Vol. 30, October, 2008.

ou acheter du viagra pas cher 1/30 ASSESSMENT OF THE POTENTIAL GENOTOXIC RISK AND ANTIGENOTOXIC EFFECT OF CASSIA SENNA L. LEAVES AQUEOUS EXTRACT USING ALLIUM CEPA ROOT TIP ASSAY

Sameer H. Qari

Department of Biology, Teachers College, Umm Al-Qura University,

Makkah, Saudi Arabia.

combien ca coute le viagra Cassia senna L. is widely used as a laxative, although potential side effects, such as toxicity and hepatotoxicity, have been reported. This study evaluated genotoxic and anti-genotoxic effects of vente du cialis en france Cassia senna leaves aqueous extract. So far there is no report on the biological properties of prix sildenafil eg Cassia senna in plant test systems. The Samples were subjected to cytogenetic and molecular genetics assay by Chromosomal aberration & micronuclei test; DNA & protein quantity, RAPD profile & SDS-PAGE protein profile in root cells of kamagra mode d'emploi Allium cepa. Our results demonstrated that leaves extract of over the counter viagra germany Cassia senna produces chromosomal aberrations specially in micronuclei formation at high concentration, whereas low concentrations reduced the genotoxic effects of ethyl-methane-sulfonate. On the other hand, the extract decreased DNA and protein quantity at concentrations higher than 5%, whereas the low concentrations were not effective on DNA or proteins quantity. The RAPD and SDS-PAGE results demonstrated polymorphic bands by the treatments with 5, 15, 50% of peut on acheter du viagra a la pharmacie sans ordonnance Cassia senna leaves extract compared with the control, whereas the treatments with the concentrations of 0.5, 2% did not induce any change in RAPD and protein bands, at the same time these treatments reduced the polymorphic bands, which were occurred by ethyl-methane-sulfonate. All these results strongly suggest that the leaves extract of effet secondaire du sildenafil Cassia sennais a clastogenic and mutagenic at higher dosage and antigenotoxic at lower doses.

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2/30 PRODUCTION OF CLEAR PEACH JUICE BY ENZYMATIC TREATMENT: A PRELIMINARY STUDY ON THE PROPERTIES AND STORAGE STABILITY

El-Sayed S. Abd-EL-Wahab, Korany H .Tolba and Abd El-Rahman M. Sulieman*

Food Technology Research Institute, Agriculture Research Center (ARC),

Giza, Egypt.

*Department of Food Science, Faculty of Agriculture, Zagazig University,

Zagazig, Egypt.

Owing to the difficulty of producing peach juice from peach puree, the current study was carried out to produce clear peach juice by treated peach puree with different concentration from commercial enzymes (pectinex and cellubrix) and evaluation of some quality parameters of clear single strength peach juice and juice concentrate during storage at different condition. The results show that total soluble solids and total sugar content of peach puree were 11.8% and 9.26 %, respectively and viscosity of peach puree was 52.4 mPas. Enzymatic treatment with pectinex caused obviously decrease of peach puree viscosity than treatment with cellubrix. The highest viscosity loss percent (77.77%) and highly increase of peach juice clarity (94% transmission) were obtained with treatment by a mixture of 250 ppm pectinex and 200 ppm cellubrix. This treatment also caused slightly increasing of Brix degree, total sugars and reducing sugar contents of peach juice. The changes of physico-chemical and sensory properties of clear single strength peach juice and its concentrate were very limited during storage especially storage at 5oC±1oC. The results of sensory evaluation indicated that the color, taste and aroma of single strength peach juice stored at 5oC±1oC were better than stored at room temperature. Finally it is possible to produce clear peach juice characterized with high quality using the mixture of pectinex and cellubrix enzymes.

 

3/30 PROTECTIVE EFFECTS OF ERYTHRITOL PRODUCED BY YEAST OF MONILIELLA TOMENTOSA VAR POLLINISAGAINST STREPTOZOTOCIN-INDUCED DIABETES IN RATS

Osman M. Al-Anwer, Mostafa A. mohammed,Bader S. Abdel-Maksoud,

Nabila S. Hassan* andMosaad A. Abdel-Wahhab**

Food Science and Tech. Faculty of Agric., Al-Azher Univ., Cairo, Egypt

*Pathology Department and **Food Toxicology & Contaminants Department, NRC, Dokki, Cairo, Egypt

