Vol. 5, April, 2000,

femme prend viagra 1/5 STUDY ON ANTICARDIOLIPIN ANTIBODIES IN PATIENTS WITH CEREBRAL STROKES

A.A. Youseff*, A.M. Agha, M.A. El Azzony, M.A. Afifi**

Departments of Clinical and Chemical Pahology* and Internal Medicine**

Benha Faculty of Medicine

           Cerebral strokes are group of serious diseases that affect the central nervous system. Antibodies directed against phospholipids are highly associated with many venous and arterial thrombotic episodes. Anticardiolipin antibodies (ACA) (IgG, IgMand IgA) are one of these. To study the incidence of AC A in patients with cerebral strokes for better etiological and prognostic criteria of this serious disease. 22 patients suffering from cerebro vasclas strokes, as evidenced by clinical picture and CT brain scanning, were subjected to full laboratory investigations including AC A levels, detected by ELISA technique. 22 Patients with stroke were included in this study. Fourteen were males and 8 females with mean ages 46.07 ± 13.22 and50.8+9.4 respectively. Seventeen patients were seen in their first attack and the remaining 5 in their second one. IgM and IgG levels of AC A were statistically significant in all patients in comparison to control group. Mean levls of IgM and IgG in patients in their first attack were 4.99 ±2.43, 14.21 ±11.98 in comparison to those in 2nd attack, 2.41 ± ,85, 7.2+3.39, respectively. Female patients showed higher levels of IgM and IgG antibodies in comparison to males being 5.213 ±1.192 and 17.145 ± 15.991, 3.946 ± 2.846 and 10.058 ±6.116 respectively. Measuring AC A level in patients with stroke is aval liable labor airoy test, which may carry certain diagnostic and prognotic importance including prevention of recurrence of such serious disease.

action du sildenafil 2/5 GASTRIC MUCOSAL SECRETION OF TUMOUR NECROSIS

acheter viagra pour femmes FACTOR-a, INTERLEUKIN-8 AND INTERLEUKIN-10 IN prix du viagra en baisse HELICOBACTER PYLORI qual é melhor levitra ou viagra ASSOCIATED CHRONIC GASTRITIS

S.M.S. El Nakeab», D.F.I. El Fouhil* and M.A.R. Abdel Kader*

•Biochemistry, *Microbiology and * General Medicine Departments, Faculty of Medicine for Girls, Al Azhar University, Nasr City, Cairo, Egypt.

The secretion of tumour necrosis factor-a (TNF-a), interleukin-8 (IL-8) and interleukin-10 (IL-10) by human gastric antral mucosa during short term in vitro culture of gastric antral mucosal biopsy specimens was investigated and measured by enzyme linked immunosorbent assay (ELISA) in thirty-seven patients with Helicobacter pylori cialis lilly prix (H. pylori) associated chronic gastritis compared to nineteen patients with wo bekomme ich cialis her H. pylori negative chronic gastritis and sixteen subjects with normal gastric mucosa and negative viagra donne forum H. pylori, H. pylori status was determined by microscopy, by biopsy urease test and by serology. TNF-a, IL-8 and IL-10 secretions were found to be significantly higher in patients with H. pylori associated chronic gastritis (p<0.0001) than in patients with cialis en ligne au canada H. pylori negative chronic gastritis and subjects with normal gastric mucosa. Raised TNF-a and IL-8 secretions may suggest their role in the inflammatory process associated with acheter viagra en turquie H. pylori infection, while raised IL-10 secretions may reflect its counter regulatory role leading to damping the body immune responses and persistence of H. pylori infection. Significant correlations were found between TNF-a and IL-8 secretions (r=0.9916), TNF-a and IL-10 secretions (r=0. 9842) as well as between IL-10 and IL-8 secretions (r=0. 9742) in patients with H. pylori associated chronic gastritis reflecting various relationships between these cytokines in those patients.

3/5 SOME ENVIRONMENTAL PARAMTERS INSIDE LABORATORY ANIMAL HOUSE

K . Abd El-Razek*; H. Samaha*; A . Draz* and A.M. Noweir**

* Dept., of Animal Hygiene and Zoonoses, Fac. Vet. Med. Alex. Univ. ** High Institute of Public Health, Alex. Univ.

