Vol. 7, January, 2000.

viagra et anticoagulant 1/7 CHROMOSOMAL ALTERATIONS IN MITOTIC DIVISION INDUCED BY SELENIUM POLLUTANTS

Z.A. Abo-Elkhier And Eman Abd El-Shafy

Department of Botany, Faculty of Science, Al-Azhar University (for girls),

Cairo, Egypt.

The cytological effect of selenium, in the form of sodium selenite solutions, was evaluated using Viciafaba seeds. Tip cells of roots arising from dry and soaked seed treatments exhibited a pronounced percentage of chromosomal aberrations. The mitotic index was slightly affected. Growing Vicia faba seedlings (18-days old) in soils polluted with Na selenite resulted in a decrease in pollen grain viability in comparison with the control. Light and scanning electron microscope observations showed the presence of defected pollen grains due to the deleterious effect ofNa selenite on Viciafaba plants.

vill du köpa viagra 2/7 PRODUCTION, PURIFICATION AND PROPERTIES OF pharmacie en ligne cialis 10mg POLYGALACTURONASE FROM BACILLUS CEREUS, I-TAT1 ISOLATED FROM THE MICROFLORA OF TUT-ANKHAMEN

difference cialis viagra TOMB

M.S. Ammar, H.H. El-Hendawy*, H.M. El-Berry and R.A. Bayoumi

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.

*Botany Department, Faculty of Science, Helwan University, Cairo, Egypt.

Bacillus cereus, I-TAT1, isolated from Tut Ankhamen Tomb (TAT) was found to produce polygalacturonase (PG) in a basal salt medium supplemented with pectin as a sole carbon source. The maximum level of extracellular PG activity was obtained on 100 ml of basal salt medium supplemented with 0.5% pectin and incubated for 48 h at 30°C. 1.48 x!0n cfu ml prepared from 24 h-old culture was the most favorable inoculum size and the optimum pH value was 6. All ingredients of the production medium, except KHiPO^ proved to be necessary for maximum PG production. Galactose was the highest inducer carbon source, whereas ammonium citrate was the most suitable inorganic nitrogen source, and tryptophan was the best amino acid. The enzyme was purified to 54.6 fold by precipitation at 80% (NH4)2804 before application to sephadex G-200 column chromatography. The specific activity was 31473.0 unit/mg protein. The activity of the purified enzyme increased gradually with the increase of time for 24 h. The pH and temperature optima were 6 and 30°C and the optimum pectin concentration was 0.1%. The enzyme exhibited thermal stability at 25°C and 30°C. It was also stable at pH 4 which refers to its possible applicable value in fruit juice industries.

cialis aspirine 3/7 EFFECT OF ANTRACOL AND BAYLETON FUNGICIDES acheter du viagra a paris sans ordonnance ON THE MEIOTIC DIVISION AND ON THE DIFFERENT cialis à bas prix PROTEIN FRACTIONS

Z.A. Abo-Elkhier

Botany Department, Faculty of Science, Al-Azhar University (for girls),

Cairo, Egypt.

The cytological effects of the two fungicides antracol and bayleton on Vicia faba plants have been investigated. Their effects on the harvested seedpprotein fractions legumin, albumin andovalbumin have also been considered. Both fungicides induced marked chromosomal irregularities in pollen mother cells (PMCs) of the tested plants. Analysis of total protein content of seeds produced from V. faba plants sprayed with antracol and bayleton showed bands with molecular weights different from the used marker. A variation in the amount of legumin, albumin and ovalbumin was also recorded.

prendre du viagra à 17 ans 4/7 CHROMOSOMAL STUDIES ON SOME SPECIES IN THE buying viagra in france RANUNCULACEAE

E.A. Kamel

Biological Sciences and Geology Department, Faculty of Education, Ain Shams

University, Cairo, Egypt.

A karyological study of 15 species of the family Ranunculaceae is presented. This study deals with chromosome number and karyotype features, i.e. chromosome length (CL) and karyotype asymmetry expressed as arm ratio (AR), total form percent (TF%), intrachromosomal asymmetry index (At) and interchromosomal asymmetry index

forum pour acheter du cialis 5/7 BIOSYNTHESIS AND SOME PROPERTIES OF POLYGALACTURONASE ENZYME FROM Aspergillus niger

S.A. Khalaf

Botany Department, Faculty of Science, Zagazig University, Egypt.

