content of most fungi under experiment except A. niger and P. oxalicunt. Percentage ofreduction of the ir protein content was 16% and 52.8% respectively Also this concentration completely inhibited cellulolytic and pectinolytic activity of A. parasitkus, F. oxysporium and P. italicum, while it decreased the activity of the other fungi. Garlic extract at concentration 3% can be efftctively used in keeping cucumber pickling quality durjng one month of 'storage, it had a very high protecting ability against the tested fungi and other microorganisms naturally infrcting pickling industry.

8/2 THYROID HORMONES AND PROLACTIN IN PRETERMS WITH RESPIRATORY DISTRESS SYNDROME

A.M. E. Moustafa; A. A. Gad and A. Abdel-Lateef

Health Research Department, National Center for Radiation Research and Technology

Cairo, Egypt

This study was conducted on thirty preterms suffering from respiratory distress syndrome (RDS) with gestational age between 32- 37 weeks (mean ±S.D. is 31.5 ± 2.21). Thirty healthy preterms served as a control group. Their gestational age ranged between 32- 37 weeks (mean ±S.D. is 34.7± 1.74). The objective of this study was to evaluate the thyroid hormone and prolactin levels in preterms with and without RDS. All preterms were subjected to complete history taking, history of maternal complications, mode of delivery and thorough physical examination with special emphasis on signs of respiratory insufficiency and Apgar score at 1 and 5 minutes. Serum levels of thyroid stimulating hormone (TSH), total triiodothyronine (T3), total thyroxin (T4), free thyroxin (FT4) and prolactin were measured during their first week of life using radio- immunoassay. The results of this study revealed that preterms with RDS had higher serum TSH levels compared with control infants (p< 0.001). Lower values of serum 1}, T4, FT4 and prolactin wasfound in preterms with RDS and this was significantly lower when compared with, control infants (p< 0.001). These data may indicate that preterms with RDS tend to be hypothyroid and hypoprolactin. This study also showed the presence of positive correlation between gestational age and T4, FT4 and prolactin. Thus we can suggest that administration of thyroid hormones and prolactin in preterms with RDS may stimulate the production of surfactant and lung maturation.


9/2 THE PREVALENCE OF FUNGAL INFECTION IN RESISTANT CORNEAL ULCER

A.H. Regaila, M.M. Yassin, T. Abdel-Hamid and A. Mounir*

Microbiology and Immunology Department and Ophthalmology* Department, Faculty of Medicine, Ain Shams University. Cairo, Egypt.

Fungi are responsible for a large proportion of suppurative cornea/ ulceration, which is a serious sight-threatening condition. The present work was done to estimate the prevalence of fungal infection in resistant cornea! ulcer and to evaluate the accuracy of some laboratory diagnostic techniques. A group of seventy patients complaining of resistant corneal ulcers was studied by taking cornea! swabs, corneal scrapings and corneal biopsy specimens from each one. All specimens were cultured on Sabouraud's Dextrose Agar "SDA" medium. In addition, corneal biopsy specimens were also directly stained with Ink-KOH and Calco-fluor White stains. Our findings showed that the prevalence of fungal infection in resistant corneal ulcers was 67.1%. Aspergillus species had the highest prevalence rate (68%), followed by Fusarium (21.3%), and finally Candida species (JO. 7%). Trauma, especially with a vegetable matter was the main predisposing factor for the development of fungal keratitis. We also found that corneal biopsy specimens of the seventy patients cultured on SDA. had the highest accuracy rate as they could diagnose 47 cases with fungal keratitis (67.1%). Calco-fluor White staining technique is also a sensitive method for diagnosing mycotic keratitis but it is relatively of high cost and needs special equipment.