To evaluate the hygienic conditions inside laboratory animal house. Two groups of laboratory animals (Rat and Mice) each group of 60 animals kept at the same room were used in such study .Some environmental parameter were measured. The obtained results revealed the following: Temperature was ranged from 19 - 32.2°C at level l (at the bottom of rack) with mean value of 24.7 ±1.41 C and ranged from 18,3-31,3C with means value of 24,1 ±1,23 at level 2 (at the top of rack), humidity at animal room was ranged from 48-77% with mean value of'64.4±1.21, ammonia level'was ranged from 1.6-15.4 ppm with mean value of 8.32+0.77 ppm, carbon monoxide level was ranged from 2.3 - 7.2 ppm with mean value of 4.36 ± 22 ppm, sulpher dioxide was ranged from 2 - 7.4 ppm with mean value of 2.98 ±0. J 7 ppm, carbon dioxide level was ranged from 1830-1960 ppm with a mean value of 189.88 ±5.76 ppm, bacterial count of air samples was ranged from 80-320 colony with mean 161.88 ± 9.63 and from 60-280 colony with mean 135 ±7.83 at level (1) (at the bottom of rack) and level (2) (at the top of rack) respectively, haemoglobin of tested rat and mice showed that 26.47 and 53.12% within normal Hb level respectively and Weighting tested rat and mice during 32 week showed it was differ significantly and highly significant/} rather than the standard weight of rat and mice.

4/5 EFFECT OF CADMIUM ON BUTRYLCHOLINESTERASE

ACTIVITY (KINETIC STUDIES)

A. A. Mahmod

Department of Biochemistry, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia

The inhibitory effect of cadmium on plasma BchE was investigated in the present study using the substrates butrythiocholine (BuTCh) andacetylthiocholine (AcTCh). Cadmium caused linear mixed type inhibition of human BchE with an IC50 of 1. J/u M. Inhibition kinetics of the enzyme by cadmium is studied in detail. The estimated inhibition constant (Ki) values were 0.56 uM for BTCh and2.6juMforATCh. The secondary plots of inhibition with respect to ATCh indicated a dissociation (KI) of 8.2 fjM for the enzyme-substrate complex. This KI being greater than the Ki suggests that the noncompetitive inhibition is predominant over the competitive inhibition at the substrate binding site.


5/5 RABBITS AS A RESERVOIR OF CERTAIN PATHOGENS OF ZOONOTIC IMPORTANCE TO MAN

*H.A. Samaha,*A.A. Draz,*Y.N. Hagagg,**B.G. Abdel-Aall

and **S.K. Ebied

*Dept. of Animal Hygiene and Zoonoses,Faculty of Veterinary Medicine,

AlexandriaUniversity.

**Animal health research institute,Alexandria branch.

A total of 173 pellet samples were collected from diarrhoeic (42) and non diarrhoeic rabbits (131) as well as 97 stool specimens were also collected from workers of rabbit farms and shop sailers and examined bacteriologically to identify' pathogenic and potentially pathogenic bacteria. In addition, 160 hair and skin scrapings from rabbits(124 clinically showing ring worm lesions and 36 apparently healthy rabbits) and 48 skin scrapings-from workers of rabbit farms clinically having tinea were examined mycologically and 136 ear sw'abs and skin scrapings collected from rabbits having clinical signs of mange and 54 skin scrapings from workers in rabbit farms having clinical signs of scabies were examined parasitologically. Bacteriological examination revealed that the isolation of Listeria spa. (2.31%), E.coli(20.81%) and Staph.aureus(l.l6%) from examined pellet samples of rabbits, - and isolation of Listeria spp.(6.19%), E.coli(18.56%), Salmonellaspp.(5.16%) and Shigella flexneri type6 (3.09%) from examined stool specimens of human. In addition, mycological examination revealed that the isolation of Trichophyton mentagrophytes(3.13%), T.schonleinii (1.88%), M.canis (1.25%), M. gypseum (1.88%) and T. simii (0.63%) from rabbits samples and isolation off. mentagrophytes . (10.42%), T.schonleinii (2.08%), T.simii (4.17%), T. rubrum(4.17%) and Candida albicans(4.17%) from examined human samples. Moreover, parasitological examination revealed that the demonstration ofSarcoptes scabieifrom body(40.44%) and Psoroptes cuniculi from ear(28.68%) of rabbits samples and Sarcoptes scabiei(44.44%) from human samples. The public health andzoonotic importance of these isolates were discussed.

