Vol. 9, April, 2002.

sur quel site acheter kamagra 1/9 ANTIADHERENT ACTIVITY OF STREPTOMYCES ERYTHROGRISEUS AGAINST GLYCOGALYX PRODUCER PSEUDOMONAS AERUGINOSA

M.M. Hafez, N.A. Hassouna and M.A.M. Yassien

Department of Microbiology and Immunology, Faculty of Pharmacy,

Ain Shams University

Primary screening of the antiadherent activity of 263 streptomycetal isolate was carried out using spectrophotometric method. The streptomycetal isolate that had the strongest antiadherent activity was selected for further studies. The selected streptomycetal isolate, identified as sildenafil accord 100 mg pris Streptomyces erythrogriseus, reduced the optical density of adhering and preadhered biofilms of meilleur pub pour le viagra Pseudomonas aeruginosa to 28.7% and 29.1% of that of control respectively. The same strong antiadherent activity of S. erythrogriseus isolate was observed upon using static adhesion assay, as it reduced the number of adherent cells and preadherent cells to urinary catheters to 27.4 % and 41% of that of control, respectively. The results of studying the effect of different factors showed that the optimum condition of antiadherent productivity of S. erythrogriseus was obtained by using soybean meal (M1) medium supplemented with 0.8 mM ZnSO4 and adjusted to pH 6.8 using 0.2M phosphate buffer and incubated at 28OC for 72 hrs. levitra et probleme cardiaque S. erythrogriseus has no antibacterial activity against propecia et alostil 5 E. coli ATCC 25922, achat sildenafil en france P. aeruginosa ATCC 27853, the selected glycocalyx producer cialis en espagne forum P. aeruginosa P6 , and ma femme à pris du viagra Staphylococcus aureus (ATCC 2821).

 

 

cialis remboursement ss 2/9 MOLLUSCICIDAL ACTIVITIES OF SOME EGYPTIAN PLANTS

H.A. El-Nahas, M.M. Abdel-Gawad, M.M. El-Sayed, and E.S. Abdel-Hameed,

Department of Medicinal Chemistry, Theodor Bilharz Research Institute

Warrak El-Hadar, Imbaba, Giza, Egypt.

Preliminary molluscicidal screening of the aqueous suspensions of the dry powder of some Egyptian plants against explication de viagra Biomphalaria alexandrina snails, the intermediate host of Schistosoma mansoni in Egypt revealed that Furcraea gigantea (Family Agavaceae) and Oreopanax reticulatum (Family Araliaceae) have considerable activities (LC90 = 130 and 160 ppm) after 24 hours exposure. Biological tests of different extracts such as methanol, acetone, ethyl acetate ,ether, chloroform , benzene and petroleum ether of Furcraea gigantea and Oreopanax reticulatum against Biomphalaria alexandrina snails appeared that only the methanol extract of the   F. gigantea has high activity (LC90 = 60 ppm) whereas other plant extracts are molluscicidally inactive up to 200 ppm after   24 hours exposure. All extracts of O. reticulatum did not show any activity up to 200 ppm after 24 hours exposure. Also the crude saponin of each plant was prepared and examined against B. alexandrina snails. Studying the effect of some environmental factors on the potency of the aqueous suspensions of each plant; F. gigantea and O. reticulatum showed that the activities of the two plants are almost stable. Owing to the high potency of the two plants against B. alexandrina snails, separation and identification of their active constituents is now being carried out.


3/9 PHYSICAL DEVELOPMENT AND ERUPTION OF DECIDUOUS DENTITION

M. Rashed* and S. Darwish**

National Nutrition Institute* and Ahmad Maher Teaching Hospital**

Malnutrition is a common nutritional problem in developing countries. The link between food choice and masticatory efficiency has long been established, however, the relationship between delayed eruption of deciduous teeth and choice of weaning foods has not been investigated. This work was designed to study the relationship between malnutrition and delayed eruption of deciduous teeth and the effect of delayed tooth eruption on mother’s choices of weaning foods. The study included a group of infants with delayed eruption of deciduous teeth and a control group with normal teething. Anthropometric measurements were taken in the form of weight and length. The form of weaning foods was recorded. Socioeconomic data was also collected. The results showed that infants with delayed eruption of deciduous teeth had significantly more stunting and underweight than infants of control group. The results also showed that there was a significant difference in the form of food given to the child between the delayed eruption group and the control group. The difference was statistically significant till the age of 12 months. It was concluded that infants with malnutrition had delayed eruption of deciduous teeth compared to normal infants. Delayed eruption of teeth in turn adds to malnutrition through difficult mastication and introduction of more diluted foods. We recommend that more attention be paid to delayed eruption of deciduous teeth, not only as a sign of delayed development but also as a contributing factor to infant malnutrition. Further study of the relationship between teeth eruption and energy density of food as well as the relationship between malnutrition, delayed eruption of teeth on one hand, and later development of dental caries on the other hand is also recommended.

