Vol. 49, June, 2015

med france acheter cialis 1/49  STUDIES ON MICROBIOLOGICAL AND PHYSICOCHEMICAL CHANGES DURING WASTES COMPOSTING

Mamdouh S. Elgamal, Ayman Farrag Ahmed, Mohammed M. Afifi*and

Salah Abdelbary

Botany and Microbiology Dep., Faculty of Science, Alazhar University, Cairo, Egypt.

*SWRI, Agric. Res. Center (ARC), Giza, Egypt.

Composting is one of the most successful biological processes for treatment of wastes residues. Microorganism’s activity during composting of waste materials were influenced by initial carbon/nitrogen ratios and physicochemical changes, e.g., electrical conductivity, temperature, pH, organic matter (OM) degradation, and total nitrogen (TN) losses. Compost heap was formed from mixture of rice straw, sugar beet straw and chicken manure. Also, total count of bacteria, fungi, actinomycetes, detection of pathogenic bacteria such as total and faecal coliform bacteria and salmonella were determined. Some physical and chemical analysis of compost during process was determined to determine compost maturity such as phytotoxicity and nitrification index. Thermophilic phase during composting process was decreased numbers of pathogenic microorganisms and after one month disappeared. Also, at the end of process E.C values were decreased after 4 months and germination index recorded 88.1% for maturity of compost.

viagra plusieurs rapports 2/49  MOLECULAR CHARACTERIZATION OF SOME TERMINALIA SPECIES PLANTED IN EGYPT USING RAPD MARKERS

Hany A.M. Mahgoub

Botany andMicrobiologyDeparment, Facultyof Science (Boys),Al-Azhar Univ.Cairo, Egypt

Seven species of the medicinal Terminalia tree planted in Egypt were characterized at molecular level by using random amplified polymorphic DNA (RAPD) markers. viagra bien fait Terminalia species involved acheter viagra au senegal T. arjuna, T. angustifolia, T. bellirica cialis nouvelle indication , mode d'emploi du viagra T. catappa, T. laxiflora, T. mulleri and obtenir ordonnance viagra T. myriocarpa. Ten out of twenty five tested RAPD primers which gave repeatable polymorphic products producing a total of 65 bands, 35 of them were polymorphic. Thepercentage of polymorphism ranged from 25 to 85.71 % and it was amounted to 53.85 % with an average of 6.5 amplified bands per primer. RAPD marker exhibited high level of genetic similarity ranged from 0.6393 to 0.8621 between the studied species. The highest genetic similarity value (0.8621) was recorded between doit on avoir une ordonnance pour acheter du viagra T. mulleri and le generique du propecia T. myriocarpa, whereas, the lowest similarity value (0.6393) was reported among acheter viagra discount T. angustifolia and T. catappa. Genetic relationship between the studied species was determined on the base of polymorphic product analysis and presented in the form of dendrogram. Seven of Terminalia species used in this study were categorized into two major groups, the first group included T. catappa and T. laxiflora, while the second group comprised 5 species viz. T. arjuna, T. angustifolia, T. bellirica, T. mulleri and T. myriocarpa. It was observed that T. angustifolia was close to T. bellirica, reflecting a narrow genetic diversity, whereas T. arjuna was comparatively distant. These results indicated that RAPD is a suitable molecular marker can be used in characterization of genetic relationship among Terminalia species planted in Egypt that could help in their genetic improvement and their breeding programs

3/49  COMPARATIVE BOTANICAL STUDIES ON THREE DIFFERENT TRIBES BELONGING TO POACEAE

Mahmoud A. El-Fiki, Ahmed B.I. Khalil, Fathey M.A. Sabek and

Mahmoud I.A. Lila

Dept. of Agri. Bot., Faculty of Agri., Al-Azhar Univ., Cairo, Egypt

This study is conducted on 21 species that represents 16 genera belonging to 3 tribes: Aveneae, Eragrostideae and Paniceae (Poaceae).The samples are collected from different areas in Egypt. The study is done on the morphological characters of roots, stems, sheaths, leaves, flowers and inflorescences. Also the anatomical characters of roots, stems, leaves and pollen grains characters are studied. The results are recorded in the form of comparison between the examined plants. Most of the examined plants are annual herbs as in Avena sterilis but some are perennial as in Echinochloa pyramidalis. The aerial stems are present in all examined plants, while the rizomatous subterranean stems are present in Desmostachya bipinnata. Pollen grains are monads in all examined plants, with smooth exine in Eragrostis cilianensis and granular exine in Polypogon monspeliensis. The anatomical study shows that the epidermis is simple in all examined plants except in Hoicus annuus it is multiepidermis. From the analysis of the collected results by using the programme (MVSP), we can separate all of Hoicus annuus, Dinebra retroflexa, Eragrostis cilianensis, Panicum maximum and Setaria viridis into one tribe.