A local isolate of Aspergillus niger (strain 2) produced high amount of polygalacturonase .enzymes (PG and endo-PG) when grown in liquid medium containing apple pectin as sole carbon source among 16 tested Aspergillus isolates. This isolate of A. niger 82 isolated from apple-processing wastes, gave the highest activity of PG (3.90 U/ml) and endo-PG (4.25 U/ml) under optimum growth conditions. The maximum production ofPG (6.12 U/ml) and endo-PG (12.85 U/ml) was obtained when sugar-beet was used as sole carbon source. The crude polygalacturonase enzyme was stable at sufficiently high temperatures and low pH values.

6/7 DISTURBANCE OF CERTAIN METABOLIC PATHWAYS OF ASPERGILLUS FLA VUS DUE TO COBALT TREATMENT

H.G. Soliman

Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.

Aspergillus flavus an isolate from Sharm El-sheikh area, Sinai, Egypt grows on Czapek 's liquid medium supplemented with cobalt chloride concentrations up to lO.OOOppm. The mycelial growth was decreased with increase concentrations of Cod? and it was completely inhibited at 15, OOOppm while the protein contents and the protein bands were increased in presence of CoCh. Cystine, asparagine and proline were detected in the mycelial extract. Tryptophane, serine and alanine were in 10, OOOppm CoCh while glutamic, threonine and tyrosine were detected in control, 2500 and 5OOOppm CoCl2. Valine and arginine were in 5OOOppm CoC^. Phenylalanine appeared in control, 2500 and lO.OOOppm CoCh. The organic acids production was increased with increase of Cod? concentrations. Gluconic acid was predominant in all treatments, a-oxoglutaric and benzoic acids were detected only in SOOOppm & JO.OOOppm CoCh while fumaric was detected in SOOOppm only. Malonic acid was detected in 10, OOOppm and succinic acid was in 2500ppm C0C7?.

7/7 ENZYMATIC cDNA DETECTION AND IDENTIFICATION OF TWO EGYPTIAN CITRUS VIROID ISOLATES

A.B. Barakat, A.M. Sabek* and H.A. Arnin**

Microbiology Department, Faculty of Science, Ain Shams Univ.

*,**Plant Pathology Inst. Virus Res. Department, ARC.

The total RNA was extracted from infected mandarin and citron trees grown in El-Kalubeia and Giza governorates, Egypt by guanidium thiocyanate method. The full-length cDNA was prepared from purified RNA of the two Egyptian isolates by two synthetic oligodeoxyribonucleotide primers. DNA primers (20-19 nucleotides in length) specific for citrus exocortis viroid (CEVd) and citrus cachexia viroid (CCaVd) sequence were used for DNA synthesis and specific amplification of CEVd and CCaVd respectively. Analysis of RT-PCR by gel electrophoresis or by hybridization indicated their sensitivity and provides information regarding viroid detection without requiring large samples. RT-PCR assays were developed for the detection and identification of CEVd and CCaVd from nucleic acid extracts of infected citrus tissue. The size of the major RT-PCR product from CEVd-E infected tissue was the same as the full-length CEVd (375 bp). The size of the major RT-PCR product from CCaVd-E infected tissue was approximately 300 bp. These products did not detect in amplified extraction of uninfected tissue. The validation of RT-PCR amplified products of the two isolates was performed by southern, dot and squash blot hybridization. By squash and dot blot hybridization CEVd and CCaVd were detected directly in their natural tissue. Dot blot, however, seems to be more sensitive than squash blot since it could detect 2 pg and 100 pgofCCaVd-cDNA and CEVd-cDNA respectively. The detection of RT-PCR products of CEVd-E and CCaVd^E by southern and dot blot assays revealed the high sensitivity of such techniques comparing with agarose gel electrophoresis.

8/7 CHARACTERIZATION OF DIFFERENT CITRUS EXOCORTIS AND CACHEXIA VIROID ISOLATES BY SINGLE STRAND CONFORMATION POLYMORPHISM (SSCP)

A.M. Sabek, A.B. Barakat* and H.A. Amin**

**Plant Pathology Inst. Virus Res. Department, ARC.

*Microbiology Department, Faculty of Science, Ain Shams Univ.