4/9 PREDICTION OF SUCCESSFUL LABOR INDUCTION THROUGH ULTRASONOGRAPHIC MEASUREMENT OF CERVICAL LENGTH

K.M. Bayoumy, S.A. Mahdy*

Department of Obstetrics and Gynecology, *Department of Radiology,

Ain Shams University

The current study was done in Ain Shams University Hospital and included 30 patients admitted with post-date for induction of labor. Patients with rupture of membranes malpresentations or fetal distress were excluded from the start. In all patients cervical length was detected by transvaginal U/S before starting labor induction, which was done using vaginal prostaglandin tablets. The results obtained showed that 20 out of the 30 studied patients had cervical length <3 cm. That group needed lower doses of prostaglandins for labor induction. Their latent stage (from insertion of vaginal prostaglandins to onset of labor) and their induction to delivery duration were much shorter than patients who had cervical length >3 cm. The mode of delivery in all those patients was by vaginal delivery. The present study showed highly significant correlations between cervical length and both the Bishop score and duration of labor, it also showed that cervical length had a better predictive value and higher sensitivity and specificity results, than Bishop score in terms of predicting successful induction of labor.

5/9 INTERLEUKIN-6 AND TUMOR NECROSIS FACTOR IN WOMEN WITH PRE-ECLAMPSIA

A.M.N. Hashad, K.M. Bayoumy, H.M. Sammour and I.Y Abd El-Messih*.

Department of Obstetrics & Gynecology, Ain Shams University, * Department of Clinical Pathology, Ain Shams University

This study included 60 pregnant women divided into 2 groups: a control group (group I) which included 20 pregnant women with no medical or obstetric complications and patient group (group II) which included 40pregnant patients suffering from preeclampsia and had no other medical or obstetric complications; this group was further subdivided into 20 patients with mild preeclampsia and 20 patients with severe preeclampsia. All groups were assessed by complete history and full general and abdominal examination, urine sample was taken for presence of proteinuria and a venous blood sample was withdrawn for measurement of complete blood picture, blood sugar, liver function tests, renal function tests, PT, PTT, FDPs, LDH, TNF-a and IL-6. The results were statistically analyzed and the following relations were obtained: There was no significant difference between patient and control groups regarding Hb%, ALT, AST, blood urea and TNF level (p<0.05) and there was significant difference between them regarding serum creatinine, FDPs and IL-6 (p<0.05). From these results, measurement of TNF-a itself in the serum is not suitable for prediction of preeclampsia. Further studies on circulating TNF receptors are indicated as these persist more in the circulation than free TNF-a itself. Measurement of serum interleukin-6 (IL-6) in patients susceptible to preeclampsia is proved to be beneficial.

6/9 EVALUATION OF CEREBROSPINAL FLUID (CSF) INTERLUKIN-6 (IL-6) AND NITRITE IN DIAGNOSIS OF ACUTE PEDIATRIC MENINGITIS

A. Abdel- Lateef*; A.M.E. Moustafa*; M.M..Dawood** and L.M. Anis***

*(Childhood studies). ***Health Research. From Health Research Department, National Center for Radiation Research and Technology

**(Medical Biochemistry Department, Faculty of medicine, Zagazig University

The outcome of acute meningitis depends mainly on its early diagnosis and prompt treatment. We aimed to test the reliability of CSF, interlukin-6 (IL-6) and nitrite levels in early diagnosis of acute meningitis and its differentiation between bacterial (BM) and viral (VM) types. For this purpose, 40 patients were subjected to thorough physical and neurological examination. Lumbar puncture was done and CSF was examined bacteriologically, cytologically, biochemically, in addition to (IL-6) and nitrite estimation. According to the results of CSF analysis, the patients were classified into 3 groups: acute bacterial meningitis [20patients], acute viral meningitis [10 patients] and the control group [10 patients], they were suspected clinically to have meningitis but proved to be free by CSF examination. Clinical manifestations were similar in all groups. Biochemical and cytological results showed significant difference between the 3 groups. CSF IL-6 level was significantly elevated in meningitis groups (BM, VM) than the control group. Also, there was a high significant difference in CSF IL-6 level between BM and VM. The highest level was among BM group (mean ± SD; 4233.64 ± 2380.68 pg/ml) followed by VM group (mean ± SD; 184.99± 66.12 pg/ml) and lastly the control group (mean ± SD; 37.86± 23.6 pg/ml). As regards CSF nitrite levels, BM group had the highest level (mean ± SD; 29.54± 15.46 μ mol/L), then the control group (mean ± SD; 4.13± 2.55 μ mol/L) and lastly VM group (mean ± SD; 2.67± 1.21 μ mol/L) with a significant difference between the all three studied groups. We concluded that CSF IL-6 is a good marker for rapid and early diagnosis of acute meningitis. Concerning its role in differentiation between BM and VM groups, it has not yet been estimated. C.S.F. nitrite is not only a diagnostic marker of acute meningitis, but also it differentiates between its previous two types.