Non-digestible carbohydrates (NDC) like sweetness are well known to possess interesting functional and physiological attributes, non-carcinogenicity, low caloric value, prebiotic, hypolipidemic and hypocholestrolemic properties. The aim of the current study was to evaluate the erythritol produced by yeast against diabetes induced by streptozotocin (STZ) and its complications in rats. Twenty-four rats were divided into 4 treatment groups including the control group, the diabetic group, the diabetic rats groups received low dose (100 mg/kg b.w) and high dose of erythritol (400 mg/kg b.w) for 3 weeks. The results indicated that STZ induced biochemical changes in the serum and histological changes in the liver and pancreas typical to those reported in the literature. Erythritol administration showed significant improvement in all the biochemical parameters tested as well as the histological picture of the liver and pancreas. Moreover, these improvements were dose dependent and erythritol itself was safe even at the high tested dose.  

4/30 EFFECT OF PLANT GROWTH REGULATORS AND POLYETHYLENE GLYCOL ON MATURATION OF DATE PALM SOMATIC EMBRYOS

Zeinab E. Zaid., *Adel A. Abul-Soad and Rehab A. Sidky

The Central Laboratory for Date Palm Researches and Development, Agriculture Research Center, Egypt

*Department of Tropical Fruit, Horticulture Institute, Agriculture

Research Center, Egypt

This study aim touseplant growth regulators and PEG to enhance embryo maturation and obtain a vigorous plantlets of Phoenix dactylifera L. cv. Malakaby. Embryonic nodular callus 0.5 g were treated with1 µM ABA or JA with BAP or NAA and PEG at 0 or 0.25%. Addition 1µm ABAintomaturation medium was more effective to produce somatic embryo numbers (11.60) compared to 1µm JA(6.11). Addition PEG into maturation medium did not enhance significantly somatic embryo number (10.33 and 7.38 respectively). The embryos length increased by addition ABA into maturation medium compared to JA as well as, addition BA with ABA into maturation medium encouraged embryos length. Osmolarity affected by PEG seemed the main component required for protein synthesis. Furthermore, the plantlets which treated with PEG treatments appeared vigor and healthy compared to the plantlets which untreated with PEG.

5/30 OPTIMIZATION, CHARACTERIZATION AND APPLICATION OF LIPIDS EXTRACTED FROM PYTHIUM DEBARYANUM

Hanan M. Mubarak and Eman H.F. Abd El –Zaher

Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.

Production of lipids by Pythium debaryanum was achieved by growing the fungus on Czapek's Dox medium (pH 6.5) for 8 days at 300C, using glucose as a carbon source and NaNO3 as a nitrogen source in the culture media and exposing the growth of the fungus to UV radiation for 80 minutes. Lipid material extracted from Pythium debaryanum was analyzed by using elemental analysis, gas liquid chromatography, infrared and ultraviolet spectra. Lipids extracted from Pythium debaryanum was effective against the following microorganisms (Candida albicans, Cladosporium cladosporioides, Penicillium digitatum and Trichoderma viride), gram negative bacteria (Esherichia coli) and gram positive bacteria (Bacillus subtilis).  

 

6/30 HUNTING OF SOME DIFFERENTIALLY EXPRESSED GENES UNDER SALT STRESS IN WHEAT

Clara R. Azzam, Sameer H. Abdelaziz* and Omar S. Ebshish**

Field crop research Institute, Agric. Research Center, Egypt

*Botany department, Fac. Science, Benha University, Egypt

**Biology department, Faculty of science, Al-Margeb University, Libya.

Two wheat cultivars were grown under salt stress at two concentrations (0 and 6000ppm NaCl). RNA of the two examined cultivars were extracted, then reverse transcribed using poly T primer as anchor and RT enzyme to produce 1st strand of cDNA. The produced cDNA were used as a template for PCR reaction mix to perform differential display. The results of differential display showed several modifications in gene expression under salt stress PCR produced 41 amplified fragments with different lengths using anchor primer A. From these fragments, bands 12, 15, 20, 25 were upstream regulated under salt stress where they were denser in stain. On the other hand, band 2 was newly synthesized under salt stress and band 34 was down regulated in both wheat cultivars. The results of PCR products using the same poly T anchor ended with C showed a total of 40 amplified fragments with different lengths. Some of these fragments indicated modifications in gene expression under salt stress as compared to control conditions, one of these is band 2 upstream regulated under salt stress. On the other hand, band 23 was newly synthesized under salt stress in both of the two cultivars, while bands 33, 35 were present in the plants grown under control condition but not in salt treated plants down regulated of the two cultivars. Also the results of differential display using poly T anchor ended with G showed 39 amplified fragments and bands 4 and 34 were present only in control conditions but it was absent under salt stress in both cultivars (down-regulated) as response for salt stress.