4/49  PHYTOCHEMICAL STUDIES oN Some species of Apocynaceae and asclepiadaceae

Ahmed M. Fawzy, *Dalia G. E. Gabr, *Amaal H. Mohamed, *Azza A. F. Khafagi and Fatema S. Mohamed

Flora and Phytotaxonomy Research Dept., Horticultural Research Institute, ARC.

*Botany and MicrobiologyDepartment, Faculty of Science, Al Azhar University (Girls), Cairo, Egypt.

        Taxonomic relationship of seven species of family Apocynaceae and seven species of Asclepiadaceae was analyzed using SDS-PAGE seed protein electrophoresis and fatty acids composition of the seed oil. The total number of protein bands recorded about 19 bands varied from plant to another. Bands ranging between 4– 9 for each species. There were no institute bands in all studied species. The oil extracts of studied species recorded seven saturated fatty acids and six unsaturated fatty acids where oleic acid was the main fatty acid beside linoleic acid and other usual compounds. The relationship between the studied species has been analyzed using cluster analysis of PAST version 2.17. This study support consider the two families as a large family: Apocynaceae and this agree with (Hallier 1912, Demeter 1922, Safwat 1962, Stebbins 1974, Thorne 1992, Judd et al., 1994, Struwe et al., 1994, Sennblad and Bremer 1996, Endress and Bruyns 2000 and APG 2003).


5/49  MOLECULAR AND IMMUNOLOGICAL STUDIES ON A MOSAIC VIRUS NATURALLY-INFECTING HENBANE PLANT

Reham M. El-Baz, El-Sayed T. *Abd El-Salam, Mohamed E. Osman and

Asmaa F. Abd El-Monem

Botany and Microbiology Department, Faculty of Science, Helwan University.

*Botany and Microbiology Department, Faculty of Science, Cairo University.

Egyptian isolate of henbane mosaic potyvirus (HMV) was used for production of HMV specific antiserum and the antiserum titer obtained was 1/16. Immuno-Electron microscope by using prepared antiserum and partial purified HMV was shown a decorated particle.  Total RNA was extracted from infected henbane plant. Using specific oligonucleotide primers, the viral gene was amplified successfully by PCR producing ~778 bp fragment. Comparative nucleotide sequence analysis for HMV showed a similarity of 90.2 % to the HMV isolate (Hungary-isolate) and was published in GenBank with accession number KM497011.

6/49  CAROTENOIDS PRODUCTION FROM RHODOTORULA MUCILAGINOSA  MUTANT AND THEIR APPLICATION AS ANTIOXIDANT AND COLORANT AGENTS IN SWEET CANDY

Shaaban H. Moussa

Genetic Engineering and Biotechnology Research Institute, University of Sadat City, El-Sadat City, Egypt.

Carotenoids are important bioactive colorant with high values. A native yeast strain, Rhodotorula mucilaginosa, was isolated from Qassim region, Saudi Arabia and used for carotenoids production. Dates’ syrup was applied as a substrate for carotenoids production media. The most favorable conditions for yeast carotenoids production were the incubation temperature of 28°C, a pH value of 6.5 and the fortification with 0.5% peptone. The mutant strain RM-5-3-7 was generated from R. mucilaginosa after sequential UV mutagenesis. The mutant strain could grow well in date syrup-based media and its carotenoids production reached 91.62 mg/L with a productivity increase of 427.16% than the parent strain which produced 17.38 mg/L. Yeast carotenoids had strong antioxidant activities and the free radical scavenging activity increased in mutant strain carotenoids than its parent strain. Sweet candy was manufactured and colored with yeast carotenoids and the sensory evaluation of colored candies indicated that concentration of 10mg/kg was the most desirable for candies coloration.

7/49  STUDIES ON A MOSAIC VIRUS NATURALLY- INFECTING HENBANE PLANT

Reham M. El-Baz, El-Sayed T. *Abd El-Salam, Mohamed E. Osman and

Asmaa F. Abd El-Monem

Botany and Microbiology Department, Faculty of Science, Helwan University.

*Botany and Microbiology Department, Faculty of Science, Cairo University.

Egyptian isolate of potyvirus henbane mosaic virus (HMV) was isolated from infected henbane plants collected from the farm of Faculty of Pharmacy, Cairo University in January 2013. The naturally-infected henbane plants exhibited severe mosaic, rugosity, blistering and malformation.  HMV was isolated and identified using symptomatology and diagnostic hosts where it produced symptoms on Chenopodium amaranticolor, Datura stramonium, Nicotiana tabacum cvs. White Burley and Sumsun, N. glutinosa, N. rustica, Solanum demissum and S. tuberosum.  HMV was mechanically transmitted to different species belonging to families Chenopodiaceae & Solanaceae. Thermal inactivation point, dilution end point and longevity in vitro of HMV were found to be 65 ºC, 10-4 and 4 days, respectively. Electron micrograph of partial purified HMV revealed the presence of flexuous filamentous virions with size1440 X14.3nm negatively stained with 2% phosphotungestic acid.

