Vol. 41, June, 2012.

viagra suisse romande 1/41 PROTEASE PRODUCTIVITY BY SOME THERMOALKALOTOLERANT GRAM POSITIVE BACTERIA ISOLATED FROM MANURE

Mamdouh S. El-Gamal, Esam H. Abdel-Shakour, Mohamed A. Abdel-Rahman and Ahmed A. Attia

*Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt

The purpose of the present study was to investigate protease production by some bacteria isolated from manure samples obtained from different localities (El-Beheraa governorate). Eighty seven (87) isolates were obtained. Screening for the most potent isolates was based on their abilities to grow at high temperature up to 70oC. The most potent isolates sildenafil prix suisse St 1-3 & C 3-2 were identified to be related to the genera meccanismo d'azione del levitra Brevibacillus borstelensis & Bacillus licheniformis was based on 16S rRNA sequence analysis. The maximum protease production by ou acheter cialis a paris Brevibacillus borstelensis St 1-3 & Bacillus licheniformis C 3-2 were obtained at temp. 55oC, pH 8 and 8.5 respectively, 0.5% glucose as carbon source,   0.5% urea as nitrogen source, 100 ppm of Ca+2 & Mg+2 as metal cofactor, 1% gelatin as substrate, 4% inoculum size, 24 h. incubation period, 500 ml flask capacity , under shaking condition . Ammonium sulphate precipitation at (80%) increased specific activity by 1.83 and 1.86 folds, respectively, while, further purification by gel filtration using Sephadex-G200 increased the specific activity by 9.05 & 10.46 folds for both fausse ordonnance pour du cialis Brevibacillus borstelensis St 1-3 & Bacillus licheniformis C 3-2, respectively. Analysis of purified proteases revealed an apparent molecular weight of 47 KDa for both achat cialis 20mg pas cher Brevibacillus borstelensis St 1-3 & Bacillus licheniformis C 3-2, finally,amino acid type and their concenrteation were detrmined for each protease enzyme .

nom des medicaments viagra 2/41 COMPARATIVE IN VITRO STUDY ON THE ADHESION OF SOME PROBIOTIC AND GRAM-NEGATIVE BACTERIA

Hoda Mahrous

Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, Menuofiya University, Egypt,

Adhesion to the human intestinal cell is considered as one of the main selection criteria of lactic acid bacteria for probiotic use. The ability of six strains of prix du cialis maroc Lactobacillus spp. two strains of effets du cialis 5 mg Pseudomonas spp. and three strains of acheter du viagra en inde E.coli to adhere to human intestinal mucous was examined. The highest adhesion demonstrated for Lactobacillus pentosus P191 compared to the reference probiotic strain Bifidobacterium bifidum (B) while, Lb. acidophilus P106 and Lb. plantarum P164 recorded high adhesion values, followed by Lb. salivarius P116 then Lb. brevis P102 ended with Lb. pentosus P 160. The adherence of E.coli strains and Pseudomonas spp. strains were low with comparative to most of probiotic tested strains.

3/41 MICROBIOLOGICAL SAFETY AND QUALITY OF WATER SUPPLY AND WATER COOLERS IN DIFFERENT SCHOOLS OF ALMAJMAAH GOVERNORATE IN SAUDI ARABIA

El-Safey Mohamed El-Safey, Khalid Mohamed AlJaralah*, Abdullah Ahmed Aldahash** and Mohamed Othman AlRukban***

Faculty of Science, Al-Azhar University, Assiut 71511, Egypt. (Now, on secondment to College of Applied Medical Science, Majmaah University, AlMajmaah, 11952 (66), KSA)

*College of Applied Medical Science, **Community College and ***Medicine College, Majmaah University, AlMajmaah, 11952 (66), KSA

