Vol. 43, June, 2013.

1/43 GENETIC DIVERSITY AMONG FIVE VARIETIES OF FABA BEANS AND THEIR HYBRIDS AS REFLECTED BY VARIATION IN MORPHOLOGICAL TRAITS AND BIOCHEMICAL MARKERS

Mohamed Akmal El-Kholy, Mahmoud M. Mansour, Abdelghany Sobhy and Abdelfattah Badr*

Botany Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt

* Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo, Egypt

Five faba bean varieties (Sakha 1, Sakha 2, Sakha3, Giza 3 and Giza 843) were hybridized together to produce 10 hybrid lines. Genetic diversity among the parent varieties and their hybrids were then investigated based on variation in morphological traits and polymorphism created by isozymes and protein elctrophoresis. Measurements and observations of morphological traits clearly indicated that hybrids generally have higher values compared to their parents. Higher values of some quantitative vegetative criteria(e.g. plant height) are associated with high yield parameters (e.g. number of pods/plant, number of seeds/pod, and weight of 100 seeds).Considerable variation in seed storage protein and isozymes electrophoretic pattern was found among the five faba bean and their hybrids. These findings can be used to guide future breeding and management of these faba bean genotypes, for improving yield of faba bean as important pulse crop. The analysis of genetic diversity among the five varieties of faba bean and their hybrids using the NTSYS-pc and the CAP software produced UPGMA trees of similar topology. In all trees, the five varieties and their ten hybrids are differentiated into two main groups, one small group represented by Var. Giza 843 (G8) with its two hybrids with Sakha 2 (G8/S2) and Sakha 1 (S1/G8). Second group is represented by Var. G3 with the most of the G3 hybrids (G3, G3/S3, S1/G3 and G3/S2) as well as the two hybrids of Var.S3 with Giza 843 (S3/G8) and Var.Sakha1 (S1/S3).

2/43 PARTIAL CHARACTERIZATION OF A NOVEL BACTERIOCIN

SUBSTANCE PRODUCED BY LACTOBACILLUS SPP.

Abeer Mohamed, Hoda Mahrous*, Mahmoud. AbdEl-Mongy, Ahmed.I. El-Batal**and Hanafy. A. Hamza

Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, Menuofiya University, Egypt.

*Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, Menuofiya University, Egypt.

**National Center For Radiation Research and Technology, Egypt.

Bacteriocins producing Lactobacillus spp. strains isolated from raw milk and Ras cheese, showed broad range of antibacterial activity against some major food borne pathogens.The purification of the bacteriocins produced is helpful for the studies of the mechanism of action, structure, and other characteristics. The bacteriocins had been purified by ammonium sulphate precipitation and dialysis. The recovered proteins were fractionated by using sephadex G-200.The results obtained of different LAB bacteriocins carried out the total bacteriocin activity (AU/ml) from (3200-38000) and a total protein (mg/ml) from (85-171).



3/43 BIOLOGICAL EVALUATION FOR FEEDING ON LOW CALORIES LENTIL SOUPS CONTAINING JERUSALEM ARTICHOKE FLOUR

Al-Anwer M. Osman, Shahat M. Salem,Abul-Fadl M. mohammed

andBadr S. Abdel-Maksoud.