6/5  STUDIES ON CERTAIN GRAM POSITIVE BACTERIA IN THE ATMOSPHERE OF KUWAIT DURING DUSTY PERIODS

M.Y. Al-Gounaim and A. Diab

College of Basic Education, P.O. Box 65002 Idailya, Kuwait

From the dust samples collected from the atmosphere of Kuwait during dusty periods, 408 bacterial strains were isolated, purified and identified. The mesophilic sporeforming Bacillus spp (118 isolate) were identified into seven species, of which Bacillus subtilis was the most frequent (42 strains of 35.6%) B. pumilus (21 strains of 17.8%), B. cereus (20 strains of 16.9%) and B. sphaericus (17 strains of 14.4%), other species were represented by 3-8%. The thermophilic Bacillus spp. (126 isolate) belonged to five species. B. coagulans (39 strains of 31 %), B. brevis (28 strains of 22 %), B. licheniformis (26 strains of 20.6%), B. stearothermophilus (25 strains of 19.8%), and B. badius (8 strains of 6.3%). The gram positive non-sporeforming rods (91 isolates) were identified into six genera, at which Arthrobacter was more common (44 isolates of 48.4), followed by Corynenbacterium (20 isolates of 22%). Other genera were represented 2-7 isolates. The gram positive cocci (68 isolates) were included in three genera: Micrococcus (39 isolates of 57.4%), Deinococcus (18 ioslates of 26.4%), and Staphylococcus (11 isolates of 16.2%). Micrococcus isolates included five species of which M. roseus was the most frequent (17 strains of 43.6%), followed by M. lutens (8 strains of 20.5%), M. kristinae (7 strains of 19%), Deinococcus isolates were identified as D. rodiodurans (10 strains of 55.5%), D. proteolyticus (5 strains of 12.8%) and D. radiophilus (3 strains of 7.7%) Staphylococcus included four species: S.aureus (6 strains of 15.4 %), S. epidermidis (3 strains of 7.7%), S. lentus and S. simulous (each of one strain).

7/5  SOME BACTERIAL HEALTH HAZARDS TRANSMISSIBLE

FROM RABBITS TO MAN

H.A. Samaha, A.A. Draz, E.G. Abdel All*, A.F. Farid** and S.K. Ebied*

* Animal Health Research Laboratory, Alexandria. ** Animal Health Research Institute, Dokki, Giza.

The role of rabbits in the transmission of some bacterial infections to hitman beings, in direct contact or dealing with those rabbits, was investigated. Bacteriological examination of 173 faecal pellets collected from diarrhoeic and apparently healthy rabbits, as well as 97 stool specimens collected from human beings working in rabbit farms and rabbit shop sailers in Alexandria area, was carried out. This was resulted in the isolation of Listeria species (2.31%), E. coli (20.81%) and Staph. aureus (1.16%) from faecal pellets of rabbits. On the other hand, Listeria sp. (6.19%), E. coli (18.56%), Salmonella sp. (5.16%) and Shigella flexneri type 6 (3.09%) were recovered from the examined stool specimens of human beings. The O-serogroups of E. coli strains isolated from rabbits were Ol, 02, 0128, 0119 and O127, meanwhile, O2, O26, Olll, 0119 and 0128 were recovered from the stool specimens of human beings. The hygienic measures that should be followed to prevent the transmission of such infections were also discussed.