10/2 THE ROLE OF INSULIN RECEPTORS IN THE EFFECT

OF GARLIC ON BLOOD GLUCOSE HOMEOSTASIS IN

NORMAL AND DIABETIC RATS

A.M. Ahmed * M.M. Farrag** and O.D. El-Gindi ***

* Radioisotope Department, Atomic Energy Agency, ** Physiology Department,

Faculty of Medicine, Al-Azhar University and *** Pharmacognosy Department,

Faculty of Pharmacy, Al-Azhar University.

When 100 mg/kg body weight/day of garlic oil was given to normal rats, it had hyperglycemic action, but when it was given to diabetic rats it produced hypoglycemic action. This study was designed to clarify the underlying mechanism of this confliction, by measuring the blood levels of glucose, insulin, glucagon and insulin receptors sensitivity in R.B.Cs and target tissues of insulin receptors (liver -muscle - adipose). Animals were divided into four groups: the first group was fed on ordinary rat diet and served as a control (A), the second one received garlic oil (100 mg/kg b. w/day) orally for 4 weeks, the third group was diabetic and served as control (B), the last one was diabetic and received garlic oil (100 mg/kg b.w day) orally for four weeks. The results showed that garlic oil in normal rats produced significant increase in glucose and insulin levels and significant decrease in glucagon level and insulin binding ability of the receptors at the RBCs, muscle, adipose and liver tissue 's receptors, while in diabetic rats produced significant decrease in glucose and glucagon levels and significant increase in insulin level and insulin binding ability. In conclusion, insulin receptors play an important role in the action of garlic oil on glucose homeostasis, as they have to decide at which side the garlic will act as hyper or hypoglycemic agent. The helping factor is the previous state of blood glucose level before ingest ion of garlic oil, i.e. if there is normal glucose level, an insulin resistance stale will arise as a protective mechanism, while if there is hyperglycemia (diabetes), garlic oil will help the body to reduce the blood glucose level with decrease the body's requirement to insulin.

11/2 ISOLATION AND PREVALENCE OF METRONIDAZOLE

RESISTANT HELICOBACTER PYLORI STRAINS AMONG

EGYPTIAN PATIENTS WITH GASTRITIS

AND PEPTIC ULCER DISEASE

E.A. Gouda*, A.A. Elzahaby**, A.A. Mohamed*** and M.M. Bazeed****

Microbiology *, Tropical Medicine**, General Surgery***, Internal Medicine**** Departments, Faculty of Medicine, Al Azhar University

Helicobacter pylori was successfully isolated on Dent's media from gastric biopsy specimens taken by endoscopy, in 38 out of 50 patients (76%) with gastritis or peptic ulcer disease. The overall metronidazole resistance rate was (60.5%) as tested by disk diffusion method on Muller-Hintonagar media supplemented by 5% whole sheep blood and incubated in microaerophilic atmosphere for 48 hours. Females showed a higher, though not significant rate of metronidazole resistant (65%) than males (55.5%) (p=0.37). Furthermore, patients with metronidazole resistant strains showed lower median age (37 years), compared to those with susceptible ones (48 years). However, the difference was not significant (p=0.06). Moreover, metronidazole resistant was not significantly affected by the types ofendoscopic diagnosis: doudenal ulcer (73.6%), gastric ulcer (100%), both doudenal and gastric ulcers (60%), and gastritis (38.4%), (p=0.20). Since metronidazole resistant Helicobacter pylori strains are frequent (60.5%), it is recommended to test metronidazole susceptibility before treatment to guide the selection of appropriate therapy and to avoid eradication failure of Helicobacter pylori infections.

12/2 EFFECT OF PYRITHIAMIN TREATMENT ON THE ACTIVITY OF TRANSKETOLASE IN RAT BRAIN

K.M. El-Nageh

Department of biochemistry, Faculty of Medicine, The Great Al Fateh University of

Medical Sciences, Tripoli, Libya.

Transketolase activity was determined in the brain of acutely thiamin-deficient rats, the mean activity was 61.7% of that in control rats. A connection between the decrease 'in transketolase activity in the brain of thiamin-deficient rats and the appearance of neurological disorder was suggested.

