Vol. 42, October, 2012.

le bienfait du viagra 1/42 LOCALIZED CORROSIONOF MILD STEEL INDUCED BY P. MARGINALIS EXOPOLYSACCHARIDE PRODUCER

Eman A. Ghazy, Nabil A. El Manakly*, Manal G. Mahmoud, Osama H. Elsayed and Mohiy E. AbdelSamaei

Microbial Biotechnology Dept. National Research Center, Cairo, Egypt

*Electrochemistry and Corrosion Dept. National Research Center, Cairo, Egypt

prix du cialis en pharmacie à lyon Pseudomonas marginalis was a bacterium which produces exopolysaccharide (EPS). Accordingly; it is studied for the production, isolation and chemical characterization of its EPS. delai d'action du levitra Pseudomonas marginalis is identified on basics of its morphology and biochemical characteristics. It has the ability to produce an extracellular biofilm when it is grown on a basal broth medium for 7 days at 30oC.A crude biofilm is recovered from the culture supernatant by ethanol precipitation with a yield of 1.7 gl-1. The structure of EPS is identified by sugar composition analysis, HPLC and IR analysis. The results of HPLC and IR showed that the contents of mannouronic acid and rhamenose are in an approximate molar ratio of 1:3. Accordingly. It was suggested that the biofilm from acheter du cialis online Pseudomonas marginals could be useful in environmental application, especially in the bioremediation of oil-polluted seawater, their great application potential in food, cosmetic, pharmaceutical and oil industries, where they are used as thickening, stabilizing and emulsifying agents, (Yu et al., 2007). On the other hand obtained results suggested that EPS biofilm accelerates corrosion behavior rate mild steel under saline sea water conditions.

commandé du viagra 2/42 MODIFICATIONS IN MAIZE PROTEIN PROFILES AND ISOZYMES AS RESPONSE FOR SALT STRESS

Samir H Abdel-Azizand Omar S. Ebshish

Botany Department, Faculty of Science, Benha University, Egypt.

Some plants posses' salt relative tolerance depends on their contents of solid substances. Proteins play an important role in this matter. The study of protein profiles electrophoretically may leads to pick a picture for what is happen inside tolerant and sensitive plants as response for salt stress. In this investigation salt tolerances for some maize cultivars planed to be studied by subjecting these plants in salt stress and compare their performance under salt stress with the control ones. The two most contrasting cultivars in salt tolerance will be selected according to their performance in some yield related traits. The selected cultivars will be subjected to biochemical analysis using water soluble and non water soluble proteins electrophoretically and the zymogram of some isozymes to find out some biochemical markers related to salt tolerance in maize. This work was aimed to study the effects of salt stress on plant growth. The changes in corn genes expression as response for salt stress conditions as compared to non salt stressed plants by investigate the total soluble and non soluble protein banding patterns using Sodium Deudocyle Sulfate poly acrylamide gel electrophoresis (SDS-PAGE). Also the changes of zymogram patterns of some isozymes under salt stress as compared to non salt stressed plants.
prendre la moitié d'un viagra

meilleur site pour cialis en ligne 3/42 ISOLATION AND CHARACTERIZATION OF ABIOTIC STRESSES RESPONSIVE GENES FROM ACACIA NILOTICA

Ahmed M. Hashem, Naglaa A. Ashry*, Hussein S. Hussein and Ali M. El-Refy

Biotechnology Department, Faculty of Agriculture, Al-Azhar University,

Cairo, Egypt

*Cell Research Department, Field Crops Research Institute, Agriculture Research Center, Giza, Egypt

contres indications du viagra Acacia nilotica belongs to family Leguminosae, Mimosoideae. It is capable of fixing atmospheric nitrogen, Acacia is also known to tolerate heat, salt and drought stresses. Considering the detrimental effect of drought, salt and temperature on plant growth and development, efforts have been initiated in the identification of genes that confer abiotic stress tolerance in plants remains a challenge in plant breeding. The present study focused on the identification of abiotic stress responsive genes in pharmacie medicament viagra Acacia nilotica subsp nilotica based on sequence similarity approach exploiting known abiotic stress responsive genes from model crops or other plant species. These sequences have been functionally validated and reported to play significant role in stresses response in model plants such as comment utiliser le cialis Arabidopsis and Glycine max. Five abiotic stress responsive genes identified in other plants were partially amplified from Acacia nilotica genome and their presence was confirmed after sequencing the PCR products. DNA sequence has been submitted to the NCBI database with JX539903 accession number. The similarity searching was performed to identify similar sequences vs. isolated fragment using BLASTN tool at the NCBI database. The results showed that the amplified fragment was similar to Glycine max, Medicago truncatula and Arabidopsis thaliana which are considered to be model biological systems. Translation of the fragment into hypothetical protein showed similarities with Glycosyltransferase genes, which is known to have a role in abiotic stress tolerance in plants.