8/5 DISTRIBUTION OF AIRBORNE BACTERIA AND ENDOTOXIN IN THE ATMOSPHERE OF THE STATE OF KUWAIT

M.Y. Al-Gounaim and A. Diab

College of Basic Education, P.O.Box 65002 Idailya, Kuwait

Airborne bacteria and endotoxin levels in the atmosphere of two districts (Fahaheel and Idailya) in the State of Kuwait vary considerably from locality.to another and month to month during the period of study. Maximum counts ofCFU/m3 of the mesophilic bacteria were 1429 ±50.2 and 1001 ±52.5from the atmosphere of Idailya and Fahaheel during January and November respectively. The thermophilic bacteria \verc at their higher concentrations during the period June - August in the atmosphere of the two localities. As a total, out of 725 and 719 bacterial cultures from Idailya and Fahaheel, 72.3% and 75.0% were starch hydrolysers, 23% and 14.5% were protein decomposers and 8.4% and 5.4% were Upases producers. The results show variations and no general trend could be obtained. The chromogenic bacteria in the atmosphere of Kuwait were in the ranges of 50.3% (in Idailya) -44.9% (in Fahaheel). The yellow and the orange groups were most frequent. The sporeforming bacteria isolated from the atmosphere of Kuwait were 419 isolates belonged to three genera: Amphibacillus (3.3%), Bacillus (93.6%) and Sporolactobacillus (2.6%). The genus Bacillus - the dominant one - was represented by 14 species (9 mesophilic and 5 thermophilic species), of which B. subtilis was more frequent (20.3%). This was followed by the thermophilic species, B. licheniformis (13.4%). The 89 isolates of the gram positive nonsporeformer rods were represented by 8 genera, of which Arthrobacter (39.3%) and Corynebacterium (21.3%) were more frequent. The gram-negative rods isolated from the atmosphere of Kuwait during the period of study were 60 isolates, of which 83.3% were isolated from the air of Idailya. Seven genera were identified. Flavobucterium was more frequent (36.7%), followed by Pseudomonas and Enterobacter (each of 23.3%). The 326 isolates of the gram-positive cocci included 6 genera. Micrococcus was highly represented (59.8%) followed by Staphylococcus (22.7%) and Deinococcus (J 7.4%). The genus Microccus included 8 species of which M. luteus was more frequent (29.7%) followed by M, roseus (18.5%). Under the genus Staphylococcus, 7 species were recorded. The more frequent species were S. saprophyticus (29.7%), S. epidermidis (25.0%) and S. aureus (17.6%). S. epidermidis, S. cupitis, S. hominis and S. warneri were recorded only from the atmosphere of Idailya. The endotoxin levels in the atmosphere of the residential district "Idailya" were estimated during the period of study. Maximum concentration of 520 ±20 EU/mJ air was recorded during April, while the lowest concentration was 165 EU/m3 during July. The relation between the endotoxin levels to different respiratory troubles was discussed.

9/5  THE CLINICAL UTILITY OF SERUM SOLUBLE

TRANSFERRIN RECEPTOR IN THE DIFFERENTIAL

DIAGNOSIS OF CANCER-RELATED ANEMIA.

N.M. Ghanayem*,   M.A.F. Hamodah*, M.A. AlAkaad**, W.M. Ibrahim"*

and E.A. Gawish**"

Departments of Biochemistry*&*", Faculties of Medicine, Menoufiya* and Tanta* Universities & Internal Medicine" .Zagazig University and Clinical Pathology*"*,Liver Institute, Menoufiya University.

Serum soluble transferrin receptor (sTf-R) is a quantitative marker of erythropoiesis and has been used to elucidate the pathophysiology of anemia in cancer patients. It may be useful as an adjunct in the evaluation of anemic patients whose ferritin values may be increased as the result of an acute phase reaction. This study was undertaken to determine the role of serum sTf-R in the diagnosis of cancer-related anemia. The mean transferrin receptor level in 15 normal controls was 28.27 ± 13.72 nmol/L compared with 64.2 ±10.77 nmol/L in patients with iron deficiency anemia ( p < 0.01). The mean serum transferrin receptor level was in the normal range in 40patients with cancer (20. J±8.87 nmol L). The mean sTf-R ferritm ratio levels were significantly higher in iron deficiency anemia patients compared to controls ( p < 0.001)) and cancer patients (p < 0.001) . STf-R/ferr i tin ratio by a cut off value of ' J had a sensitivity of 100% and a specificity of 100% in discriminating cancer related anemia from iron deficiency anemia. There wax an inverse significant correlation between sTf-R levels and all parameters of iron status in patients with iron deficiency while this inverse correlation is not found in cancer patients. It can be concluded that serum sTf-R is a reliable laboratory index of iron deficiency in cancer patients because serum ferritm levels are disproportionately elevated in relation to iron stores in patients with cancer.

10/5  ESTIMATION OF INTERLEUKIN - 6 IN CEREBROSPINAL FLUID OF MENINGITIS PATIENTS.

M.A.F. Moawad; A.A. Mohammed and O.A. Gaber*

National Center of Radiation Research and Technology. (N.C.R.R.T.) * Department of Medical Biochemistry, Faculty of Medicine, Zagazig University.

It is clear that cytokines exert a variety of modulator actions on the central nervous system. One of these cytokines is the interleukin-6. In this study, the role of inteiTeukin-6 (IL-6) in meningitis patients was investigated. 1L-6 levels in cerebrospinal fluid (CSF) samples from meningitis patients and control individuals were estimated. There was considerable elevation in IL-6 levels in all patients as compared to the control group. A significant difference was also found in IL-6 levels of CSF samples with bacterial growth when compared with samples of no growth.