Members of citrus exocortis viroid (CEVd) and hop stunt viroid (HSVd) related group II (CVHb= CCaVd, CVlla) has been distinguished by their biological properties in mandarin mother tree and Etrog Citron respectively. All viroid isolates were reverse transcribed and subsequently amplified in vitro from total nucleic acid extracts. The amplified products were subsequently sequenced using three distinct primers, one set for CEVd and two for HSV group 11. Cloning of viroid isolates was validated by plasmid minipreparation followed by EcoRl endonuclease digestion of recombinant plasmid then screened by PCR. Six clones of CEVd-E (Egypt), ten clones of CEVd-P (Portugal) and three clones ofCEVd-F (France), as well as, eight clones of CCaVd-E were identified. All clones had found to contain the inserted cDNA with the expected molecular weights. The RT-PCR of amplified products of the full length of the three CEVd-cDNA isolates (E, P &F) and the HSVd-cDNA clones showed 375 bp and 300 bp (nucleotides) respectively. The relationship between the different single strand conformation polymorphism (SSCP) profiles observed among clones and the variation in their nucleotide sequences should be confirmed by sequence analysis. The SSCP analysis of the three CEVd-cDNA isolates revealed different profile patterns among them, in addition to its ability to detect a number of variants within a single isolate. Similarly, HSVd-variants treated with two specific primers (HSVd-variant primer and HSVd-prototype primer) showed different patterns. By using this technique, the discrimination between DMA-Fragments of the same size containing any sequence variants was possible to detect.

9/7 RIBOFLAVIN PRODUCTION BY ASPERGILL US TERREUS USING WATER-HYACINTH HYDROLYSATE

M.A.   Foaad and A.F. Afifi,

Botany Departement, Faculty of Education, Ain Shams University, Roxy, Heliopolis, Cairo, Egypt.

Lignocellulosic hydrolysate of waterhyacinth was used as a sole carbon source for riboflavin production by Aspergillus terreus .The results showed that the highest amount of the riboflavin occurred in the fermentation medium composed of 50% hydrolysate and 50 % of the basal medium. The composition of the basal medium was as follows (g/l): glucose, 10; aspargine,5.98; KH2PO4, 2; K2HPO4, 2; MgSO4.7H2O, 1.0; The optimum pH of the medium was at 6.5andthe temperature was 30° C.

10/7 VARIATION IN CATTLE BEHAVIOUR DUE TO PREGNANCY AND PRODUCTION RANKING

I. S. Meneeh* and H. Samaha**

*Dept. of Anim. Husb. Fac. Vet. Med. Alex. Univ.

**Dept. of Hyg. and Zoonoses Fac. Vet. Med. Alex. Univ

Animals utilized in this study were 20 females, 10 pregnant heifers and JO pregnant cows between the 3rd and 9th months of pregnancy belonging to El-Shazly dairy farm between 2 and 6 years old. Moreover, dairy cows were divided into three grades according to their milk production, high (more than 20 kg/day), medium (14-20 kg/day) and low producers (less than 14 kg/day). Results of this study are summarized in two compact Tables (1 and 2). Least square analysis of data concerned observations of dairy cows during pregnancy and lactation revealed that pregnant cows showed longer feeding, rumination, standing, and lying times than the pregnant heifers. Moreover, they found to have higher self licking, and licking others while they have lowered movement activities than pregnant heifers. Generally, with the advance of pregnancy there was gradual increase in feeding time and movement activities. On the other hand, high yielding cows were found to spend more time feeding, less time rumination, more time standing but less time lying, less frequencies of walking and running. Moreover they performed higher body care activities and higher social encounters than medium or low producers.

11/7 IS THE CASTRATION NECESSARY FOR THE PERFORMANCE OF LAMBS?

U.E. Mahrous and I. S. Meneeh

Animal Husb. Dept, Fac. Vet. Med., Alex. Univ.