7/30 Production variables and intestinal microflora of HY-line layers fed active live yeast-supplemented diets

Saadia M. Hassanein and Naglaa K. Soliman*

Microbiology Department, Faculty of Science and *Poultry Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

As experiment was conducted to evaluate the affect of adding various levels of a live yeast to laying hen diets on their laying and feeding performance, egg shell, egg components and some blood constituents, as well as the intestinal microflora make-up. This were studied to validate the mode of a live yeast action in improving laying hens performance. For this purpose 75 Hy line (W-36) white layers were sited from 70 to 79 week of age in individual cages and randomly distributed into five experimental groups of 15 layers each. The individual hen was represented as an experimental unit. The five experimental groups were fed on five graded levels of a live yeast as 0.0% (control), 0.4%, 0.8%, 1.2% and 1.6%. The main results indicated an increase in egg production percentage of layers fed with 0.4% and 0.8% a live yeast which recorded 83.4% and 80.6% respectively compared with 74% of control which was similar to the groups of layers fed 1.2% (74.9%) and 1.6% (74.6%).   Average egg weight was not influenced by adding yeast into diets. Egg mass results were parallel to these of egg production where the values of 46.7, 51.0, 50.2, 48.3 and 46.1 g egg/hen/day were recorded for the group of birds fed with 0%, 0.4%, 0.8%, 1.2% and 1.6% a live yeast respectively. Egg albumen and egg yolk were affected significantly. There was a slight improvement in egg shell thickness and percentage. Feed intake values were approximately similar within the different treatments. Feed conversion ratios (g feed/g egg) of layers fed yeast levels of 0.4% (2.08) and 0.8% (2.07) were better than the control group (2.27). Blood total protein levels of birds fed 0.4% (3.82), 0.8% (3.65) and 1.2% (3.97) yeast were lower than the control (4.16), while the value of 1.6% yeast (4.16) was slightly higher than control. Blood albumen levels were parallel to those of blood protein while blood globulin values were not affected. Blood cholesterol levels of layers fed yeast-supplemented diets were lower than the control. Blood total lipids were not affected by treatments. Ileal content pH of layers fed 0.8% and 1.2% yeast levels was lower than the control. Microbiological examination of ileal content indicated an obvious reduction in bacterial total count. While Lactobacilli bacterial count was increased. There were reductions in bacterial strains of Escherichia coli (E.coli), Klebsiella sp., Staphylococcus sp., Micrococcus sp., Campylobacter sp., and Closterdium perfringers of layers fed various yeast levels. The results of this study suggest adding live yeast at 0.4% or 0.8% into laying hen diets can enhance the productive performance and nutrients utilization via the inhibitory effect of yeast against pathogenic bacteria.  

8/30 DEGRADATION OF RICE STRAW BY ANAEROBIC FUNGI

Ahmed M. Ragab; Magda A. El-Meleigy; Abd El-Razak A. Abo-Sadaa*

and Mona S. Ashoor**

Botany and Microbiology Departments, Faculty of Science, Al-Azhar University (Girls branch) and *Faculty of Science, Al-Azhar University (Boys), Cairo, Egypt.

**Regional Center for Mycology and Biotechnology (RCMB) at Al-Azhar University

The anaerobic fungi Neocallimastix frontalis and Orpinomyces joyonii were previously isolated from feces of cattle and sheep. Aspergillus flavus; Aspergillus terreus and Absidia corymbiferawere isolated from deep soil samples, they tolerate anaerobic stress. Anaerobic fungal isolates grew well and degraded rice straw under anaerobic conditions producing palmitic and oleic acids while, facultative anaerobic fungal isolates degraded rice straw under aerobic and anaerobic conditions produced many compounds. These fungal isolates could be used in bioremediation processes and in animal food processing.

9/30 ALUMINUM TOXICITY INDUCED CHANGES IN PHOTOSYNTHETIC PIGMENTS AND OXIDATIVE METABOLISM OF ZEA MAYS L. PLANTS

S.S. Abu-Muriefah

Botany Department (Science Section), Girls College of Education,

P.O. Box 27104, Riyadh 11417, Saudi Arabia

The present study was undertaken to assess the toxicity of Al3+ on photosynthetic pigments, total soluble protein and MDA contents as well as the activities of SOD, CAT and POD in Zea mays leaves after 6-days of supplying aluminum in the nutrient solution. The results indicated that aluminum stress treatment adversely affected plant growth and disturbed the cell metabolism seriously. The development of toxic symptoms, corresponded to a high accumulation of Al3+, were due to the increase in MDA content, to the decrease in soluble protein contents and to the much elevated SOD and POD activities in leaves. In addition, the results demonstrated that exposure to high concentration of aluminum (Al3+ > 20 ppm) could result the disintegration of antioxidant system in maize seedlings. Also, the significant decrease in the contents of photosynthetic pigments was related to high-level metal stress. The outcome of this study corroborate that Zea mays is a suitable candidate for the phytoremediation of low-level aluminum contaminated soil.