Providing safe drinking water to the public is one of the major challenges of the 21st century. Thus, the impact of contamination on public health receive an increased attention of worldwide. Bacteria and fungi are responsible for most of the contamination incidences associated with drinking water. The main purpose of this study is to assess the microbiological quality of water supply and drinking cooling system water in different schools of Almajmaah governorate in Saudi Arabia. One hundred samples of drinking water supply and cooling systems samples were collected and analyzed. The identification of bacterial and fungal isolates were based on morphological and biochemical methods. In total, 55 fungal isolates belonging to 7 genera were detected in a total 100 water supply and drinking water cooling system samples, including species of Penicillium, Aspergillus, Fusarium, Phialophora, Trichoderma, Acremonium and Cladosporium. On the other hand, Thirty-Two bacterial isolates represent eight genera, this including species of Staphylococcus, Bacillus, Pseudomonas, Escherichia coli, Salmonella, Serratia, Yersinia and Enterobacter. All bacterial isolates were subjected for antimicrobial susceptibility and growth inhibitors, five isolates are resistant to cefazolin, three isolates is resistance to Penicillin G, four isolates are resistance to cefonicid and three isolates are resistance to ceftazidime. The most effective microbial growth inhibitors are sodium azide, ZnCl2, CdCl2, NiCl2, CuC12 and FeCl2 (at 500, 200, 100 and 50 μ/ml). On the other hand, EDTA and urea, failed to exhibited any effect on the growth of all the bacterial isolates. The results of this study suggest that consumption of water supply and its cooling system could represent a notable risk to the health of consumers. Therefore, the microbiological safety and quality of water supply and cooling system should be considered.

4/41 PRODUCTION, PURIFICATION AND CHARACTERIZATION OF ISOAMYLASE ENZYME BY THE ALKALITOLERANT MICROCOCCUS VARIANS

Magdy, A. Gadallah, Eman, A. Ghazy, Reda, A. Bayoumi*, Samier, S. El-Louboudy* and Mostafa M. Abo Elsoud

Microbial Biotechnology Dept., NRC, Egypt.

*Botany and Microbiology Dept., Faculty of Science, El-Azhar Univ., Egypt.

Alkaline isoamylase [amylopectin-6-glucanohydrolase, EC 3.2.1.9] activity was detected in the culture medium of an alkalitolerant strain of Micrococcus varians, which was isolated from Egyptian soil sample. The enzyme was purified to homogeneity from the culture filtrate by ammonium sulfate precipitation, DEAE- cellulose and sephadex G-100 and characterized. The purified isoamylase had a pH optimum y 8.6 and maximum activity at 50ᵒC. The purified isoamylase had a molecular mass of 97 KDa as determined by SDS-PAGE. The purified isoamylase had Km (50ᵒC, pH 8.6) for amylopectin 3% (W/V) was 6.013 and Vmax 11.5.Na, Ni, Fe, Mg and lead inhibited the enzyme activity of isoamylase, while Ca, K, and Zn activated it.

5/41 MODULATION OF RADIATION INJURY IN PREGNANT RATS AND THEIR FETUSES BY BONE MARROW TRANSPLANTATION

Nehal A. Abuo El Naga and Mervat A. Abd Rabou

Zoology Department, Faculty of Science, Al- Azhar University

This work was conducted to evaluate the possible protective role of bone marrow transplantation (BMT) against whole body γ-irradiation (2Gy) in pregnant albino rats and their fetuses at two different gestation periods. Different treatments were performed on day 7or 14 of gestation and examined at the end of the gestation period (day 20). A significant decrease was recorded in the length of embryos, their tails and weight of maternal liver with a non significant decrease in the number of implantation sites and live fetuses on day 7 or 14 of gestation. Pregnant rats exposed to 2Gy γ-rays and treated with BM one hour post-irradiation showed non-significant changes in implantation sites and number of live fetuses on day 7 or 14 of gestation. Improvement in the length of fetuses, their tails and weight of maternal liver on day 7 of gestation were observed with less improvement on day 14 of gestation compared to the control group. Also, radiation caused many histological and histochemical changes in the liver tissues of mothers and their fetuses on day 7 or 14 of gestation. Ameliorated histological and histochemical results were obtained by using BMT post –irradiation.

6/41 BIOCHEMICAL AND MOLECULAR DIFFERENCES BETWEEN EGYPTIAN CLOVER HYBRIDS

Zeinab M.Abd El-Naby, Ehab M. Zayed and Salah S.M. Abo-Feteih

Forage Crops Research Department, Field Crops Research Institute, Agriculture Research Center, Giza- 12619, Egypt.