28 lambs were allotted into five groups, control group, two groups were subjected to castration at early age (21 day) and two groups were subjected to castration at later age (56 day) by Burdizzo and surgery. After slaughtering at average 239 day, skinning and evisceration took place and the internal organs and body cavity fat (omental, intestinal, pelvic and kidney fat) were removed and weighed. After chilling for 24 hr., the carcass of each lambs was weighed and divided into two halves, the left side was weighed and the right side was cut into eight retail joins for evaluation of carcass composition, moreover, the components of the loin cut (meat, fat and bone) were separated and weighed. Instrumental colour (L, a and b values) ofL. dorsii surface by Chromameters and free fatty acids in perinepheric, subcutaneous and L dorsii fat by chemical analysis were estimated. The lambs that castrated by Burdizzo clamp either in early or later age had higher body weight and weight gain than control ones, while, that castrated by surgery either earlier or later had lower body weight and weight gain than control group. However, the entire lambs had higher dressing and neck percentage than castrated ones. Early castrated lambs had significantly (P<0.0) heavier breast percentage than late castrates or entire lambs. While, late castrated ones had significantly (P<0.01) heavier loin and leg percentages than entire or early castrates. Surgical castration decreased bone weight in loin than Burdizzo castrated or entire lambs. The dimensions of the carcass was not affected by the either castration method or age, except, the L. dorsti area and back length which decreased by early castration than in later castration or entire lambs. The entire lambs had higher L, a and b values than other groups, moreover, early castrated lambs had lower L-value and electrical conductivity after 24 h than late castrated or entire lambs. The fat deposition in intestine, amentum and around kidney was significantly higher in Burdizzo early castrated lambs than all groups. On the other hand, the free fatty acids were significantly higher (P<0.05) in the prenephric and subcutaneous fats in entire than early or castrated lambs.

12/7 VARIETAL EVALUATION OF FLAX UNDER DIFFERENT ENVIRONMENTAL CONDITIONS

L.M. Zaky

Biological Sciences and Geology Department, Faculty of Education, Ain Shams University

Four flex varieties ( giza 5, giza 6, giza 7 and giza 8) were grown in giza governorate (giza site) and kafr el-sheikh governorate (sakha site) during the two successive seasons 1991/1992 and 1992/1993 to study the effect of environmental conditions on yield quantity and quality. Results obtained are summarized as follows: 1) the four flax varieties were significantly different in total plant height, technical length, straw yield/fad, and fiber yield/fad, giza 7 ranked first in the above-mentioned characters at the two locations followed by giza 8, giza 5 and giza 6 cv. 2) there were significant differences between the four varieties in the number of capsules/plant, 1000-seed weight, seed and oil yields/fad, at the two locations, giza 8 recorded the highest mean values in the four traits. 3) varietal significant differences were found in all technological characters, maximum estimates for fiber length and fiber fineness were obtained by giza 7 while giza 8 was ranked first in fiber strength, oil percentage and iodine value, either 5 giza or giza 6 exchanged their positions between the third or the fourth in this case. 4) the mean values means that great differences had occurred in the average from one variety to another.

13/7 PURIFICATION AND GENERAL PROPERTIES OF DEXTRANASE FROM ASPERGILLUS FLA VIPES

A.A. Khalil and S.H. Saad El-Deen

Biological Science Department, Faculty of Education, Ain Shams University, Roxy,

Cairo, Egypt.

An extracellular   dextranase from Aspergttlus flavipes was obtained when grown on dextran containing medium. The enzyme was purified 26-fold with a content of 42% of the original. Maximum enzyme activity was achieved at pH 5.5 and 60°C. The enzyme has a good stability at pH ranged between 4 and 6.5. A high degrees of stability were recorded at temperatures less than 60°C. Km value of the purified .enzyme was calculated to be 0.29 mM. Enzym activity was increased in presence of Ca+2 and Co*2,   whereas Hg+2 caused an inhibitory effect sulphydryl group in active sites of the target enzyme was indicated by recorded inhibition of iodoacetate and Eg2. Sharing of carbohydrate moiety of the enzyme protein has been confirmed indicating that it is a conjugated protein.

14/7 RE-USE OF WASTEWATER OF FOOD INDUSTRIES FOR THE PRODUCTION OF FUNGAL BIOMASS AND ENZYMES FOR THE BIO-TREATMENT OF WASTEWATER

M.S. Azab

Botany and Microbiology Department, Faculty of Sciences, Al-Azhar University, Cairo, Egypt

Wastewater of potato-factory (Chips) and similar food industries, separately or in combination have been re-used as a liquid medium for growing-up four fungal strains. In chips-wastewater, Aspergillus niger. A. terreus, Rhizopus sexualis and Alternaria humicala showed higher growth and enzymatic activities as compared to Czapek^s Dox liquid medium. Furthermore, good yields of fungal biomass and enzymes (a-amylase, protease and lipase) were obtained from A. terreus or R. sexualis in different types of wastewater. One % of concentrated culture filtrate as enzyme pool was added to raw potato-chips wastewater, in order to treat or improve its characters. The important parameters of the wastewater e.g. COD and BOD were improved by 24.13% to 66.92% depending on the organism and the culture conditions. The obtained fungal biomass as biosorbent has been used to remove (biosorb) up to 98.3% chromium, 92.7% iron. 96.2% nickel and 97.5% zinc ions from the industrial wastewater of galvanizing company in El-Sadat City. In this particular, food- industrial wastewater was considered as a cheap source for valuable fungal products from one hand, and as a new trend in the treatment and reuse of waste-water of many industries to solve one of the environmental problems on the other hand.