10/30 PURIFICATION AND CHARACTERIZATION OF AN EXTRACELLULAR POLYGALACTURONASE FROM SCLEROTIUM ROLFSII SACC.

Aisha M.H. Al-Rajhi

Collage of Training Teachers/Riyadh Saudi Arabia

An extracellular polygalacturonase (E.C. 3.2.1.15) was purified upt to 123.22 fold from the culture filtrate of Sclerotium rolfsii Sacc. by dialysis, precipitation with 0.7 saturation ammonium sulfate, gel filtration through Sephadex G-75 and ion-exchange chromatography on diethylaminoethyl cellulose with a yield of 57.79% and specific activity of 32.903 units. mg-1 protein. The purified enzyme exhibited maximal activity at pH 4.5 at 30oC, and, was stable in the pH range of 3.5 to 5.0 and at temperature up to 30oC. Km of the enzyme was calculated to be 3.8 mg ml-1. The molecular weight was determined by SDS-polyacrylamide gel electrophoresis to be 51 kDa. Participation of SH-groups in the catalytic sites of the enzyme is confirmed. Quantitative estimation of amino acids in the purified proteins obtained from the culture of S.rolfsii revealed that, it contained 17 amino acids and the proteins were rich with the aromatic amino acids; phenylalanine and tyrosine (41.6% of the total amino acids). Acidic amino acids, aspartic and glutamic (31.02% of the total amino acids). However, glycine is present in abstemious proportion (2.2% of the total amino acids).

11/30 DETERMINATION OF ANTAGONISTIC ACTIVITY OF SOME TRICHODERMA SPP. AGAINST SOME ROOT ROT FUNGI

Ilham M. El-Refai, Susan M.W. Assawah, Saida M. Amer and Mohamed S. Draz

Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.

In the present study, thirteen isolates of genus Trichoderma belonging to seven species were isolated from Delta region of Egypt and identified. Trichoderma koningii Oud. (T13, T26 and T84) and T. hamatum Bon (T29, T45 and T58) recorded the highest occurring remark. All tested Trichoderma isolates demonstrated high antagonistic action against Rhizoctonia solani Kühn and Sclerotium rolfsii Sacc. in dual culture test. The isolates of T. viride (T28), T. ressei (T25) and T. pseudokoningii (T79) were the most aggressive against R. solani, whereas T. harzianum (T75), T. lignorum (T12) and T. hamatum (T58) were the most inhibitor to S. rolfsii. Interaction between Trichoderma isolates and the tested rot fungi (R. solani and S. rolfsii) was documented through light microscopy. The frequency of coiling and penetration interaction of the tested Trichoderma isolates with S. rolfsii was more than R. solani. Trichoderma hamatum (T58) and T. harzianum (T61 & T75) produced both coils and penetration against hyphae of R. solani while T. koningii (T26 and T84), T. hamatum (T29), T. harzianum (T61) and T. pseudokoningii (T79) produced both coils and penetration against hyphae of S. rolfsii. A comparison between the inhibitory effects of volatile and non-volatile metabolites of our Trichoderma isolates on linear growth of R. solani and S. rolfsii revealed that the non-volatile seemed to be more effective. The non-volatile metabolites produced by T. viride (T27) were the most inhibitory (82.22%) to the growth of R. solani, followed by T. lignorum (T12) and T. ressei (T25) which recorded growth inhibition 77.78 %. While, the non-volatile metabolites produced by T. koningii (T13 & T26) and T. pseudokoningii (T79) caused complete growth inhibition of S. rolfsii. In the meanwhile, the released volatile compounds inhibited the vigorous growth of S. rolfsii, while these metabolites exhibited weak inhibitory action on R. solani linear growth achieving growth inhibition percentages lower than 40%.

 

12/30 GENOTYPING ANALYSIS FOR SOME WHEAT CULTIVARS USING INTER SIMPLE SEQUANCE REPEATS

Omar S. Ebshish and Sameer H. Abdelaziz*

Biology department, Faculty of science, Al-Margeb University, Libya.