7/9 PRODUCTION OF HIGH SELENIUM YEAST IN PILOT SCALE BATCH FERMENTATION

A.I. El-Batal and M. Fadel*

National Center for Radiation Research and Technology (NCRRT). P. O. Box 29, Nasr City, Cairo, Egypt.

* National Research Center, Dokki, Cairo.

Selenium is an essential micronutrient for human and animal organisms. Five strains of locally isolated Saccharomyces cerevisiae were screened for their capabilities of accumulating large amounts of organically bound selenium. The effects of different levels of sodium selenite (50 up to 300 mgL-1) added at the beginning of fermentation time, on yeast biomass and organically bound selenium accumulation in the two selected highly potent strains (S. cerevisiae No. 5X and F-25), were investigated. It has been found that introduction of 250 mgL-1 sodium selenite in the culture medium added after 24h of growth in shaking flasks resulted in high organically bound selenium accumulation of 1497.1 µg g-1 dry biomass S. cerevisiae F-25. Under suitable batch fermentation conditions done in 500L pilot scale fermenter with S. cerevisiae F-25, high concentrations of organically bound selenium yeast cultures were yielded. To verify the viability and quality of high selenium yeast produced from different batches in this study compared to untreated control yeast, the amino acids profiles and invertase activities were investigated. As a result, pilot scale fermentation demonstrated slight variations in performances of the total amino acids concentrations and enzyme activities of high selenium yeast batches and control. Scanning electron microscopy (SEM) analysis was carried out to observe cell surface and shape variation of yeast cells produced from different batches of high selenium yeast and control. The accumulation of organically bound selenium in the cells demonstrated no significant effect on cells which had unimpaired shape with smooth surface. Energy dispersive X-ray (EDX) analysis was conducted for a comparative examination of selenium binding in the biomass cells of high selenium yeast batches and control. The results illustrated gradual decrease in sulfur (similar in chemical properties to selenium) content was concomitant with the increase of organically bound selenium in yeast cells as compared to control (with higher sulfur cells). These results clearly demonstrate enhanced production of organically bound selenium yeast in pilot scale batch fermentation and confirm that the process is reproducible.

 

 


8/9 PURIFICATION AND PROPERTIES OF UREASE FROM EGYPTIAN CABBAGE LEAVES (BRASSICA OLERACEA)

S.T. El-Sayed

Biochemistry Department, National Research Center, Dokki, Cairo, Egypt.

Urease level was quantitatively screened in some plants. Egyptian cabbage leaves belonging to family Cruciferae, have the highest urease content (193.3 U/g fresh leaves). To stabilize the solution form, 1.0 mM EDTA and 10% glycerol were routinely added to the enzyme solution during purification steps. Urease was purified from cabbage leaves through a simple reproducible method consisting of extraction, ammonium sulfate and acetone fractionation and sequential column chromatography (gel filtration) including Sephadex G-120 and Sephadex G-200. The enzyme was purified 23.4 fold with a recovery of 50.74 % and 663.6 U/mg protein. The electrophoretic behavior of the final preparation showed a single protein band, which proved its homogeneity. The molecular weight estimated by gel filtration on Sephadex G-200 was 140 KD. The optimum urease activity was at pH 5.0 to 7.0 and 26ºC with activation energy of 6.8 Kcal/mole. Hydrolysis of urea by cabbage urease was a linear function of enzyme concentration and urea concentration up to 50 mM/assay. It was also linear relation with time up to 25 min. The Km value for cabbage urease towards urea was 2.6 and 2.5 mM at pH 5.0 and 7.0 respectively. Heat stability studies showed the high stability of the enzyme activity. It was stable for 45 min up to 50 and 60ºC with only loss 13 and 29 % of its activity respectively. Fe3+, Hg2+, Co 2+, Cu 2+, Na + and Mg2+ inhibited cabbage urease activity with 100, 79, 77, 75, 70 and 52% inhibition respectively at 5.0 mM concentration. Urease activity was inhibited by 2x10-7 p-chloromercuribenzoate (PCMB) and lost 37 % of its activity.The laboratory determination of urea in some normal sera gave resemble results to that obtained by medical service unit in National Research Center. The prepared enzyme had good activity, stability and properties that offer potential for practical application in clinical medicine.