11/5  RETROSPECTIVE STUDY OF INCIDENCE OF UPPER GASTROINTESTINAL BLEEDING IN AL-HUSSEIN

UNIVERSITY HOSPITAL, CAIRO, EGYPT.

(FROM SEP. 1995 TO SEP. 1998)

S. El Ebiary, M.B. Bastawi, A.A. Abdel Hameed and M.M. Hamza

Tropical Medicine Department, Al-Azhar University, Cairo, Egypt.

Upper gastrointestinal bleeding is often a dreadful experience not only for the patient but also for the relatives and not the least for the treating physician. It remains one of the most dijficult emergency situations faced by both physicians and surgeons. The purpose of this work was to study the magnitude of the bleeding and mortality in AI Hussein University Hospital all over the last 36 months in a retrospective study (from Sep. 1995 up to Sep. 1998). The study included 559 patients. For every patient a diagnostic upper endoscopy was performed to know the source of bleeding, hand in hand with the first aid treatment. Non variceal bleeding counted 35.5% of the aetiology of bleeding, most of them were males (71.6 %) in age group between 41 to 60 years old, most of them coming from urban area (72.7 %). Winter was the season of the highest rate (20 %). Non variceal causes were responsible for 6.9 % of cases. Upper GIT bleeding is a serious medical problem in Egypt, having mortality rate, which reached 20 % of all cases of our study. Although rupture of oesophago-gasthc varices was the most common cause of bleeding, the non-variceal bleeding constitutes a considerable percent especially in urban area.


12/5 ESTIMATION OF URINARY URODILATIN IN PATIENTS WITH HEPATORENAL SYNDROME

K. M. Abd El-Hafeyz, A.A. Abd Allah, M.A. El-Guindy and.A.K.T. Farrag

Biochemistry* and Tropical Medicine Departments Faculty of Medicine Tanta and Al-Azhar Universities

Urodilatin is a peptide formed in the kidneys, that shows marked diuretic and natriuretic functions. Recent studies indicate that urodilatin may be effective in patients suffering from hepato-renal syndrome. To emphasize the role of urodilatin, we conducted this work on 20 patients with cirrhosis and ascites. They were divided into 2 matching groups: Group 1 consisted of 10 patients with liver cirrhosis and ascites but without evidence of renal function impairment. Group II consisted of 10 patients with liver cirrhosis and ascites who showed evident hepatorenal syndrome. A third control group was included. Urinary urodilatin and sodium were measured. Analysis of urinary sodium was 12.45 ± 2.34 MEQ/L/day, 6.35 ± 1.7 6 MEQ/L/day and 35.9 ± 8.67 MEQ/L/day for groups I,II and control respectively. Urinary urodilatin, as measured by RIA, was 89.5 ±8.2 pmol/g creatinine, 55.7±9.65 pmol/g crealinine and 91.3 ± 6.43 pmol/g creatinine for groups I, II and control respectively.

13/5 ADRENOMEDULLIN: A NEW CULPRIT FOR EFFECTIVE

HYPOVOLEMIA IN ASCITIC PATIENTS WITH LIVER CIRRHOSIS

K.M. Abd El-Hafeyz, A.A. Abd Allah, A.M. El-Guindy, and A.K.T. Farrag

Biochemistry* and Tropical Medicine Departments Faculty of Medicine Tanta and Al-Azhar Universities

Adrenomedullin is a potent vasodilator pep tide that was recently discovered in human phechromocytoma. To study the role of adrenomedullin as a possible culprit for vasodilatation seen in cirrhotic patients, we conducted this work on 26 patients with post hepatilic cirrhosis, divided into 2 groups: Group I consisted of 15 patients with liver cirrhosis and no ascites. Group II consisted of 11 patients with liver cirrhosis and ascites. A third group of 7 healthy persons served as a control group. We measured Plasma renin activity and adrenomedullin in sera of these patients. Plasma renin activity was 8.86± 3.36, 16.4 + 7.24and 19.54 ± 8.37 tig/ml1 hf! in control, group I and group II respectively. Serum adrenomedullin was 6.3 ± 0.6, 7.5 ±2.3 and 12.7 ±4.3fmol/ml in control, group I and group II respectively. There was a significant positive correlation between adrenomedullin and PllA (r^O.824) and heart rate (r~0.654). There was a significant negative correlation between adrenomedullin and mean arterial blood pressure (r=0.76).