8/49  QUALITATIVE AND  QUALITATIVE INFLUENCE OF BIOFERTILIZERS ON PELARGONIUM GRAVEOLENS L.

Maie M.A. Mohsen, Hassan Ismail*, Abeer H.M. Kasem, Azza M. Abd El-Aal*

Department of Medicinal and Aromatic Plant Researchs, Horticulture Research Institute, ARC .

* Department of Agricultural Microbiology Researches, Soils, Water and Environment Institute, ARC.

        The present study was carried out during the two successive growing seasons of 2011/ 12 and 2012/13 to investigate effects of bio-agent technology. Manipulation of some cyanobacteria like Anabaena azollae or Spirulina platensis or white rot fungi like Pleurotus columbinus, Geranium subtilis under 50, 75 and 100% of N fertilizer on growth, herb yield, essential oil % and essential oil yield and its components. Geranium seedlings were soaking with these bio-agents then plants were sorayed with the bio-agent suspension five times after sowing. Results indicated that inoculation with these bio-agents caused significantly increase in plant height, number of branches/plant, herb fresh and dry weights per plant (g) and per fed (ton), essential oil percentage in herb, and essential oil yield per plant (cc) and per fad (L) as well as Total carbohydrates (%).The highest increase in these parameters obtained when plants treated with Anabaena azollae(A)+ Spirulina platensis(B) + Pleurotus columbinus (C)in the present of 75% of N fertilizer. The lowest mean of all parameters in two cuts for the both seasons were obtained in the plants which fertilized by 50% of N.The highest percentages of Geranyl formate, linalool, citronellol and geraniol in essential oil were recorded with the same treatment. In addition, the highest total carbohydrates percentages and phenol content were recorded in herb of treated plants with this treatment.

9/49  UTILIZATION OF DATES WASTE AS A SUBSTRATE FOR CAROTENOIDS PRODUCTION BY RHODOTORULA MUCILAGINOSA MUTANTS

Shaaban H. Moussa

Genetic Engineering and Biotechnology Research Institute, University of Sadat City, El-Sadat City, Egypt.

The yeast, Rhodotorula mucilaginosa Y23, was isolated from agricultural commodities in Quassim region, KSA, and was characterized with high carotenoids production of 18.58 g/L. Dates syrup (dips) was extracted from inedible date’s fruits (Phoenix dactylifera) and used as a carbon source in fermentation medium. Dips was efficiently utilized by isolated yeasts and R. mucilaginosa Y23 exhibited comparable carotenoids productivity (17.38 g/L) from the date’s syrup-based medium. Consequence UV mutagenesis was applied for the yeast strains and the productivity of mutant strain RM-5-3-3 exceeded its parent strain for total carotenoids productivity with 539.99%. The β-carotene concentration in mutant strain carotenoids increased with 5.64 folds than the parent strain concentration. Dates’ syrup could be, however, recommended as a base for carotenoids production media by Rhodotorula mucilaginosa; the mutagenic treatment with UV is a successful tool to have more powerful carotenoids producing mutants with elevated β-carotene concentrations. Dates’ syrup- based medium is much proper for microbial growth. Rhodotorula  spp. are efficient carotenoids producing yeast. UV mutagenesis could successfully applied to increase carotenoids productivity and β-carotene contents in induced mutants.

10/49  BIOREMEDIATION OF CRUDE OIL CONTAMINATED SOIL BY PHASEOLUS VULGARIS, PSEUDOMONAS AERUGINOSA AND PSEUDOMONAS FLUORESCENS BIOTYPE A

Elshaymaa E. Mussa, Rawheya A. Salah El-Din*, Ahmed Rahal, Lobna A. Moussa and Maie A. El-Khawaga*

*Botany and Microbiology Dept, Fac. Sci., AL-Azhar Univ., Girl Branch, Cairo, Egypt.

A greenhouse experiment was conducted in Agricultural Research Center (A R C), Giza, Egypt to study the reduction of contaminants in crude oil contaminated soil by phytoremediation and microbial degradation (bioaugmentation) using common bean (Phaseolus vulgaris L.) as legume plant,  and Pseudomonas aeruginosa and Pseudomonas fluorescens biotype A, which were previously isolated from Egypt crude oil contaminated soil from Mustorod District beside petroleum companies group, EL-kalyobia Governorate, Egypt. The soil used in this experiment was collected from EL-Ismailia Governorate, Egypt, and was polluted artificially in laboratory by mixing with crude oil at rate 10 mg oil / g soil (1%) before planting .The reduction of residual hydrocarbons in the soil was observed for a period of about 60 days .The study showed that the phytoremediation of crude oil contaminated soil by Phaseolus vulgaris only was not sufficient to degrade the hydrocarbons in soil whereas the addition of biodegraders Pseudomonas aeruginosa and Pseudomonas fluorescens biotype A improved the efficiency of rhizoremediation  and increased the degradation process of crude oil, results referred to Pseudomonas fluorescens biotype A was more effective than  Pseudomonas aeruginosa in the degradation of residual hydrocarbons process.
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