9/9 COMPARATIVE CHEMICAL AND BIOLOGICAL STUDIES OF THE VOLATILE OIL OF VARIOUS PARTS OFFOENICULUM   VULGAREMILL. ANDFOENICULUM DULCE DC. GROWING IN EGYPT.

F.M. Soliman, M.A. El-Sohly*, A.H. Shehata, A.E. Khaleel, S.M. Ezzat

and A.A. Sleem**

Pharmacognosy Department, Faculty of Pharmacy, Cairo University,Kasr Elainy street, Cairo 11562,Egypt.

*Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, Mississippi, MS. 38677 U.S.A.

**Pharmacology Department, National Research Center, Dokky, Giza, Egypt.

The volatile oil composition of various parts of Foeniculum vulgareMill. andFoeniculumdulce DC. (Fam. Apiaceae) was analyzed by GC/MS. The major component of the essential oils of all organs of F. vulgare Mill. except the root was methyl chavicol (estragole) (47.64 % - 79.41 %). Meanwhile, trans-anethole was found to be the major constituent in the essential oils of all organs of F. dulce DC. (39.07% - 81.06%) except the root. D-limonene was a major constituent in the oils of all organs of both plants (1.85% - 40.33 % in the oils F. vulgare Mill. and 2.82% - 27.20% in the oils of F. dulce DC.). F. vulgare Mill. provided oils with quite low amounts of fenchone. L- fenchone was present only in the essential oils of the fruit (3.9%) and flower (0.26%) and absent in the oils of the leaf, stem and root. On the other hand, fenchone was present in the essential oils of all organs of F. dulce DC. (0.1% - 11.1%). The composition of the essential oil of the root of both plants under examination differed from the composition of the essential oils of the other organs. The major components of the root oil of F. vulgare Mill. were dill apiole 56.80% followed by myristicin 19.57% and those of the root oil of F. dulce DC. were apiole 36.36%, myristicin 30.15% and dill apiole 10.29% . The essential oil of the leaves of both F. vulgare Mill. and F. dulce DC. showed the highest activity against Ehrlich ascites carcinoma cell line (100% inhibition of tumor cell viability at all tested concentrations). Both oils had an apparent inhibitory activity on the viability of the two human cell lines: U251(brain cancer cell line) and MCF 7(breast cancer cell line) cell lines . The essential oils of the total herb, fruits, flowers and leaves of both F. vulgare Mill. and F. dulce DC. showed a significant diuretic activity in rats without causing hypokalemia. The essential oils of the total herbs and the fruits of both plants demonstrated antioxidant properties comparable to vitamin E.

 

10/9 BIOLOGICAL PROPERTIES OF SAPONIN MIXTURES OF OREOPANAX RETICULATA HORT. AND OREOPANAX GUATEMALENSIS DECNE & BLANCH FAMILY ARALIACEAE

O.A.EL-Shabrawy, S.M.Abdel-Khalik*, F.R.Melek**, I.I. Mahmoud and M.H.Hetta**

Pharmacology Department, National Research Centre, Dokki, Cairo, Egypt.

*Pharmacognosy Department, Helwan University, Cairo, Egypt.

**Chemistry of Natural Products Department, National Research Center, Dokki, Cairo, Egypt.

The saponin mixtures (SM) of Oreopanax reticulata hort. And Oreopanax guatemalensis decne & blanch have been studied for their toxic, analgesic, antipyretic, anti-inflammatory and anti-ulcerogenic activities. Acute single oral doses up to 800 mg/kg. Showed no lethal effect, only sluggishness was observed with high doses. SM of O.reticulata hort. Showed more analgesic effects than that of O. guatemalensis decne & blanch and the antipyretic effect of both SM are approximately similar. SM of O. reticulata has more anti-inflammatory effect than O. guatemalensis. A significant antiulcerogenic effect was demonstrated by both SM.

11/9 PLASMID PROFILE ANALYSIS OF SOME PATHOGENIC MICROORGANISMS EXPOSED TO GAMMA IRRADIATION

H.A. Farrag

Lab. of Microbiology, Drug Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority,

P.O. Box 29 Nasr City, Cairo, Egypt.