14/5  AEROBIC BACTERIAL SPECIES CAUSING BURN AND SURGICAL WOUND INFECTIONS AT THREE HOSPITALS IN UPPER EGYPT

W.K.A. Mohamed, T.R. EL-Khamissy, G.F. Mahmoud and M.S.E. Ashour

Department of Microbiology, Faculty of Pharmacy Al-Azhar University,

Cairo and Assiut

Five hundred sixty one burn and surgical wounds, from patients admitted at Assiut University Hospital (AUH), Sohag University Hospital (SUH) andAkhmim Central Hospital (AkCH). They were investigated for hospital-acquired wound infections. Out of three hundred and six cases (232 surgical and 74 burn wounds) from AUH developed 79 (25.8%) infections, comprising 52 out of 232 (22.4%) surgical wounds and 27 out of 74 (36.5%) burn wounds. In addition, 31 infections out of 83 (3 7.3 %) surgical wounds from SUH and 73 infections out of J 72 (42.4%) burn wounds from AkCH, were developed. Rate of surgical wound infections was higher in males (31.9%) than in females (16.9%), while burn wound infection was higher in females (42.9%) than in males (37.4%). Hospital-acquired infection were higher in old age (50%) and young age (41.3%) than in middle age (26.9%). Incidence of infections was increased with increase of extent of burn. Two hundreds and twenty eight strains were isolated from 183 burn and surgical wound infections comprising: 101 isolates of Pseudomonas aeruginosa, 53 Staphylococcus aureus, 34 E. coll, 25 Klebsiella pneumonia and 15 strains of Proteus mirabilis. Antimicrobial susceptibility of different bacterial isolates showed increased resistance to the commonly used fi-lactams (Ampicillin, Ampicillin/clavulanic acid, cefadroxil, cefuroxime, cefoperazone), chloramphenicol and trimethoprime/ sulfamethoxazole and less resistance to imipenem and ciprofloxacin. The degree of pathogenicity test of pseudomonas aeruginosa to mice showed no correlation with the antimicrobial susceptibility pattern.

15/5  ISCHEMIA-REPERFUSION INDUCED DYSFUNCTION IN RABBIT AORTA.THE PROTECTIVE EFFECT OF VERAPAMIL,

AND ASCORBIC ACID

H.H. Abu Rahama*, A.M. M. Metwally*, M.A. Mohammed* and R.A. Hemeida**

Departments of Pharmacology, Faculties of Medicine* and Pharmacy**,

Al-Azhar University (Assiut).

Many organs and tissues are likely to be exposed to intermittent ischemic episodes. During the resperfusion period (after ischemia), several mediators e.g. oxygenated free, radicals are known to be released and produce harmful effects more than ischemia per se. These mediators are thought to be released as a response to the excessive increase of the calcuim influx into the cells, hi the present study a pharmacological model of ischemia (deprivation of oxygen and glucose was applied to mimic the acute effects of reduced blood flow to investigate possible effects of ischemia-reperfusion on the reactivity of the isolated rabbit aortic rings to the contractile agent noradrenaline and to both the endothelium-dependent (acetylcholine) and -independent (sodium nitroprusside) vasorelaxant agents and to study possible protective effect of the calcuim channel blacker (verapamil) and the antioxidants (ascorbic acid) against these possible vascular dysfunctions caused by ischemia-reperfusion. Results of our study revealed that 30 minutes of ischemia followed by Ihr of reperfusion produced a highly significant impairment (P< 0.001) of the endothelium-dependent vasorelaxation induced by acetylcholine. This effect was reduced significantly (P 0.01) by pretreatment with verapamil (2 /jg). Ascorbic acid pretreatment (5 /jg) produced also a high significant (P 0.001) inhibitory action on these harmful effects of ischemia-reperfusion on the endothelium -dependent vasorelaxation. However, ischemia-reperfusion didn 'l affect significantly the endothelium-independent vasorelaxation induced by sodium nitroprusside. On the other hand, ischemia reperfusion produced signifiant (P < 0.05) potentiationfor the noradrenaline contractile effect on the isolated aortic ring preparations. This potential ion was significantly (P 0.05) inhibited by either verapamil or ascorbic acid. Results of the present study suggest that verapamil and ascorbic acid pretreatment may give a beneficial protective effects if used as a prophylactic therapy against harmful effects of ischemia - reperfusion in patients susceptible to ischemic episodes or to preserve the viability of tissue during organ transplant!on.

