Vol. 6, July, 1999.

cialis ou tadacip 1/6 TREATMENT FOR CLINICAL ENDOMETRITIS WITH GARLIC EXTRACT INFUSION IN DAIRY COWS

H.K. Abd El-Latif and *M.A. El Azab

Department of Microbiology, Faculty of Pharmacy, Zagazig University

* Department of Obstetric Gynecology Faculty of Veterinary Medicine, University of

Zagazig, Egypt

Antibacterial efficacy of garlic extract was studied in vitro and in vivo. This was judged on the basis of priligy vorzeitige ejakulation in vitro inhibition of microbial growth of isolated microorganisms on blood agar and the recovery of clinical cases ofendometritis by intrauterine infusion of garlic extract. Bacteriological samples before and after treatment were taken from 15 cows affected with 2nd and 3rd degrees ofendometritis. The initial bacterial counts ranged from l(f to JO12 Colony forming units/ml (cfu/ml) before treatment, were markedly reduced after garlic treatment to between 1O3 and 104 cfu/ml. For MBC determination, blood agar media with different concentrations of garlic extract ranging from 2 to 8% v/v were inoculated with isolated bacterial strain and subcultures were made from those having no growth. Concentration of 8% v/v killed all isolated strains. The pH value of garlic extract was 5.8 after incubation at 37 "C for one hour. The bactericidal activity of garlic against E. coli was compared with that of oxytetracycline, the drug of choice in treatment of endometritis The in vivo investigation disclosed that all cows having 2 degree endometritis responded well after single intrauterine infusion of garlic extract. All cows showing 3rd degree endometritis required two infusions of the garlic extract for complete clinical recovery.

c quoi viagra 2/6 CRUDE OIL BIODEGRADATION BY ITS OWN NATURAL

cialis et sep BACTERIAL FLORA UNDER DIFFERENT ENVIRONMENTAL

cialis ou viagra qual o melhor AND NUTRITIONAL CONDITIONS

N.M. Atef, N.M. Morsi and M.E. Mohamed Botany Department, Faculty of Science, Cairo University, Giza, Egypt

The biodegradation of two light crude oil wells (land and marine) in Red Sea area by its own bacterial microflora was studied under different environmental and nutritional factors. Oil biodegradation process was enhanced by aeration, moderate temperature, salinity (5%), urea, nitrogen/phosphorus ratio, iron (2.5 u mol), emulsifying agent and increased inoculum. Under all optimal conditions, the mixed bacterial culture consumed two thirds of the added oil in one week. Compositional variations with respect to C-, H-, N-, S-, P-contents and C/H ratio of the recovered oil were observed. Four bacterial genera naturally inhabiting the crude oil were isolated and characterized as Micrococcus, Bacillus, Corynebacterium and Pseudomonas.

vente de cialis à paris 3/6 STIMULATION OF CELLULOLYTIC AND PROTEOLYTIC PRODUCTIVITY BY STREPTOMYCES CEARULEUS

M.S. Khalil

Botany Department, Faculty of Science, Cairo University, Giza, Egypt.

A strain of viagra mal de tete Streptomyces cearuleus, a producer of cellulose and protease enzyme was selected to study the productivity of these enzymes under different nutritional and environmental conditions. This strain, when grown onperacetic acid-treated wheat bran, the growth of the organism is enhanced and favoured better celMolytic andproteolytic activities in the extracellular supernatant. The activity of both enzymes are enhanced when the starch in the basic medium was replaced with 8g/L treated wheat bran. It was found that 0.8% treated wheat bran and 0.1M citrate-phosphate buffer at constant pH (4.6), incubated for 7 days at 35°C are the optimum conditions for more productivity ofbiomass and the studied enzyme activities.

prise de viagra combien de temps avant 4/6 STUDIES ON SOME RELATIONSHIPS BETWEEN CERTAIN histoire du viagra SPECIES OF THE RANUNCULACEAE

A.I.I. Aboel Atta, E.A.Kamel, M.H.A. Loutfy, A.A.A. El-Mashad

Biological Sciences and Geology Department, Faculty of Education,

Ain Shams University, Roxy, Cairo, Egypt.