13/2 STUDY OF SOME TRACE ELEMENTS IN CHILDREN WITH UREMIA

A. Abdel-Lateef; A. A. Gad and A.M.E. Moustafa

Health Research Department, National Center for Radiation Research and Technology

This study was conducted on 35 children with chronic renal failure (CRF). Patients had a mean age of 6.96 ±2.7years (range 1.5- 12 years), 24 were boys while 11 were girls. They were subdivided into two subgroups: The first subgroup included 20 patients under conservative treatment for CRF, the second subgroup included 15 patients suffering from end stage renal disease and were under regular dialysis. Fifteen healthy children, age and sex matched with the patients served as controls. Patients as well as control were subjected to careful history taking, thorough clinical examination and laboratory investigations that covered ghmerular filtration rate, blood urea nitrogen, serum creatinine, total protein, albumin and globulin. Also, serum zinc (Zn), copper (Cu), magnesium (Mg) and manganese (Mn) were measured by Atomic absorption spectroscopy. The results showed that children with CRF had a significant decrease in Zn level and significant increase in serum levels ofCu andMg as compared to control. As regard to Mn, the difference between both groups was insignificant. This study highlighted on the correlations between trace elements levels and the degree of renal impairment, total protein and duration of disease in uremic children.

14/2 SERUM TRANSFERRIN IN PRETERM INFANTS WITH RESPIRATORY DISTRESS SYNDROME

A. Abdel-Lateef and E.M. Nassar

National Center for Radiation Research and Technology, Health Research Department

Respiratory distress syndrome (RDS) retains its leading position as the cause of mortality and morbidity in the newborns. Sera from 20 infants with RDS and twenty normal controls were examined by ELISA technique to determine the level of transferrin(TRF) in order to assess its role in prediction and management of the disease. In comparison with the control, serum TRF levels were significantly lower in RDS infants than control. Also there were significant positive correlation between serum TRF and gestational age, birth weight, serum iron (Fe) and haemoglobin (Hb) in both RDS and control groups. This study suggested that the amount of free iron in plasma due to TRF deficiency in preterms with RDS might be the determining factor in surfactant per-oxidation and this indicates providing enough TRF to infants with RDS as well as to infants predisposed to it.

15/2 INFLAMMATORY CYTOKINES AND NITRIC OXIDE IN CHILDREN WITH SEPSIS AND SEPTIC SHOCK

*M. Kantoush, * M. Abu-F'arrag, * M. Rowisha and ** G.A. El-Azab

* Paediatric Department, Tanta Faculty of Medicine ** Clinical Pharmacy Department. Tanta Faculty of Pharmacy. Tanta University. Egypt.

The objective of the present study was to investigate plasma pro-inflammatory cytokine Interleukin-6 [IL-6]; anti-inflammatory cytokine Interleukin-JO [IL-10] and nitric oxide [NO] in children with sepsis and septic shock Twenty-five patients with sepsis and 20 matched controls were enrolled Patients were subdivided into three groups; uncomplicated sepsis [n, 5]; sepsis syndrome [n, 10] and septic shock[n, JOJ. The following daily measures were done during the three study days period: i) Determination of plasma levels oflL-6; IL-JO and NO metabolites ( nitrite plus nitrate [N02/N03J); ii) Evaluation of cardiovascular functions through recording the degree of hypotension and by echocardiographic determination of cardiac index[CI] iii) Determination of Organ Dysfunction Index [ODI] score and iv) Follow-up for hospital mortality. Resulls of the study showed that [II In uncomplicated sepsis: plasma IL-6 showed significant elevated levels that correlated positively with a parallel significant rise of IL-10 levels; whereas plasma NOVN03 levels were comparable with controls. ODI score was nil and no mortality reported [II] Patients with sepsis -ndrome and early septic shock showed: significantly increased levels of plasma IL-6 that was accompanied by significantly reduced levels of IL-JO and significantly increased N02/N03 levels. [Ill] Patients with late septic shock showed: significantly reduced IL-6 levels that were accompanied by significantly increased plasma IL-10 and NO2/NO3 levels. This was associated with significant drop of CI; significant rise of ODI score and 60% mortality. Plasma NO^JNOS showed the only significant correlation with clinical and echocardiographic indices of cardiovascular dysfunction. Plasma IL-6, IL-JO and N02/N03 correlated significantly with ODI score as well as with non-survival. In conclusion: Proinflammatory cytokines - nitric oxide activation probably plays a pivotal role in the pathogenesis ofpaediatric sepsis and may strengthen the argument for cytokines - NO modulation as a treatment for critically-ill children with sepsis and septic shock.