15/7 OUTER MEMBRANE ALTERATIONS IN

MULTIRESISTANT MUTANTS OF PROVIDENCIA STUARTII

SELECTED BY NORFLOXACIN

M.A.M. Yassien and H.H. Radwan*

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University and*Department of Microbiology, Faculty of Pharmacy, Helwan

University, Cairo, Egypt.

Spontaneous multiple-drug-resistant mutants were isolated from two clinical Providencia stuartii (PS132 and PS2QO) isolates on agar plates containing norfloxacin. The norfloxacin resistance achieved in the selected mutants from both PS 132 and PS200 parent strains was associated with cross-resistance to other quinolones (ciprofloxacin and ofloxacin) and ft-lactam antibiotics (cefoperazone, cephamandole, ceftazidime, and cefotaxime). The outer membrane proteins analysis showed an overproduction of 45 and 47 KDa outer membrane proteins in the selected PS132 mutants as compared with their parent strain. While in the PS200 mutants, only the 45KDa outer membrane protein was overproduced. Also, the selected mutants had about 3- to 4-fold lower rate of norfloxacin uptake than that of the parent strains. The norfloxacin uptake by the mutli-resistant mutants increased after addition of 0.25 mM Carbonyl cyanide m-chlorophenylhydrazone. These data suggest that overproduction of 45 and 47 KDa outer membrane proteins in P. stuartii strains is associated with cross-resistance to fluoroquinolones (norfloxacin, ciprofloxacin, and ofloxacin) and /3-lactam antibiotics (cefoperazone, cephamandole, ceftazidime, and cefotaxime).

16/7 A NEW RECORD OF A BACTERIAL ALKALINE THERMOSTABLE XYLANASE FROM AN EGYPTIAN SOIL.

M.S.A. Shabeb

Botany Department, Faculty of Science, Aswan, South Valley University.

Twenty seven isolates were isolated as an alkaline xylanase-producing microorganisms from soil samples and hemicellulosic materials, identification was carried out by comparison with reference strains cited in bergey's manual of systematic bacteriology (1986). The present isolates were investigated for their xylanase activity in three stages using wheat bran extract agar medium with xylan. one of the isolates, identified as bacillus caldotenax bs - 48 produced 118 times more xylanase activity than other isolates, maximum enzyme produced was observed after 96 hours of growth withph range of 9 -12 at 55 °c. the crude enzyme was stable for 30 minutes at 55°c andofph of 8 -11 for more than 2 hours, thus, it is believed that the present xylanases may be considered as a new record alkaline thermostable xylanase from an egyptian soil.

17/7 FLUCTUATIONS IN ENDOGENOUS HORMONES OF PEA SEEDS AND SEEDLINGS FOLLOWING PRE-SOWING COLD TREATMENT UNDER DIFFERENT MOISTURE CONTENTS

A.A. Mohsen and L.M. Zaki*

Faculty of Science, Tanta University

* Faculty of Education, Ain Shams University

Results of this experiment revealed that the fresh -weight of pea seeds, stored in the dark at 5°C under different moisture levels increased with increasing moisture content while the dry weight decreased Storage of pea seeds at 30% moisture had no effect on the percentage germination, whereas 50% moisture significantly lowered the percentage germination but 10% moisture was slightly stimulatory. Radicle length of 7-day old seedlings increased at 10% moisture and decreased by raising the moisture level to 30% or 50%. However, the fresh weight was insignificantly affected by the cold treatment at 50% whereas 10% or 30% moisture increased the fresh weight gain compared to dry seed storage. Meanwhile, the dry weight of the 7-day old seedlings decreased gradually with increasing moisture level. In addition, the content ofDNA and RNA of primary leaves increased under 10% and 30% moisture content but DNA content decreased and RNA content increased under 50% moisture. Studies of endogenous hormones in pea seeds and 7 day old seedlings indicated that the levels of the phytohormones (auxins, gibberellins and cytokinins) markedly increased at 30% moisture and decreased at 50% moisture coupled with increased level of growth inhibitors under the latter condition. Such changes in growth criteria and pattern of endogenous hormones revealed that the moisture level around the seeds during the period of cold storage interferes with the germination of seeds and the growth of seedlings.