 

4/42 IMPROVEMENT OF SALT TOLERANCE IN MAIZE BY ROOT INOCULATION WITH MYCORRHIZA

Samir H Abdel-Azizand Omar S Ebshish

Botany Department, Faculty of Science, Benha University, Egypt

Salt stress is one of abiotic stresses which negatively affect the growth and productivity of the commercial crops. Some plants posses' salt relative tolerance depends on their genetic potential which leads to mechanisms of tolerance. In this investigation maize cultivar (Giza 168), was grown under two NaCl concentrations (0 and 6000 ppm), in order to investigate their role in plant salt tolerance improvement.

5/42 FOLIAR APPLICATION OF SOME MICRONUTRIENTS AMELIORATES NITRATE ACCUMULATION IN RADISH AND PARSLEY PLANTS

Fawzia Ahmed Ebad, Zeinab Ahmed Khidr, *Gamal El-Din Abd Elkhalek Bador and *Nora Fathi Gaber Salem

* Botany and Microbiology Dept., Faculty of Science, Al-Azhar Univ.Cairo, Egypt.

**Soil, Water and Environment Research Institute, Agric. Research Center, El-Mansoura, Egypt.

Nitrate accumulation in Egyptian vegetables shows considerable high values, compared to those found in vegetables grown in several foreign countries. This could be mainly due to the intensive application of nitrogen fertilizers to plants. Therefore, a pot experiment was carried out in the greenhouse of Soil, Water and Environment Institute at El-Mansoura to study the effect of foliar application of Mo, Fe & Mn on the accumulation of nitrate and nitrite in radish and parsley plants. This aimed to adjust nitrate level to the permissible limits, since these micronutrients have essential roles on the formation and activity of nitrate reductases. Foliar application of Fe, Mo and Mn either solely or as a mixture led to significant decreases in the concentrations of nitrate and nitrite in the two plants under study, compared to the control. Foliar application of the mixture of the micronutrients studied was superior for increasing the activity of nitrate reductases in radish and parsley plants, followed by sole application of Mo, Fe, Mn, respectively. It could be concluded that application of such micronutrients in the leafy vegetable crops significantly decreased the accumulation of nitrate in plants. This is mainly due to the role of such micronutrients for activating the nitrate reductase effect in decreasing the nitrate and nitrite accumulation.

 

6/42 ISOLATION AND CHARACTERIZATION OF STREPTOMYCES SPECIES ANTAGONISTIC PATHOGENIC STAPHYLOCOCCUS SPECIES

Gamal M. El-Sherbiny

Department of Botany and Microbiology, Faculty of Science (boys), Al-Azhar University, Madient Nasr, Cairo, Egypt.

In this study, fifteen Streptomyces isolates were obtained and purified from soil sample from North Western Mediterranean coast in Egypt 2010. Among these isolates, the isolate number 11 was antagonistic against pathogenic microorganisms including Gram positive and negative bacteria and unicellular fungi, while it was moderately active against filamentous fungi. This Streptomyces isolate was identified as Streptomyces avermitilisand characterized using different International Streptomyces Project (ISP) media, as well as theother morphological, physiological & biochemical characteristics of the isolatethat could be detected. From the taxonomic features of the isolate it was found thatits characters match with Streptomyces avermitilis so it was given thegeneric name Streptomyces avermitilis AZGM-11. It was found that the bioactive compound was produced by soil isolate Streptomyces avermitilis AZGM11. Once this active compound was recovered by ethyl acetate from the fermented broth, it was purified by paper, column and thin layer chromatography. Structure was characterized by analyzing UV, IR, MS spectra and elemental analysis. During analysis, such active compound showed strong activity against Gram-positive and Gram-negative bacteria, Staphylococcus species causing pyogenic infection, as well as unicellular but low active against filamentous fungi.

 

7/42 HYPERHYDRICITY PHENOMENON PROBLEM IN EMBRYONIC CALLUS OF DATE PALM, SOLVING BY GLUTAMINE AND NH­­4+ : NO3- RATIO IN MS BASAL NUTREINT MEDIUM

Zeinab E. Zayed, Maiada M. El-Dawayaty and Lobna M. Abdel-Gelil

The Central Lab of Date Palm Researches and development, Agriculture Research Center, Giza, Egypt.