16/2 SELECTION OF DOWN REGULATION PROTOCOL IN

OVARIAN STIMALATION FOR INTRA-CYTOPLASMIC

SPERM INJECTION (ICSI) PROGRAM

Y.A.S. Wafa and M.S. El-Harty

Faculty of Medicine, AL-Azhar University

There are many down regulation protocols used for ovarian stimulation in ICSI cycles The most commonly used protocols are either long (Luteal) or short (Follicular) down regulation protocol. The selection of suitable down regulation protocol is still a point of controversy. In this study 312 candidates were randomly allocated in one of the two protocols. Candidates of each group were divided into 2 groups according to the base line F.S.H. (below 6 m lU/ml or above 6 m lU/ml) It was found that long protocol is associated with better follicular development, more oocytes retrieved, and better pregnancy rate if base line F.S.H. is less than 6m lU/ml. short protocol gives better results when base line F.S.H. is more than 6m Rl/ml.

17/2 PURIFICATION AND PROPERTIES OF ST-PECTINASE

PRODUCED BY ASPERGILLUS NIGER, S-48 TAT FROM THE

PEELS OF SOLANUM TUBEROSUM (ST) UNDER SOLID

STATE FERMENTATION CONDITIONS

M.S. Ammar, S.S. Louboudy, M.S. Azab and M.M. Afifl

Bot. and Microbiol. Dept, Fac. of Science, AI-Azhar Univ., Cairo Egypt.

An attempt for obtaining purified pectinase(s) biosynthesized under solid state fermentation (5SF) from the waste peels of solatium tuber osum (ST) collected from the

kitchen wastes ofresturants was carried. Purification ofST-pectinase resulted in having a specific activity of2JJ3 units/mg protein/ml, corresponding to purification folds of & 24 times. Data recorded for the properties of the purified enzyme (s) in the reaction mixture are: incubation time J8h. at room tempei'ature; optimumpH, 4,. optimum pectin concentration, a J%, thermal stability, 60 ~, a continuous increase in enzyme activity due to the increae ofenzyme concetnration, ST-pectinase was stable at pH value of 5 using citrate-phosphate buffer. A comparative study inbehveen ST-Pectinase(s) and Novo-pectinex (one of the most common pectinases applied in food industries) has been undertaken using both P. C. Z and viscosometric techniques. A suggestion was given for the possible application of the present pectinase(s) obtained from Aspergillus niger, S-48 TAT in the fruit juices industry for the purpose of both yield and quality improvement of the resulting juices obtained from different fruits.


18/2 BIOSYNTHESIS, PURIFICATION AND CHARACTERIZATION OF EXTRACELLULAR CELLULASE BY RHIZOPUS NIGRICANS

S.S. Louboudy

Botany & Microbiology Department, Faculty of Science, Al-Azhar University,

Cairo, Egypt.