16/5  STUDY OF THE HEMATOLOGICAL INDICES AND SOME TRACE ELEMENTS IN CHILDREN WITH PROTEIN ENERGY

MALNUTRITION

A.M.E. Moustafa*; A.A.Gad** and L.M.Anis***

"(Childhood studies), **(Medical science), ***Health Research, Health Research Department, National Center for Radiation Research and Technology

Zinc, copper and iron are essential micronittrients for the development and growth as well as being necessary for the immune system and as an antioxidant defense. To evaluate the role of these trace elements 25 children suffering from protein energy malnutrition (PEM) were studied. Fifteen patients were mar asm ic (10 males and 5 females). Their ages ranged between 7 months and 36 months. Ten patients suffered from kwashiorkor (6 males and 4 females). Their ages ranged between 9 months and 24 months. Ten apparently healthy children sex and age matched with the patients were served as a control group. Both the cases and the control groups were subjected to careful diet history, anthropometric measurements, and clinical examination. Complete blood picture was done. The serum zinc (Zn), iron (Fe) and copper (Cu) levels were estimated using the atomic absorption spectroscopy. The results of this study showed that the levels ofHb, RBCS. count and haematocrite values were lowered in the malnourished infants. Also, the total leucocytic count was increased in the PEM cases. Children with malnutrition had significant lower values of Zn, Cu and Fe when compared to the control group. A significant positive correlation was found between serum Cu value and degree of malnutrition. Thus, it can be used as an indicator for the severity ofmalmitrition.

17/5  MICROBIOLOGICAL EVALUATION OF READY TO USE FROZEN MEALS IN ALEXANDRIA MARKETS

LA. Samaha and E.G. Abdel-All

Department of Food Control, Faculty of Veterinary Medicine, Alexandria University

The sanitary condition and the quality of ready to use or precooked frozen meat, chicken and fish meals sold at supermarkets and shops of Alexandria, were assessed through the microbiological evaluation of 93 (31 from each product) samples collected from these locations. The examination included the total aerobic bacterial count, total psychrotrophic count, Entero-bacteriaceae count, coliform count, Staphylococcus aureus count and the total mould and yeast counts. The obtained results revealed that the microbial counts of the examined products were within the recommended international limits. Moreover, the health importance of the microbial isolates and the hygienic measures that should be practiced during handling, processing, storage and marketing of these products, were also discussed.


18/5  BIOGENIC AMINES, AMMONIA AND NON PROTEIN NITROGENOUS COMPOUND CONTENTS OF MARKETED WHOLE CHICKEN, CHICKEN FILLET AND LIVER IN RELATION TO BACTERIAL COUNTS AND TEMPERATURE

E.A. Saleh* and Shahata A. Ahmed **

*Department of Food Hygiene, Fac. Vet. Med., Alex. University

** Food Control Department, Fac. Vet. Med., Cairo University

One hundred eighty random samples of whole chicken, chicken fillet and liver (60 each) were collected from retail shops (marketing refrigerator) and street vendors (room temperature) at Alexandria and El-Behera governorates. The bacterial counts, biogenic amines, ammonia andNPN %for these samples were evaluated. The results showed that, the livers have the highest bacterial counts which decrease successively in chicken fillet and whole chickens. The different bacterial counts for samples collected^ from retail shops were much lower than those of street vendors. The formation of biogenic amines was directly correlated to different bacterial counts and marketing temperature with the exception of both spermine and spermidine which showed no characteristic changes. Tryptamine could not be detected at all. In street vendor samples, cadaverine was the most predominant biogenic amine followed by putrescine, tyrarnine and much lower histamine and fl-phenylethylamine but in retail chicken samples only putrescine was slightly higher than cadaverine. Biogenic amines, ammonia contents and non-protein nitrogenous compounds (NPN) percentage were much higher in street vended samples than those of retailed samples. The public health significance of biogenic amines, ammonia and NPN compounds in relation to bacterial count and marketing temperature were discussed.