Hyperhydricity is a serious problem faced in vitro date palm propagation which directly effects on the commercial production. So we try to solve this problem by studying, effect of glutamine as the organic source of nitrogen and NH­­4+ : NO3- ratio as the inorganic source of nitrogen to decrease hyperhydricity phenomenon and to produce normal somatic embryos of date palm cv. Gundila. Vitrified embryonic callus were inoculated on MS basal nutrient medium modified with glutamine levels and NH­­4+ : NO3- ratio. Five concentration ratios of NH4 : NO3 (825:1425, 1237.5:950, 0:1900, 1650:0, 0:0 ml/l) were used with 0.1 mg/l NAA for 8 weeks throughout 2 recultures. There were gradually increasing in the percentage of vitrified embryonic callus differentiation to normal somatic embryos by increasing glutamine levels from 0.0 to 400 mg/l. Glutamine at the lowest level (50mg/l) significantly increased the number of vitrified somatic embryos. Ammonium as the sole source of N resulted in depression in somatic embryos differentiation and escalated the frequency of hyperhydricity whereas, if nitrate was used as the sole N source, somatic embryos good quality were produced and hyperhydricity was eliminated

 

 

8/42 EXTRACELLULAR LIPASE FROM BACILLUS SUBTILIS: PRODUCTION, PURIFICATION AND CHARACTERIZATION

EI-Safey M. EI-Safey

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt.

       Production and partial purification of Lipase enzyme by Bacillus subtilis was the aim of this study. Bacillus subtilis was allowed to grow in broth culture for purpose of producing Lipase enzyme. Optimal conditions for Lipase production by Bacillus subtilis were an optimum substrate concentrations 0.1 % (8.12 units/ml-1-1); optimum incubation period, 18 h (266.07 units/ml-1). An optimum incubation temperature was 40°C (426.57 units/ml-1); the best buffer for production of Lipase enzyme was phosphate buffer at pH 7.0 (5308.84 units/ml-1). An optimum inoculum size was 0.5 ml-1 from stock suspension of Bacillus sulbtilis (7 x103/ ml-1) (31.62 units/ml-1); an optimum inoculum age 24 h (31.62 units/ml-1). 500 ml-1 was the optimum fermentor (flask) capacity (aeration) (26.60 units/ml-1); the best-medium volume 100 ml-1 (79.43 units/ml-1). The best broth ingredient was beef extract and NaCl (926.82 units/ml-1); An optimum carbon sources was casine (21.87 units/ml-1); an optimum nitrogen source for Lipase production was (NH4)2 SO4 (21.87 units/ml-1); Glutamic acid (426.57 units/ml-1) was the best amino acids for production of Lipase enzyme. On the other hand, the organic acids decreased Lipase production at different concentrations as enzyme inhibitors. There is no productivity, was observed with lactic, citric and acetic acid at 0.5 %, 1.0% and 1.5%, respectively. The Lipase enzyme was purified by ammonium sulfate precipitation and sephadex G100 and G200 filtration. A trial for the purification of Lipase resulted in an enzyme with specific activity of 6381.75 (units/mg prot/ml-1) with purification folds 7.87 times. The Lipase activity increased as the increase in enzyme concentration; optimum substrate concentration was 0.l% (w/v) (426.57 units/ml-1); an optimum incubation temperature was 40°C (1318.25 units/ml-1); purified Lipase enzyme had a maximum activity at pH 6.6 of phosphate buffer (5308.84 units/ml-1), and the optimum incubation time was 36 h (66.83 units/ml-1). Data emphasized the possibility of the production and purified microbial Lipases enzymes for application under industrial scale.

9/42 PURIFICATION AND BIOCHEMICAL PROPERTIES OF T. REESEI Α- AMYLASE

Afaf S. Fahmy, Zeinab A. El-awamry*, Ebtsam N. Hossiny*, Nevin M. Farid,

Somia S. Abdel-Ghany, Roqaya, I. Bassuiny, Saleh A. Mohamed

Molecular Biology Dept., National Research Centre, Cairo, Egypt

*Botany and Microbiology Dept., Faculty of Science for Girls, El-Azhar University, Cairo, Egypt