Plasmid profile on agarose gel electrophoresis of seven pathogenic microorganisms were used in conjunction with biotyping and antibiogram to study the effect of 25 Gy gamma irradiation on bacterial infection in cancer patients. In some cases patients had cultures of Pseudomonas aeruginosa, Morganella morganii, Escherichia coli and Providencia rettgeri, from urine, belonged to the same biotype, but had different antibiograms and distinctly different plasmid profiles. In other instances, patients had cultures of Klebsiella pneumoniae from sputum or Pseudomonas aeruginosa and Serratia marcescens from blood belonged to different biotype, markedly different antibiograms but with identical or different number of plasmid bands after irradiation than before. A great problem after irradiation is the multiresistant strains to great number of antimicrobial drugs. Before irradiation the results showed that all tested isolates were resistant to two antibiotics, tobramycin (aminoglycosides) and tetracycline (tetracyclines). While, after irradiation all tested isolates were resistant to tobramycin, and tetracycline as well as 71.4% - 85.7% were resistant to 3 antibiotics, including gentamicin, kanamycin (aminoglycosides) and sulphamethoxazole trimethoprim (sulfonamide). Whereas, 71.4% were intermediate or resistant to cephalothin (cephalosporins, beta-lactam). Plasmid profile analysis after irradiation showed more extra plasmid bands than before irradiation with difference in the mloecular weight. The majority of the tested strains had 2 or more bands with molecular weight more than 3147bp. The use of plasmid profiles, either in conjunction with other methods of strain identification or by itself, is a powerful tool in providing an additional fingerprint of organisms in an   epidemiologic study.

 

12/9 PURIFICATION AND CHARACTERIZATION OF INULINASES FROM CYNARA SCOLYMUS AND PISUM SATIVUM WASTES FOR ENZYMATIC FRUCTOSE PRODUCTION

S.T. El-Sayed

Biochemistry Department, National Research Center,

Inulinases (EC 3.2.1.7) were purified up to electrophoretic homogeneity from Cynara scolymus (I) and Pisum sativum (II) wastes. They were highly purified by precipitation with ammonium sulfate followed by gel filtration on Sephadex G-120 column and two successive Sephadex G-200 columns. The inulinases (I) and (II) were purified 75.6 and 36 times with yield recovery of 22 and 24.6 %, respectively as compared to the crude preparation. The apparent molecular weights estimated by gel filtration on Sephadex G-200 were 75 and 66 KD for inulinase (I) and inulinase (II), respectively. Both inulinases (I) and (II) have optimum activity at 50ºC, with activation energy (Ea) of 3.39 and 14.21 Kcal/mole, respectively. Inulinase (I) showed maximum activity at pH range from 6.0 to 7.5, while inulinase (II) have optimum activity at pH 7.0. Inulinase (I) had maximum pH stability at range from 4.5 to 6.0, while inulinase (II) had maximum pH stability from 4.5 to 5.0. Inulinase (I) was fairly stable to heat up to 40ºC for 60 min with only 31 % loss of activity. But it lost 68 % of its activity when heated at 60ºC for 60 min. While inulinase (II) was heat stable up to 60ºC for 60 min with only loss 32 % of its activity. Thus, inulinase (II) is highly thermostable than inulinase (I). They gave ultraviolet spectra characteristic of protein. The purified enzymes liberated fructose as the only product from inulin, indicating an exo-mode of action (exoinulinase). They were completely substrate specific for inulin. The Km values for inulinases (I) and (II) toward inulin were found to be 0.33 and 0.52 %, respectively. They were devoid of b-D-glucosidase activity. The activities of inulinases (I) and (II) were inhibited by Hg2+, Mg2+, Cu2+ and Fe2+and were activated by EDTA. The prepared enzymes (I) and (II) were used for production of fructose by enzymatic hydrolysis of some vegetable tissues for medical and industrial applications.

13/9 PHYTOCHEMICAL AND BIOLOGICAL STUDY OF

PETROSELINUM SATIVUM (Hoffm) CULTIVATED IN EGYPT

(PART II)

O. Rashwan, M. Fouad*, S.M. Abd El-Wahab and G.H. Mahran

Dept. of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt

*Institute of Nutrition, Ministry of Health, Cairo, Egypt.

The ether extract of the aerial parts of Petroselinum sativum (Hoffm) afforded four furanocoumarins: xanthotoxin, isopimpinellin, bergapten and sphondin ; while that of the fruits yielded only bergapten. Sphondin was isolated for the first time from the plant. The pectin content of both samples was investigated and revealed the presence of galacturonic acid (76.84% and 76.31%) and arabinose (8.8% and 16.68%) as the major sugar components in the aerial parts and fruits respectively, in addition to rhamnose, xylose, galactose and glucose. Minerals and trace elements composition was determined, potassium (19,93 and 29.01%), calcium (16.32% and 26%) represented the major components in both aerial parts and fruits respectively. Iron, zinc, antimony and selenium were also detected.Biological investigation of the different extracts, of the aerial parts: total aqueous, petroleum ether (60-80oC), chloroform, ethyl acetate and alcohol was carried and showed that all extracts exhibit diuretic, natriuretic and kaliuretic activities, chloroform extract is the most effective. Also all the extracts showed inhibitory growth towards gram +ve and gram -ve bacteria and exhibited a significant degree of inhibition on the growth of Candida albicans, the alcoholic extract is the most potent. The total aqueous extract exhibited, in addition, significant antispasmodic activity.