Food Science and Tech. Dept., Faculty of Agric., Al-Azher Univ., Cairo, Egypt

In this research we through spot light onbiological evaluation of low calories lentil soups containing Jerusalem artichoke flour (JAF); as source of inulin at level of 10, 20 and 30%. Feeding of experimental normal and diabetes rats on lentil soup containing 10% JAF (group3), lentil soup containing 20% JAF (group4) and lentil soup containing 30% JAF (group5) for experimental periods(three weeks). Feed intake and body weight gain were recorded daily during the experimental period. At the end of experimental period blood samples were collected to analysis of determination of blood glucose levels, the activity of serum alanine aminotransaminase (ALT) and aspartate aminotransaminase (AST), uric acid, createnine, urea, albumin, total cholesterol, triglyceride, high density lipoprotein cholesterol (HDL) and low density lipoprotein cholesterol (LDL) were determined. The present results cleared that feeding of non-diabetic rats on lentil soup or lentil-Jerusalem artichoke soups was significantly lowered the body weight and feed intake, as compared with that fed on control basal diet (group1). Also significant increase in liver and kidney functions and the HDL cholesterol level, and significant decrease in total cholesterol, triglyceride and the LDL cholesterol level was observed, as compared with control basal diet (group1). On the other hand in case of feeding diabetics rats on lentil diets containing 10,20 and 30% JAF the results showed a significant increase in body weight and HDL cholesterol, and a decrease in feed intake, sugar level, liver and kidney functional parameters, total cholesterol, LDL cholesterol and triglyceride in blood serum of diabetic ratsfeeded with low calories Lentil-Jerusalem artichoke, as compared with basal diet (control, group1) or feeding on lentil soup diet no containing the JAF   (control, group 2). Therefore, the above mentioned results permit and encourage the use of Jerusalem artichoke flour in preparing of some functional foods for human being suffering from diabetic and hypercholestermia.

4/43 Impact of compost tea, Humic acid and microbial inoculant on acclimatization stage of date palm

Abeer H.E. Abd-El Kareim and Gamal A.M. El-Sayed*

Central Laboratory of Date Palm for Res. and Development, (ARC) Giza, Egypt

*Soils, Water and Environment Res. Inst. ARC, Giza, Egypt

Little is known about the effect of organic biofertilizers on date palm acclimatization process. Compost tea, Humic acid and EM at three concentrations (50, 100 and 150 ml/l) and combination between previous concentration of compost tea and Humic acid with 50 ml/l EM were used as fertilizers every two weeks for 12months during acclimatization process of date palm dry cultivar Bartomouda. Plants received NPK at 2g/l were used as control treatment. Morphological parameters were enhanced to high values using compost tea at any concentration in combination with EM at 50ml/l. Application of compost tea (150 ml/l), compost tea (50, 100 or 150 ml/l) + 50 ml/l EM and Humic acid (150 ml/l)+ EM (50 ml/l) enhanced significantly leaves N content while compost tea+ EM (50 ml/l) and chemical fertilizers, EM (150 ml/l) enhanced leaves K content. No significant difference of P content could be observed between all treatments used. Chemical fertilizers and Humic acid succeeded to increase contents of N and K in the soil. On the other respect, compost tea, Humic acid and EM were the superior for increasing the biological activity in the date palm rhizosphere in terms of total counts of bacteria, actinomycetes and fungi as well as dehydrogenase activity.

5/43 FLOW CYTOMETRIC STUDY OF DIFFERENT IMMUNE CELLS TOWARDS CEPHALOSPORIN AND

TACROLIMUS/FK-506

Hussein H. El-sheikh, Mohammed M. Shrief*, Mohy eldin M. El-sheikh**, Mahmmoud M. El-Asser* and Mohammed Y. Mohammed**

Department of Botany and Microbiology, Faculty of science Al-Azhar University

*Department of Microbiology and Immunology, Faculty of Medicine,

Al-Azhar University

**Regional Center of Mycology and Biotechnology, Al-Azhar University

The current study aimed to investigating the effect of cephalosporin and tacrolimus/FK-506 on different human immune cells namely B cells, T cells and monocytes. Flow cytometric analysis revealed the activation/proliferation response of immune cells in untreated cells and conditions treated with different metabolites. Cephalosporin activated immune cells after 24 h treatment, while addition of FK-506 along with cephalosporin antagonizes this effect. Lipopolysacarides with cephalosporin induce proliferation of immune cells (after 4 days treatment), and Fk-506 antagonize effect of cephalosporin providing strong evidence that tested compounds which have been used as commercially drugs in market have immune regulatory effect on human, and this immune regulatory activity can explain immune disturbances in patients chronically treated with these metabolites.
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