Morphological features (macromorphological characters), seed coat scanning (micromorphological characters), chromosome morphology (karyotype) and seed proteins electrophoresis were investigated to study the taxonomic relationships in the ranunculaceae. the ntsys-pc was used to study the relationships within the family, fifty-five morphological characters, 56 attributes from seed coat scan and 37 attributes obtained from protein analysis were used, five sets of classification have been suggested; the first employing the morphological characters, the second using the seed coat scan attributes, the third using the seed storage proteins attributes, the forth using the combined data of the morphological features and seed coat morphology and the last one using all characters (morphology, seed coat morphology and seed storage proteins profiles), these phenetic classifications were discussed in relation to the cytological features.

comparaison levitra et viagra 5/6 COMPATIBLE SOLUTES IN SALT - STRESSED FUNGI

M.S. Khalil

Department of Botany, Faculty of Science, Cairo University, Giza, Egypt

Two fungal organisms: Rhizoctonia solani and Sclerotium rolfsii were used to study the effect of different salinity stresses (-0.32 Mpa; -0.48 Mpa and -0.64 Mpa) on growth, ions content and polyols accumulation. The results revealed an obvious difference in the fresh and dry weights between control and treated samples, that was less apparent in Rhizoctonia than Sclerotium.The relative water content of either fungi was hardly affected by the different salinity levels. Both organisms grown under saline conditions accumulated large quantities ofNa, K and Cl; Rhizoctonia accumulated twice as much sodium and more than twice potassium than Sclerotium whereas their Cl content was almost similar. Inspite of this difference the Na/ K ratio was not affected. Glycerol, mannitol, erythritol and arabitol are compatible solutes produced by fungi in response to salt stress , to control the steady state osmotic potential of the medium . Erythritol is the predominant compatible cytoplasmic solute.

6/6 EFFECT OF HEAT SHOCK ON PROTEIN PROFILE AND

ELECTROLYTE LEAKAGE OF SIMMONDSIA CHINENSIS L.

(JOJOBA) ROOTS CULTURED 77V VITRO

A.H. Nassar

Department of Botany, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt

Heat shock of living tissues induces the synthesis of a unique group of proteins, heat shock proteins (HSPs). The response of jojoba roots cultured in vitro to two elevated temperatures, 40 and 52 C, for 20 min. and one hour has been investigated. Accumulation of significant levels of HSPs in heat shocked root cultures compared with the control, has been reported using SDS-polyacrylamide gel electrophoresis. Exposure of cultures to 4CrC induced the production of three new HSPs with molecular weights of 68, 60 and 9 KD, while exposing to 52°C induced the formation of four new HSPs with molecular weights of 68, 60, 30 and 9 KD. Most of the proteins normally synthesized at 28° C continued to be produced at the two elevated temperatures but at higher levels. Electrolyte leakage test for viability showed an injury between 12 and 39%, when cultures were exposed to elevated temperatures. The results obtained in the present study showed relatively high thermo-tolerance of jojoba plants.

7/6 CHARACTERIZATION OF BACTERIOCINS FROM TWO STRAINS OF STREPTOMYCETES

G. Enan, E.A. El-Sherbiny, M. Abo El-Hawa* and S. Abd El-Shafi Botany Department, Faculty of Science, Zagazig University, Zagazig, Egypt.

The Streptomyces halstedil (25) and Streptomyces avellaneus (Z7) strains isolated from Egyptian soil appeared to be able to inhibit other strains of Genus Streptomyces including the virulent Streptomyces scabies strain. Their inhibitory substances were resistant to heat, sensitive to proteolytic enzymes and were active on closely related species. Consequently, they could be characterized as bacteriocins designated streptocin (25) and avellacin (27) respectively. Both of them were resistant to organic solvents and lipase, indicating the absence oflipid moieties in their molecules. Streptocin (25) appeared to contain glucidic moieties in its molecule, but avellacin (21) not do so. Bacteriocins production were correlated with growth of the producer strains. The Bactericidal action of avellacin (27) was more pronounced on sensitive streptomycetes than streptocin (25).

8/6 LEVELS OF ENDOTOXINS AND MICROBIAL CONTENT OF DUST STORMS IN KUWAIT

M.Y. Al-Gounaim and A. Diab

College of Basic Education, P.O.Box 34053 Idailya, Kuwait

Dust samples were collected from the atmosphere of Kuwait during the dusty periods of the year 1994, 1995, 1997 were microbiologicaly examined. Dust samples collected during the year 1997, as compared to other samples collected during 1994, 1995 were rich in thermophilic bacteria and thermophilic actinomyces. While samples collected during the year 1994 were rich in mesophilic bacteria, mesophilic actinomycetes and fungi. Out of 306 bacterial cultures tested, 73.5% were starch hydrolysers, 20.3% were protein decomposers and 6.2% were lipase producers. Fifty bacterial isolates of gram negative rods were isolated from the different dust samples, of which 48 isolates could be identified into six genera: Enterobacter, (7 strains), Acinetobacter (8 strains), Aeromonas (6 strains), Flavobacterium (17 strains), Pseudomonas (8 strains) and Moraxilla (1 strain). One of the important contents of dust particles collected during dusty periods was the high levels ofendotoxins (625-2500 EU/g), Bacillus cere us and Clostridium perfringeus. A total of 152 cultures of mesophilic actinomycetes (MA) were identified into four genera: Streptomyces (73.0%), Nocardia (14.5%), Streptosporangium and Streptoverticillum (8.6% each). The 133 isolates of the thermophilic and/or thermotolerent actinomycets were identified into four genera: Thermoactinomyces (41.4%), Saccharomonospora (33.4%), Streptomyces (19.5%) and Pseudonocardia (5.3%). Thermoactinomyces included three species of which T. vulgaris was domnant (70.9%), it was isolated from all dust samples. Saccharomonospora and Pseudonocardia each included one species, S. viridis and P. thermophila. On the other hand Streptomyces included four species, of which S. thermovulgaris was highly frequent (46.2%).