14/9 THE DISTINCT ROLE OF INTERLEUKIN-4 AND INTERLEUKIN-13 IN EXPERIMENTAL MICE INFECTED WITH SCHISTOSOMA MANSONI: INTERLEUKIN-13 IS A HEPATIC PROFIBROTIC CYTOKINE

N.F. Bouzubar*, N.M. Al-Ajmi*, B.M. A. El-Falaha** A.A. Ramadan***

*Biochemistry, **Microbiology, ***Parasitology, Department of Medical Laboratory Technology, College of Health Sciences, PAAET, Kuwait.

In this study the availability of a mouse anti-interleukin-4 (anti IL-4) and anti-interleukin-13 (anti IL-13) monoclonal antibodies (mAbs) has permitted the intimate dissection of the direct role of IL-13 in a type-2 cytokine-mediated infection. Our study demonstrates that blocking of IL-13 from the Th-2 cytokine response to Schistosoma mansoni infection is beneficial to host survival. This enhanced prognosis correlates with a reduction in hepatic collagen deposition and indicates a novel role for IL-13 in the development of hepatic fibrosis. By contrast, the study has also showed that blocking of IL-4 results in very high mortality characterized by a breakdown in intestinal integrity and the development of endotoxemia, indicating that IL-4 as a protective cytokine in schistosome infection. The combined blocking of both IL-4 and IL-13 demonstrates that the positive effects resulting from the ablation of IL-13 were over-ridden by the detrimental pathology resulting from IL-4 blocking. In addition, combined cytokine blocking resulted in a phenotype that was considerably more severe and deleterious than that observed in the IL-4 blocking group. These results indicate the potential benefits of anti IL-13 as an antifibrinogenic cytokine, but also show the dangers of blocking both IL-13 and IL-4 concurrently.

15/9 REDUCTION OF MACROPROLACTIN INTERFERENCE IN HYPERPROLACTINEMIA BY PEG TREATMENT IN CLINICAL SAMPLES

N.F. Bouzubar*, H. A. Al-Mukhazim**, N.M. Al-Ajmi*

* Department of Medical Laboratory Technology, College of Health Sciences, PAAET, Kuwait.

**Hormones Laboratory, Sabah Hospital, Ministery of Health, Kuwait.

The influence of polyethylene glycol treatment on prolactin level in 72 samples with concentration range 263 to 7070 mU/l has been investigated. Treatment with 12.5% PEG caused 0 – 90% reduction in prolactin level. In 41 patients with high level of prolactin (> 600 mU/l) a reduction of 55 – 60% was detected. Five patients out of the 41 (12%) showed the effect of macroprolactin in the employed method, compared to 4 out of 31 (13%) with prolactin level below 600 mU/l. Macroprolactin in the latest group was not associated with any clinical condition.

 

16/9 EFFECT OF NOVEL COPPER COMPOUND (CU-BISDO) AND g-IRRADIATION ON COPPER AND ZINC CONCENTRATION, CERULOPLASMIN AND SUPEROXIDE DISMUTASE ACTIVITY, IN MICE LOADED WITH EHRLICHTUMOR.

E. Noaman and H. El-Kabany*

Radiobiology Research Department; and *Health Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority,

Cairo, Egypt;

Cu (II) bis (salicylate) dedecanol (Cu-BISDO) is a Copper complex compound that has been found effective as anticancer drug. Cu-BISDO was previously synthesized and tested by the authors for its antitumor activity. The present study was designed to investigate the effect of Cu-BISDO on the level of Copper and Zinc concentration Ceruloplasmin and Superoxide Dismutase (SOD) activity, in liver and tumor tissues and in plasma of mice bearing Ehrlich carcinoma tumor subjected or not to radiotherapy course. Results of this work indicate that the compound induced noticeable amelioration of SOD activity and ceruloplasmin concentration in blood and liver tissue. The increase in antioxidant activity may provide a protective role of this compound for healthy tissue and the increase in copper concentration in tumor tissue provides a mechanism for high selective role of Cu-BISDO against hypoxic cells in tumor tissues.

 

17/9 CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF LUFFA AEGYPTIACA MILLER GROWING IN EGYPT.