The goal of this study was to examine the differences between the Egyptian clover hybrids at the molecular level. Two Egyptian clover genotypes, multi-cut variety Sakha-4 belonging to type Miskawi and single cut berseem variety Fahl were reciprocally crossed. F1, F2, Bc and reciprocals along with the two parents were studied and analyzed. Enzymes analysis was performed on 10% native-polyacrylamide gel electrophoresis (Native-PAGE), to detect variation in Peroxidase, α- and β- Esterase. ISSR-PCR reactions were conducted using five primers. Amplification was performed in 25µl reaction volume. Electrophoresis profile of peroxidase, α-Esterase and β- Esterase revealed 2, 9 and 9 bands, respectively. Analyses of ISSRgave a total number of 60 bands from five primers. The number of polymorphic bands was 44; while polymorphism percentage was 73.4%. Combined analysis of dataillustrated that the highest percentage of similarity between the genotypes was F2MM Mother Miskawi and F1 Mother Miskawi (0.927).The lowest value of the similarity over genotypes was between PM and PF (0.615). The positive and negative values of the Skewness and Kurtosis results indicated a "flat" distribution for all genotypes, except BC1MF (Backcross Mother Fahl) and F1MM (First generation mother Miskawi) and the data are skewed left.

7/41 MOLECULAR ANALYSIS OF GENETIC VARIATION IN POTATO CULTIVARS INFECTED WITH VIROID IN EGYPT

Samier H. Abdel-Aziz

Botany Department, Faculty of Science, Benha University, Egypt.

Four cultivars of the cultivated potato grown in Egypt were screened of genetic analysis using peroxidase isozymes and ISSR-PCR. The use isozyme separation to detect genetic variation among potato cultivars and to distinguish each cultivar of Potato spindle tuber viroid (PSTVd) infection were evaluated. DISC-PAGE isozyme separation of Cara, Nicola, Diamond and Spunta cultivars, showed 5,7,8 and 9 peroxidase isozymes in PSTVd inoculated plants compared with 7, 6, 5 and 6 peroxidase isozyme in healthy ones respectively. Similarity analysis confirmed the difference in isozyme patterns among potato cultivars. Three ISSR primers Only (among 10 tested) were chosen as producing polymorphic DNA-fragments differentiating the investigated cultivars. Based on these identity markers, the genetic relationships between cultivars. The phylogenetic tree revealed that the four studied cultivars showed close similarity with the group. Although minor viroid susceptibility variations were recorded in the cultivars of some clones. The developed ISSR profiles indicated that Spunta was lowest sensitive for PSTVd infection followed by Diamond and Nicola compared with Cara potato cultivars. Potato cultivars were genetic analysis for affect with PSTVd. Potato cultivars Cara, Nicola, DiamonD and Spunta were mechanically inoculated with PSTVd, revealed that the external symptoms PSTVd infected potato were leaf narrow leaf, rugosity and dark green, stem stunting and erect growth as well as, spindle and deformation of potato tubers. These results were confirmed by dot blot hybridization using DiG Labeled cDNA (specific PSTVd probe). Symptoms severity were assessed by measuring various foliage and tubers parameters. Disease severity caused by PSTVd ranged from mild severe depended on potato cultivars. Cara potato cultivar was susceptible, Nicola was moderate, Diamond and Spunta were tolerant to PSTVd infections.

8/41 ULTRASTRUCTURAL CHANGES IN TOMATO (LYCOPERSICON ESCULENTUM L.) CALLI DURING ORGANOGENESIS

Islam I. Lashin

Botany and Microbiology Department, Faculty of Science Al-Azhar University,

Nasr City, Cairo, Egypt

In vitro culture response was assessed intomato (Lycopersicon esculentum L.) for optimum callus induction and plantlet regeneration. Callus cultures were established from different explants of tomato. Explants i.e. leaves, petioles and roots, were cultured on MS medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA) singly and in combination. The highest callusing percentage was obtained with petioles explants cultured on MS medium supplemented with 2,4-D at 1 mg/l and BA at 1 mg/l. After 4 weeks of culturing, organogenesis was observed only on the explants cultured on medium containing different concentrations of BA alone. The best shoot formation (78%) was obtained for petiole explant callus induced on MS medium containing BA at 2 mg/l. The cell ultrastructure incallus obtained frompetioleexplants has been studied. The ultrastructure of embryogenic callus showed tissue contained cells with a large vacuole, spherical nucleus. The chloroplast contains starch grains and cell wall of uniform thickness and numerous plasmodesmata.
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