Extracellular α-amylase produced by T. reesei grown on rice straw was purified through purification procedures of ammonium sulfate precipitation, DEAE-Sepharose and Sephacryl S-200 chromatography. Two forms of α-amylase designated as fractions I and fraction II were fractionated from the DEAE-Sepharose column. The molecular weights of fraction I and II of T. reesei α-amylase were estimated to be 63 and 45kDa, respectively. It was found that the enzyme catalyzed rapid hydrolysis of starch associated with a slow formation of reducing materials.Maximal activity of fraction I and II of α-amylase was obtained at pH 5.0 and 40oC. Both fractions of T. reesei α-amylase catalyzed the hydrolysis of different substrates in the order of amylopectin ≥ starch > glycogen > β-cyclodextrin > α- cyclodextrin > xylan. The two fractions showed no activity on cellobiose, dextran, dextrin or maltose. The Km value for starch, glycogen and amylopectin hydrolysis were determined and found to be 1.12 mg, 1.18 mg; 0.57 mg for fraction I and 1.30 mg, 1.19 mg ;0.68 mg for fraction II, respectively. The two fractions of T. reesei α-amylase showed complete stability after incubation for 24 h at pH 5.0. Incubation at pH values higher 7.0 showed a gradual decrease in enzyme activity. pH values of 3.5 and 4.0 caused a significant loss in activity for both fractions. Fractions I and II were fully stable when incubated at 40oC for 60 min, while at 50oC a moderate loss in activity of both fractions was observed. The two fractions were markedly inactivated when exposed to 60oC, 70oC or 80oC for 15 min. The inhibitory effect of EDTA on the activity of both fractions suggests that a metal cation is participating in enzyme catalysis. Fractions I and II were activated by Ca2+ and Ba2+. They were strongly inhibited by Mn2+, Cu2+, Al3+, Fe2+, Hg 2+ and Ni2+, but less affected by Co2+, Mg2+ and K+. Ca2+ significantly raised the thermal stability of both fractions on exposure to temperatures ranging from 60oC to 90oC. SH group seems to play a role in the catalytic action of T. reesei α- amylase as addition of iodoacetate, N- ethylmaleimide or P- chloromercuricbenzoate inhibited the activity of both fractions, while dithiothreitol increased it.



10/42 EFFECT OF VIRAZOLE ON PRODUCTION OF MICROTUBERS AND ON ISOZYME PEROXIDASE ACTIVITY IN POTATO TISSUES CV. SPUNTA

Mahmoud I. Nasr, Ibrahim A. Ibrahim, Fathy M. El-Shayeb* and

Alaa El-din Sayed Sayed Ewase Khodeir**

Genetic Engineering and Biotechnology Research Institute, Menoufiya

University, Sadat City, Egypt.

*Botany Department, Faculty of Science, Cairo University, Egypt.

**Molecular Biology Unit, Faculty of Science, Benha University, Egypt.

Increasing virazole concentration caused a reduction in microtubers yield (represented in: number of microtubers per plant and microtubers average weight compared with the control treatment (MS medium +80g/l sucrose). The number of microtubers per plant was 0.6 and their average weight was 342.6 mg in case of control treatment, while in case of treatment no. 4(150 mg/l virazole, it was reduced to 0.1 and their average weight was 132.4 mg. Also, there is no effect of Virazole on isozyme pyroxidase activity in potato tissues.

11/42 EFFECT OF SALINITY STRESS ON TUBERIZATION AND POTATO PLANTS GROWTH IN VITRO, CV. SPUNTA.

Mahmoud I. Nasr, Ibrahim A. Ibrahim, Fathy M. El-Shayeb* and

Alaa El-din Sayed Sayed Ewase Khodeir**

Genetic Engineering and Biotechnology Research Institute, Menoufiya

University, Sadat City, Egypt.

*Botany Department, Faculty of Science, Cairo University, Egypt.

**Molecular Biology Unit, Faculty of Science, Benha University, Egypt.

Sodium Chloride with different concentrations (1462, 2924, 5848 and 11696 ppm) had reduced both potato plantlets growth in vitro and production of microtubers, while in case of its concentration (11696 ppm), there were neither roots formed nor microtubers. The growth rate parameters were (9.4 cm, 9.8, 4,7.1 and 8.5 cm) in case of control treatment, while it reached to (1.7 cm, 2.4, 1.4, 0 and 0 respectively) in case of NaCl concentration 11696 ppm., with a significant difference among all treatments. So, increasing NaCl concentration leads to more reduction in plant growth rate, also it leads to a decreasing in the percentage of survivals until 10% in the case of 11696 ppm NaCl, compared with the control treatment (98%), also, in this case there were no roots formed at all. While, in case of the effect of NaCl on production of microtubers, the most important parameters were (0.6 microtuber per plant and an average weight of 342.6 mg in case of control treatment, while they became 0.13 and 24.6 mg respectively in case of NaCl concentration 5848 ppm with a significant difference among all treatments. In case of the highest NaCl concentration (11696 ppm), there were no microtubers formed absolutely in any jar, so, it is clear that NaCl reduced microtuber yield, Also, increasing Sodium Chloride concentration led to an increase in the percentage of shoot tip necrosis with also a significant difference among all treatments.
Scroll to top