Vol. 45, October, 2013.

1/45 STUDY ON THE PLANT DIVERSITY IN SAQQARA REGION, GIZA, EGYPT

Safwat A. Azer

Flora and Phytotaxonomy Researches Department, Horticultural Research

Institute, Agricultural Research Center, Dokki, Giza, Egypt

The aim of this study was to survey the plant diversity among five represented sites; cultivated lands, farm lands, canal banks, water bodies and desert lands in Saqqara region. A total of 89 species belong to 79 genera and 35 families were collected and identified with regional floras and available checklists. The most species families were Gramineae followed by Compositae and Cruciferae.Gramineae was represented by 20 species (22.47 %), while Compositae and Cruciferae were represented by 8 species (8.99%) and 6 species (6.74%), respectively. Four species were recorded from each Chenopodiaceae and Convolvulaceae and three species from each Cyperaceae, Euphorbiaceae, Leguminosae, Polygonaceae and Solanaceae. Moreover, seven families were represented by 2 species and 18 families were represented by one species. On the whole, the annuals species were represented by 53 species (59.56%); perennials ones were 28 (31.46%), while each shrubs and trees species were 4 (4.49%). Based on species distribution among five represented sites, the cluster analysis divided the studied species into four clusters. Cluster one included cultivated and farm lands species. Clusters two, three and four included canal banks, desert lands and water bodies species, respectively. Moreover, the species of cluster two has more similarity to the species of cluster one than species of clusters three and four. Based on the degree of similarity, the species of cluster one have the highest degree of similarity value 0.448 (44.8%), while there was no degree of similarity between species of cluster four and species of clusters one and three, respectively. Further floristic and ecological assessments are needed to conserve this area.

m├ędicaments cialis 20mg 2/45 CULTIVATION AND PRODUCTION OF OYSTER MUSHROOM (PLEUROTUS EOUS AND PLEUROTUS PULMONARIUS) USING SOME AGRO-RESIDUAL SUBSTRATES IN EGYPT

Khaled A. Abdel- Kawi

Soils, Water and Environ. Res. Inst., Agric. Res. Center, Giza, Egypt

Huge amounts of agricultural crop residues were generated in Egypt every year, mainly rice straw and maize stalks. Most of these residues are compose of three major groups of polymers, cellulose, hemicelluloses and lignin. During winter growing season (2010) four types or grains namely sorghum, rice, maize and wheat were used as carriers for spawn preparation. The most suitable spawn was prepared on rice grains. Two strains of quoi pour remplacer le viagra Pleurotus were cultivated on rice straw and maize stalks at spawn ratio 5%. The best biological efficiency (BE %) was 81.5 and 63.0% for cialis 20mg en pharmacie P. eous and information sur viagra P. pulmonarius, respectively on rice straw. Chemical analysis of fruiting bodies showed that P. eous recorded 14.7, 5.65, 58.2, 12.6 and 9.9 % for crude protein, fat, carbohydrate, fiber and ash respectively. Also, chemical analysis of rice straw was carried before and after cultivation.

cialis less side effects 3/45 PREPARATION OF MONOCLONAL ANTIBODIES AGAINST femme et sildenafil NEISSERIA MENINGITIDES cialis 20 generique france TYPE A AND C ANTIGENS

Amanie Mohamed El Barbary, Mohamed Sayed Salama*, Fawkia Mohamed El Beih* and Khaled Zakaria El Boghdadi*

The Egyptian Holding Company for Biological Products and Vaccines (VACSERA). *Faculty of Science, Ain Shams University, Egypt achat viagra pharmacie sans ordonnance .

A system for constructing Monoclonal antibodies (Mabs) against type A and C polysaccharide antigens of chimio et viagra Neisseria meningitides was developed. BALB/c mice were immunized with type A and C polysaccharide capsular antigens from prix sildenafil generique en france Neisseria meningitides. The splenocytes from the immunized mice were fused with P3NS1 myeloma cell line. Hybridoma cell lines producing monoclonal antibodies against type A and C Neisseria meningitides polysaccharide antigens were successfully obtained. These Mabs were characterized and their specificity and isotype were determined. All of the obtained hybridomas were of IgM class. These Mabs were used for developing of Neisseria meningitides diagnostic ELISA kits. The diagnostic specificity and sensitivity of the developed kits were determined.


4/45 PRODUCTION OF POLYMER BY IRRADIATED STREPTOMYCES SP. IN RENEWABLE PLASTIC

Seham M. Salama,Samar Abd-ElFattah El-Gohary and Hussein M. El-Kabbany*

Botany Dept., Faculty of Sciences, Banha University, Banha and *Health Radiation Research Dept., National Center for Radiation Research and Technology. Atomic Energy Authority, Cairo, Egypt.

The Polymer of PHA synthases are the key enzymes of biosynthesis and use the coenzyme A- hydroxy fatty acids as substrates. The two classes of PHA syntheses differ in the specific use of hydroxy fatty acids resulting of the two types: poly (SCL) from hydroxy fatty acids with short chain lengths including three to five carbon atoms, synthesized by actinomycetes, including Stretomyces sp. (PHA) SCL and MCL (short and medium-chain-length) from hydroxy fatty acids, a few actinomycetes synthesize polyester from the above two types. A large scale synthesis can be done with the help of soil organisms. The polymer is a product of carbon assimilation (from glucose or starch) and is employed by microorganisms as a form of energy storage material. Streptomyces sp. are known as producers of several bioactive metabolites which has antibiotic,alkaloid,enzyme inhibitor, immunoactive peptide. Polyhydroxyalkanoates (PHAs) are biodegradable aliphatic polyesters, produced by many common microorganisms and comprising a relatively small amount of other medium chain length. The effect of gamma rays isolates which exposed to various irradiation doses on the production of antibiotic as substances and pigments and also the effect of biomass yield on the investigated actinomycetes especially Streptomyces sp. in the renewable of microorganisms on the surface of plastics followed by the colonization of the exposed surface is the major mechanisms in the microbial degradation of plastics. The enzymatic degradation of plastics by hydrolysis is a two-step process: the enzyme binds to the polymer substrate then subsequently catalyzes a hydrolytic cleavage, polymers are degraded into low molecular weight oligomers and monomers and, finally mineralized to carbon and energy sources.
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