An investigation concerning fungal cellulase(s) biosynthesis, purification and characterization was carried ouL For such a purpose a screening pro gram for the ceilulytic activities of nine fungal strains viz: Penicillium ehiysoginum; Rhizopus nigricans; Fusarium moniliform, F. solani,; Agarkus sp.; Mucor sp.; Aspergillus Niger; A. flavus and A. parusiticus revealed that R nigricans is the most potent one. Optilal conditions controlling cellulase biosynthesis by R. nigricans are: incubation ten~perature 28°C; pH, 6; inoculum size 8.4 x 108; incubation time, 144h; Avicel concentration, 5 g/L. Purification steps included, crude enzyme preparation; fractional precipitation by ammonium sulfate; dialysis, and application of column chromatographic technique using sephadex G-200. This resulted in having a specific activity of 36.60 u/ml corresponding to 7. 42 purification folds. Data ofparameters qtfrcling the purified cellulase activity proved that the best Avicel concentration, 0.25%; optimum incubation period, J5h. and the enzyme was stable within 10-55°C with a maximum at 35°C.

19/2 CHARACTERIZATION OF STREPTOALLOTE1CHUS SR,

A HALOTOLERANT ACTINOMYCETE PRODUCING

oc- AMYLASE INHIBITOR

Z.K. Abd EL-Aziz

Botany and Microbiology Department, Faculty of Science for Girls, Al- Azhar University, Cairo, Egypt.

A moderately halophilic actinomycete strain was isolated from a salty soil sample collected from rhizospheric aerea of rare plant (Halopeplis peefoliata) obtained from Mersa Hemera, Halayb (Egypt) through a simple screening method which was adapted to detect oc- amylase inhibitor. The salinity and other properties of soil sample which was collected from mersa- hemera in Red-sea shore at shalatein -Halayb triangalar aerea were detected from mechanical, chemical and atomic absorption analysis. Taxonomical studies of the producing actinomycete strain with respect to the morphological, physiological, cultural characteristics and chemistry of cell wall analysis conform to the characteristics of genus. Streptoalloteichus, which until now has contained a single species, St. hintlustanus. Using scanning, transmission electron and photo microscopes indicated that the isolate is characterized by grayish yellowish pink to brownish pink aerial hyphae, yellowish creamy to grayish yellow and brown substrate mycelium with no soluble pigments on ISP media No. 2,4,5 and 6 respectively. This isolate is characterized by producing antimicrobial activities against Candida and some gram positive bacteria. The vegetative hyphae bear spherical sporangium like vessels wich enclose motile spores. The cell wall contains meso-diaminopimelic acid. Whole cell hydrolysates contain galactose, mannose and rhamnose (Cell wall chemotype III and type C whole cell sugar pattern). On the basis of the chemical, Physiological and morphological properties of this isolate, it was suggestive of being belonging to Streptoalloteichus hindustanus with some difference and it was giving the name Streptoalloteichus hindustanus sub. sp. halotolerans.


20/2 EFFICIENCY OF ULTRA VIOLET RADIATION AND

CHLORINE TREATMENTS FOR DISINFECTING OF

SWIMMING POOLS IN EGYPT.

A. A. Hassan and H.A. Hussien

National Center for Radiation Research and Technology, Cairo Egypt

Five public swimming pools in Cairo were examined in order to know the hygienic condition of their waters. The samples were taken daily for 7 days and examined microbiologically. The results revealed that the unsufficient of the chlorine concentration in pools water to eliminate the microorganisms. The free chlorine decreased from 0.6ppm in first day to about zero in the seventh day and the microbial load increased gradually till it reached more than 10^ cfu/mlfor bacteria and 10$ for fungi. Certain pathogens were detected namely; E. coli, Staph. aureus, Bacillus cereus, Candida albicans, Aspergillus flavus, and Aspergillus niger. Ultraviolet light has a significant effect on bacteria even the sporeformers and Candida albicans but results appeared the highly resistance of filamentous fungi to UV irradiation. It has recommended that for long time disinfection of swimming pools, the water should be chlorinated by at least 0.6ppm after recycling by UV light.

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