18/7 CYTOLOGICAL AND BIOCHEMICAL EFFECTS OF 5-FLUOROURACIL AND COLCHICINE ON V1CIA FABA PLANTS

M.M. Shehata, A.A. Habib, N.S. Khalifa and M.S. Salama*

Botany Department and *Entomology Department, Faculty of Science, Ain Shams

University, Abbassia, Cairo, Egypt.

The present investigation has been designed to evaluate the cytological and biochemical effects of a well known antimitotic agent (colchicine) and an antitumour agent (5-fluorouracil) on Viciafaba cells. Four concentrations of 5FU (5, 2.5,1.25 and 0.625mg%) were used either alone or in combination with 0.05% colchicine to demonstrate their effects on mitosis in root tip cells, M2 seed storage protein content and protein banding patterns. The data obtained indicated that the medicinal drug 5FU either alone or in combination with colchicine had pronounced effects on mitotic indices (MI), frequency and types of mitotic phases, types and frequency of mitotic abnormalities (stickiness, bridges, c-metaphase, breaks, star metaphase and micronuclei). Changes in the total protein contents and protein profiles of M2 seeds \vere also observed. The electrophoretic separation of bands reveal both quantitative and qualitative changes. The latter was expressed by the disappearance or appearance of certain bands, changes in band intensities and mobility rates and molecular weights. 5FU also can counteract the effect ofcolchicine.

19/7 GROWTH CHARACTERISTICS, ENDOGENOUS PHYTOHORMONES, MITOTIC DIVISION AND CHROMOSOMAL ABERRATIONS OF RAPHANUS SATIVUS L. PLANT EXPOSED TO HEAVY METAL STRESS.

A.A. Hassanein and M.M. Shehata

Botany Department, Faculty of Science, Ain Shams University, Egypt

This work was carried out to study the effect ofCa (0.5, 1.0 and 2.0 mM) and Pb2* (5, 10 and 20 mM) on growth parameters, endogenous phytohormones and the activities of the related oxidative enzymes on white radish (Raphanus sativus L.) plants. In addition, the effects of the previously mentioned concentrations of both heavy metals on mitotic cell division and chromosomal aberrations were also studied. Growth parameters were significantly inhibited by all concentrations of heavy metal applied Cd2+ and Pb2+. Moreover, IAA, GAj, zeatin andzeatin riboside contents were reduced sharply, while additional amounts of abscisic acid were detected concomitantly with enhanced IAA oxidase andperoxidase activities in both roots and tops of white radish plants compared with those of the untreated control. These changes were increased as the concentrations of both heavy metals increased. Mitotic mdices were significantly decreased in roots treated with all concentrations of heavy metals applied for 4 and 24 hours. Both heavy metals Cd2+ or Pb2^ showed clear effect on the percentage of different mitotic stages and induced a wide range of mitotic abnormalities as metaphase stickiness diploid c-metaphase, multipolar anaphase, laggards, micronuclei, destroyed nuclei and ana-telophase bridges. Finally, Ca(NO^2 can alleviate to some extent the inhibitory effects of Co2* and Pb2* on white radish plant growth, in particular cell division, phytohormones, oxidative enzyme activities and chromosomal aberration.

20/7 THE CORRELATIVE CHANGES ASSOCIATED WITH BUD DORMANCY AND ROOTING OF CANE CUTTINGS IN GRAPEVINE

H.I. Khattab, M.M. Emam and M.M. Shehata

Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt.