19/5  PLASMA LEVEL OF B-ENDORPHIN IN PREECLAMPTIC AND ECLAMPTIC TOXAEMIAS

I.T. El Garhy and W. Hablas

Obstetrics and Gynaecology Department and Clinical Pathology Department

Al-Azhar University

B-endorphin (B-EP) appears to play an important role in central nervous system as well as cardiovascular regulatory functions. B-EP levels were reported to increase progressively in normal gestation with marked increase in cases of pregnancy induced hypertension. The present work studies the changes in plasma B-EP in preeclamptic and eclamptic patients. Sixty pregtiant females among those attending Obstetrics and Gynaecology Department of El Husien Hospital Al-Azhar University participated in this work. They were classified into 3 groups. Group I included 25 normal pregnant females. Group II included 25 pregnant women with preeclemptic toxaemia. Group III comprised 10 pregnant women presented by or had a history of recent eclamptic fits or coma. B-EP was estimated using I12i plasma B-EP radioimmunoassay. Plasma B-EP showed a significant increase (P<0.01) in preeclamptic cases (12.6+8.5 pmol/L) and (P<0.001) in eclamptic cases (20.7±16.5 pmol/L) over that level reported in normal pregnancy (6.8+3.1 pmol/L). Plasma B-EP levels in eclamptic patients were higher than in preeclamptic ones but the increase was not statistically significant (P>0.05). these data indicate that B-EP system is markedly activated in these stressful conditions as a physiological mechanisms that might guard against more rise in blood pressure and occurrence of fits.

20/5  SERUM LEVELS OF MATRIX METALLOPROTEINASE 9

(MMP-9) AND TISSUE INHIBITOR METALLOPROTEINASE 1

(TIMP-1) IN LUNG CANCER PATIENTS: CORRELATION WITH

CARCINOEMBRYONIC ANTIGEN (CEA) LEVEL

M.A. Mohamed*, N.A. Fahim*, L.A. Helalah**, T.A. El Nagar**, M.M. Ahmed**

andM.F. El-Sayed***

Departments of Microbiology & Immunology *, Chest **, and Internal Medicine *** Faculty of Medicine, Ain Shams University, Cairo, Egypt.

There is evidence of the dysregulation of the balanced interaction between matrix metalloproteinases     (MMPs)     and   their   inhibitors,     tissue     inhibitors   of metalloproteinases (TIMPs) in cancer which is thought to facilitate tumor cell invasion and metastasis. The role ofMMP-9 and TIMP-1 was evaluated in different pathological types of lung cancer. Sera were collected from 52 lung cancer patients (10 had large cell carcinoma, 9 small cell carcinoma, 19 squamous cell carcinoma and 14 metastatic adenocarcinoma) as well as from 20 healthy control subjects. MMP-9 and TIMP-1 serum levels were measured using a sandwich enzyme immunoassay technique. Serum level of carcinoembryonic antigen (CEA) was also measured in the same subjects using a one step sandwich enzyme immunoassay technique. The study revealed a significant increase in the serum level ofMMP9 in lung cancer patients as compared to controls (P<0.001), with the highest value being found in patients with large cell carcinoma. A significant increase in TIMP-1 level was also found in lung cancer patients (P<0.001), with no marked variation amongst different pathological types. A significant increase was also found in MMP9/TIMP1 ratio in patients versus controls (P<0.001) with the highest value being measured in patients with large cell carcinoma. A significant positive correlation was found between the serum level of MMP-9 and TIMP-1 (r = 0.3, P< 0.05) and also with MMP-9/TIMP-1 ratio (r = 0.99, P<0.05) in lung cancer patients. Regarding CEA level, it showed a significant increase in lung cancer patients as compared to controls (P<0.05), with the highest value being obtained from patients with squamous cell carcinoma. No significant correlation was found between CEA serum level and either MMP9 or TIMP1 (P>0.05). Analysis of the diagnostic value of CEA, MMP-9 and TIMP-1 in lung cancer patients revealed that CEA had 40.38% sensitivity, 100% specificity and positive predictive value (PPV), 39.2% negative predictive value (NPV) and 56.4% diagnostic efficiency (DE). On the other hand, MMP-9 had 90.4% sensitivity, 95% specificity, 97.9% PPV, 82.6% NPVand 91.1% DE. The sensitivity, specificity, PPV, NPV and DE of TIMP-1 were 98.1%, 95%, 98.1%, 95% and 97.2% respectively. It can be concluded that increased serum levels of MMP-9, TIMP-1 and their ratio are found in lung cancer patients independently of CEA level. Both MMP-9 and TIMP-1 may serve as tumor markers, with TIMP-1 seems to be the most efficient with the highest diagnostic efficiency.

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