A.H. Shehata

Pharmacognosy Department, Faculty of Pharmacy, Cairo University,

Kasr El-Aini Street, Cairo, 11562, Egypt.

Three glycosides [named, kaempferol-3-o-rhamnoside(5), quercetin-3-o-rhamnoside(6) and B-sitosterol-B-D-glucoside(7)] and their aglycones [kaempferol(3), quercetin(4) and B-sitosterol(2)] were isolated and identified for the first time from the immature fruits of Luffa aegyptiaca Miller under investigation. In addition, B-amyrin(1) was also isolated. the structures of these isolates have been established on the basis of physicochemical data, UV spectral data, 1H-NMR, 13C-NMR and EI-mass spectrometry. The flavonoidal contents of the fruits were determined adopting TLC scanning assay of the aglycones. The recoveries were 99.69-99.52 with S.D. of + 1.161 - +1.07. The saponin content was found to be 0.32% w/w. Nutritional evaluation of the fruits revealed that vitamin C amounted to 6.49 mg/kg and that potassium and iron are the major elements. the crude saponin of the fruits exerted a potent anti-ulcerogenic effect (99%) on ethanol-induced ulcerogenic rats; while the alcoholic extract showed antihyperglycemic effect on alloxan-induced diabetic rats. The aqueous extract produces an elevation in the serum glucose level in normal and diabetic rats.

18/9 Iridoids and Phenylethanoids of Penstemon gentianoides Regel. And their anti microbial activity

A.A. El-Hela and S.K. Ismail.

Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt

Two iridoids glucosides (2’ -O– Foliamenthoyl plantarenaloside, geniposide and one phenylethanoid (acetoside) were isolated From the herb of Penstemon gentianoides Regel. by fractionation of the n- butanol extract using a polyamide and silica gel columns. The identity of the isolated compounds were established by chromatographic method (COTLC), spectral data (1H, 13CNMR and MS) and by direct comparison with authentic substances. Some of the isolated compounds were found to possess anti microbial activities towards different microorganisms.

19/9 PROGNOSTIC FACTORS IN AGGRESSIVE BASAL CELL CARCINOMA OF THE SKIN

H.A.H. Gadalla*, M.G. Mustafa, H.S.M. Atta, S. Osman# and A. El-Shandaweely*

Departments of Pathology and Surgery, Faculties of Medicine, Al-Azhar*, Assiut and South Valley# Universities

Basal cell carcinoma (BCC) represents one of the most common cutaneous cancers in human. Although it generally has a relatively benign course, some cases of BCCs have aggressive behavior evidenced by rapid infiltration of the deeper structures and even distant (often lymph nodal) metastasis. Therefore there is a need to find reliable prognostic indicators that correlate with outcome and may predict patients at high risk for BCC recurrence and metastasis. Study of cell proliferation indices and tumour neovascularization may be helpful in this regard. In the present study, 17 cases of non-aggressive BCC (BCC1) and 18 cases of aggressive BCC (BCC2), selected by clinical and histological parameters were investigated for nucleolar organizer region (NOR) status and tumour angiogenesis. NORs were evaluated via the argyrophilic staining of interphase nucleus technique. Tumour angiogenesis was expressed as microvessel density, highlighted by immunohistochemical staining for factor VIII-related antigen. The mean number of AgNORs per nucleus was significantly higher in BCC2 (7.7± 1.89) than in BCC1 (3.31±0.32) (p value was <0.0001). The study also revealed a statistically significant difference in angiogenic count between BCC1 and BCC2 (16.02±1.19 and 30.24±2.27 respectively; p value was <0.0001). Thus the results of the present study seem to establish a correlation between each of NORs count and tumour neovascularization and the clinicobiological parameters of aggressiveness in BCC.

20/9 STUDY OF CARDIOVASCULAR DISEASES IN DIABETIC PATIETNS

S.A. Saddik, O. El-Ahmady, L. Nabil

Biochemistry Department, Faculty of Pharmacy, Al-Azhar University,

Cairo, Egypt.