Grapevine (Vitis vinifera L.) has a fairly predictable cycle of growth. The dormant season begins in autumn in temperate regions. Segments of the main stem, lateral buds and leaves of grapevine were collected monthly during the growth cycle (from October to February). Explantfrom segments of the main stem also were tested for rooting capacity. Studies demonstrated that during the period of rest, the cane water content decreased whereas, abscisic acid (ABA) increased sharply as the intensity of bud dormancy deepened. During the endodormancy period, the inhibitor -promoter balance of hormones is weighed in favor of the inhibitor, but at the end of endodormancy, the balance is shifted in favor of the promoter. Changes in carbohydrates, total soluble protein, genomic DNA and mineral contents were also investigated. Starch concentration decreased as the intensity of bud dormancy increased, while the concentration of soluble sugars (glucose and sucrose) increased. The charaterization of different protein fractions were obtained using SDS-polyacrylamide gel electrophoresis. Fifteen polypeptide bands of molecular weight ranged between 47.36 and 2. 01 kDa were observed in the dormant buds and canes of Vitis venifera L. Also, the present work, studied the potential of the restriction endonuclease analysis using four restriction enzymes (Bam HI, Hind III, Xma I and Sma I). The highest degree of cleavage was observed in buds which may indicate the presence of stimulators in dormant buds facilitate the recognition of the nucleotide sequence. Calcium, potassium, magnesium, sodium, phosphorus and boron decreased in the dormant buds, cane and old leaves of grapevine. The canes can form adventitious roots in December, January and February naturally, however, the canes treated with either 10 yM NAA or 10 pMIBA succeeded in producing adventitious roots in all the test months. Application of a combination of 5 fiMIBA andS/iMNAA was the most suitable treatment for grapevine rooting.

21/7 CHROMOSOMAL CRITERIA IN RELATION TO SEED PROTEIN OF SOME EGYPTIAN CULTIVARS OF V1C1A FABA.

A.I. El-Nahas

Biological Sciences and Geology Department, Faculty of Education, Ain Shams

University, Roxy, Cairo, Egypt.

In the present invesTigatlon 12 egyptian Viciafaba cultivars viz: Giza blanca, giza 2, giza 3, giza 40, Giza 461, giza 643, Giza 667, giza 674, Giza 714, giza 716, Giza 717 and giza 843 were studied. The study based on chromosome analysis and protein profiles using sds-page technique. In all cultivars 2n = 12 or 14 was observed, in addition to the presence of one b chromosome In some cultivars, one to three satellited chromosomes were recorded in some as well. Root length showed variable values being the highest In three cultivars that have a b chromosome each. Protein profiles revealed differences among cultivars. Unique bands are characterization of the different cultivars.

22/7 BIOACCUMULATION OF SOME HEAVY METALS AND

THEIR INFLUENCE ON THE METABOLISM OF SCENEDESMUS BIJUGA AND ANABAENA SPIROIDES

A.A. Fathi, F.T. Zaki* and A. Fathy**

Botany Department, Faculty of Science, El-Minia University, Egypt.

*Botany Department, Faculty of Science, Cairo university, Egypt.

**Botany Department, Faculty of Science, Alexandria University, Egypt.

The results of this investigation clearly show that the higher doses (10~7 and 10~

of cobalt, mercury and vanadium strongly affected dry weight, pigment, protein, amino acid and sugar accumulation in Anabaena spiroides and Scenedsmus bijuga. Cobalt and mercury accumulation by Anabaena was much higher than that of Scenedesmus, while vanadium accumulation showed no much difference between the two species. The inhibitory effect on phosphatases activity was more prominent for both organisms with mercury than the other two tested metals.

23/7 INHIBITORY EFFECTS OF TANNIC AND GALLIC ACIDS AND RELATED COMPOUNDS ON BIOLOGICAL ACTIVITIES

OF THE CVANOBACTEKWM ANABAENA ORYZAE

F.T. Zaki and A.A. Fathy*

Botany Department, Faculty of Science, Cairo University.

*Botany Department, Faculty of Science, El-Minia University.

Treatments of Anabaena oryzae with subinhibitory concentrations oftannic and gallic acids, methyl gallate and propylgallate resulted in a severe decline in biological production, attributable to the inhibition of biosynthesis and biomaterial leakage. Treatments of Anabaena oryzae with % ICS of gallic and tannic acids resulted in a remarkable inhibition of total cell yield and protein content by about 68% and 63% respectively. Gallic acid showed more inhibitory effect on chlorophyll "a" and carotenoids than other tannin compounds. Transaminases and nitrate reductase activities of the Cyanobacterium were inhibited by all tannin compounds in a concentration dependent manner. The control ofbiomass accumulation in relation to the production of off-flavor compounds in cyanobacteria by natural tannin compounds may have important aquacultural implications.

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