9/6 PURIFICATION AND SOME PROPERTIES OF PECTIN METHYL ESTERASE FROM STREPTOMYCES CITREUS

E.Y. Tohamy

Botany Department, Faculty of Science, Zagazig University.

Purification of pectin methyl esterase enzyme (PME) of Streptomyces citreus was carried out by Gel Alteration using sephacryl S-200 column chromatography and agarose gel electrophoresis. The purification process resulted in pure protein with specific activity of 1.84 units/mg protein, thus the purification process increased the concentration up to 70.3 fold. The molecular weight of pure pectin methyl esterase was 110.000 dalton as determined by polyacrylamide gel electrophoresis. The purified pectin methyl esterase showed the highest activity at pH 4.4 and 40oC after an incubation period 30 minutes. Also the enzyme was activated by Zn+, A/g+ , Co+ , Cu+2, Fe+2 andMn*2, and inhibited by Pb\ Ca+2 andCcT2.

10/6 PERIODICAL FLUCTUATIONS OF PHYLLOPLANE FUNGI OF ZEA MAYS AND EVALUATION OF THEIR POTENTIALITIES IN CELL WALL DEGRADATION

S.A. Ouf, T.M.A. Abdel-Rahman, M.I.A. Ali and K.F. El-Yasergy Botany Department, Faculty of Science, Cairo University

A total of 6242 colonies/100 cm2 related to 17 genera were isolated throughout the cultivation period from the leaf surface ofZea mays. The bulk of the count was obtained using the washing technique, followed by the maceration and impression techniques. The isolated mycopopulation by the three methods gradually increased with age of plant and was peaked after 90 days reaching 1455 colonies/100 cm2. Lower isolate counts were recorded at the youngest and oldest plant ages. According to the percent of total population collected by the three techniques, the isolated genera could be arranged in descending order as follow: Aspergillus > Paecilomyces > Alternaria > Fusarium > Curvularia. The cell wall degradability assays indicated that all tested species had been able to degrade the carbohydrate and lignin constituents in the cell wall preparations ofZ. mays releasing reducing sugars and phenolic acids, respectively in the growth media and reaction mixtures. The power of degradation differed from species to another depending upon the amount and activity of the induced cell wall degradation exoenzymes. Fusarium moniliforme was the most potent species in degradation and was followed by Aspergillus niger, Curvularia geniculata and Alternaria hunicol.

11/6 AMELIORATION OF NaCl STRESS BY BORON IN PISUM SA TIVUM SEEDLINGS

A.A.A. El-Mashad and E.A. Kamel

Department of Biological Sciences and Geology, Faculty of Education, Ain Shams

University, Cairo, Egypt.

The activity levels of the endogenous auxins and growth inhibitors, gibberellins and cytokinins were determined in the extracts of seedlings ofPisum sativum after 6 days of germination. Application of the low concentration of salt (50 mMNaCl) or boron (10 mg/L) resulted in an increase in the levels of endogenous growth regulating substances. 150 mM NaCl induced opposite changes in these hormonal activities. The combination of the boron and the salt caused variable changes in the phytohormone activities. The high level of NaCl (150 mM) decreased the length of the radicle & plumule and the fresh & dry weights of seedlings, whereas boron increased these parameters. Mitotic index and some abnormalities were studied in the treated plants. The SDS-PAGE banding pattern of Pisum sativum seedlings extracted in Tris-Glycine and Tris-HCl, showed that the low concentration of NaCl increased the number of protein bands, while the high concentration decreased this number. The combination of boron with NaCl caused an increasing in total number of protein bands compared with the total number recorded by using (150 mMNaCl) alone. In addition, both concentrations of NaCl (50 and 150 mM) induced de novo synthesis of a salt responsive protein of molecular weight 29.0 KD. Also, boron (JO mg/L), NaCl (50 mM) and NaCl (150 mM) + boron (10 mg/L) induced the de novo synthesis of a protein with a molecular weight; 62.0 KD using Tris-HCl buffer. Moreover, a low molecular weight salt responsive protein (MW 32.1 KD) appeared in response to all treatments using Tris-Glycine buffer. However, two salt responsive proteins of molecular weights 62.0 and 65.3 KD were appeared in Pisum sativum seedlings treated with 50 mM of NaCl using the same buffer.

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