Excess coronary events continue at a high rate in the diabetic individual despite the use of effective hypoglycemic agents. A number of pathogenic possibilities deserve greater attention, so the current work has been carried out to study the incidence of developing coronary heart disease between diabetic and non-diabetics as well as between different types of diabetes, non insulin dependent diabetics mellitus (NIDDM) and non-treated diabetics. Regarding predisposing risk factors such as dyslipidemia, insulin resistance, hypertension, atherosclerosis all were interrelated to diabetes. This study was done on 129 subjects male and female, with the same average age and socioeconomic state, divided into three main groups. First, apparently healthy control group (n = 32). The second group, diabetic group which was subdivided into two subgroups, diabetic non-treated, and non-insulin dependent diabetes mellitus (NIDDM), (n = 58). The third group, complicated cardiovascular group which was divided into two subgroups, complicated cardiovascular NIDDM and cardiovascular non-diabetic subgroup (n = 39). The biochemical parameters measured in all subjects were glucose, insulin, aldosterone, CK-MB, AST, triglycerides, total cholesterol, HDL-cholesterol, LDL-cholesterol. Lastly we calculated two risk. Factors RI total cholesterol/HDL-cholesterol ratio and RII L DL-c/HDL-c ratio. We concluded that normalization of blood lipids should be an important feature of diabetes control. Fasting insulin level are used for measurement of insulin resistance. Dyslipidemia are common and powerful risk for the development of coronary disease. Intensive glycaemic control seems to delay the onset and slow the progression of coronary heart diseases. Improvement in lipid and lipoproteins profile enhanced the diabetic subject ability to avoid CHD.

 

21/9 PHARMACOKINETIC DISPOSITION OF MELOXICAM IN MALE RABBIT PLASMA

H.M.M. Arafa, A.M. Mansour and A.B. Abdel-Naim*

1Department of Pharmacology and Toxicology, Faculty of Pharmacy,

Al-Azhar University

2Department of Pharmacology and Toxicology, Faculty of Pharmacy,

Ain Shams University

The pharmacokinetic profile of meloxicam in male New Zealand white rabbits was investigated following a bolus i.v. injection of 10 mg/kg body weight to elucidate whether or not meloxicam has the same kinetic profile as in other previously investigated species including man. The drug was administered in phosphate buffered saline (pH 8) in a dosing volume of 10 mg/ml via the marginal ear vein. Blood samples were withdrawn from the marginal vein of the other ear at 5, 10, 15, 30 min and 1, 2, 4, 6, 8, 12 and 24 h following drug administration. Plasma samples were analyzed using HPLC. Plasma level of meloxicam apparently fell down relatively rapid during the first 4 h, ensuring a rapid distribution pattern; the apparent volume of distribution was 2.57 l/kg. The drug had a biphasic manner of elimination. The first phase was shorter and relatively sharp (4-8 h) and the other one was longer (8-24 h) and slower. Non-compartmental analysis of meloxicam revealed that, the drug had a very short terminal elimination half-life (t1/2; 2.34 h), as well as a relatively high total body clearance (Cltot; 0.84 l/h/kg) that may account for the relatively low total body burden (AUC0-¥; 13431.41 ng.h/ml) compared to values previously reported for other animal species. Simulation of plasma time profile revealed that the drug could fit a 3-compartment kinetic profile. In conclusion, meloxicam had a kinetic profile different from that previously reported for humans. Meloxicam had a total body burden less than in other animal species. However, like other species tested so far, meloxicam had a biphasic elimination pattern, besides the drug circulated in rabbit plasma as the parent compound during the investigated time course.

22/9 ERYSIPELO-CELLULITIS; A PROPLEM

M.M. Tawfik*, A. Abdel-Latif*, S.Z. El-Sayed**, A.A. Shalaby***,

A.G. El-Sheity**, R.H. Mansour** and R.R. Bedir****.

Tropical Med. Department, Cairo University*. Tropical Med. **, Microbiology***, and Dermatology****, Department, Faculty of Medicine, Al-Azhar University.

Erysipelas is an acute infection of epidermal surface of the skin typically caused by group A beta-hemolytic streptococci. Many patients suffer from this disease and its complications. Through one year (from October 2000 to September 2001), 339 patients were admitted to Imbaba Fever Hospital with symptoms and signs of erysipelas and/or cellulites. The disease is common in age group > 50 years (39.23%), female (52.51%), rural patients (61.65%), lower limbs (93.50%), and diabetic patients (47.20%). The isolated organism is group A beta-hemolytic Streptococci (35.39%) alone or associated with Staphylococcus aureus (43.65% of cases). Penicillin, cephalosporins, quinolones are highly effective therapy. The duration of therapy is from 15 to 25 days. The recurrence of the illness and the skin disfigurement are the main problems of erysipelas.  

 

 

23/9 CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OIL OF HELICHRYSUM

CONGLOBATUM STEUD.

M.A. Koheil

Department of Pharmacognosy, Faculty of Pharmacy,

Cairo University, Kasr El Aini, Egypt.

The chemical composition of the essential oil obtained from the aerial parts of Helichrysum conglobatum Steud. was analysed by GC/MS. Among the forty identified constituents representing 90.26 % of the total oil, β-myrecene , trans-β ocimene, geraniol, camphor and geranyl acetate were the major components. Furthermore, it was found that the oil exhibited significant antibacterial activity against Gram +ve bacteria.

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