*Botany department, Faculty of science, Benha University, Egypt.

Eight wheat cultivars namely, Sakha85, Sakha88, Sakha89, Giza115, Giza156, Giza161, Giza163 and Giza168 (hexaploid) were grown up to the seed maturity stage in a randomized complete block design with four replications during the two successive seasons 2006-2007. Traits measured were plant height (cm), number of tillers/plant, number of leaves/plant,Weight of the main panicle and Shoot fresh weight. The obtained data were statistically analysis of the obtained data showed that the highest and the lowest cultivars in each of the measured traits. ISSR-PCR analysis was performed using ten primers to obtain markers to assist selection for some of the aforementioned traits in wheat and some cultivar specific markers. Primers HB1, 814A, 17889B, 17899A and 17899B produced ISSR fragments with different lengths could be used as markers to assist selection for some of the yield-related traits in wheat. Similarity index and the consensus tree were developed based on the ISSR-banding patterns of the eight cultivars. The most two closely related cultivars were Sakha88 and Giza163 with the highest similarity index (0.89) and the most two distantly related cultivars were Sakha88 and Giza156 with low similarity index (0.73). The consensus tree was divided into two main clusters, the first included cultivars Sakha88, Giza163, Giza115 and Giza161. The second main cluster includes cultivars Sakha85, Sakha89, Giza156 and Giza168.

13/30 APPLICATION OF STATISTICALLY-BASED EXPERIMENTAL DESIGNS FOR OPTIMIZATION OF POLYUNSATURATED FATTY ACIDS PRODUCTION BY THE CYANOBACTERIUM SPIRULINA PLATENSIS

Mervat F. El-Sherif

Biological Department, Faculty of Education, Alexandria University,

Alexandria, Egypt

Statistically based experimental designs were applied to the medium components for optimization of polyunsaturated fatty acids production by the cyanobacterium Spirulina platensis. The Placket-Burman and (2n) factorial designs were used to evaluate the effect of variables including medium components on production of polyunsaturated fatty acids. Among these variables CaCl2 was identified to have the significant effect. However, the statistical analysis of the (2n) factorial experiment proved that NaNO3 and micronutrients (a) at 0.25 value recorded in Zarrouk medium gave the highest values of polyunsaturated fatty acids in Spirulina platensis specially C18:2 and C22:6. The total polyunsaturated fatty acids increased by 409.69% (5.07 fold) compared to the original medium.

14/30 ALTERATION IN GROWTH AND THYLAKOID MEMBRANE LIPID COMPOSITION OF AZOLLA CAROLINIANA UNDER PHOSPHATE DEFICIENCY

Ghada Saber M. Ismail and Hala Ezzat Mohamed

Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.

The changes in the fresh biomass accumulation, photosynthetic and anthocyanin pigments, photosynthetic activity (PSII), ultrastructure of chloroplast, total lipids and fatty acid composition of thylakoid membrane were followed in the aquatic fern Azolla caroliniana grown on medium either deficient or supplemented with various phosphorus concentrations. The total photosynthetic pigments and the anthocyanin/chlorophylls ratio increased significantly with increasing PO43- concentration. Phosphate deficiency had a significant inhibitory effect on the growth and PSII activity of A. caroliniana plants, and resulted in a significant decrease in both total and phospholipids in isolated thylakoids. This was accompanied with a significant increase in the percentage of glalactolipids relative to the total lipids.

 

15/30 STEM ANATOMY OF TEN TAXA OF MYRTACEAE IN EGYPT

Wael T. Kasem

Botany and Microbiology Department, Faculty of Science, Al Azhar University,

Nasr City, Cairo, Egypt.

Ten taxa representing 7 genera of Myrtaceae were collected from 7 botanic gardens in Egypt. These studied taxa were Callistemon lanceolatus, Callistemon rigidus, Feijoa sellowiana, Eugenia uniflora, Melaleuca armillaris, Melaleuca leucadendra, Myrtus communis, Pimenta racemosa, Pimenta officianalis, Psidium guajava L. cv. Pear shaped. Stem anatomy of these taxa was described and analyzed by light microscope. My results were compared with classification systems declared by previous researchers. Twenty-one binary characters in the present study were subjected to numerical analysis. The obtained dendrogram classified the studied taxa into two groups The 1st group included 6 taxa namely Feijoa sellowiana, Eugenia uniflora, Psidium guajava, Myrtus communis & two species of Pimenta. The 2nd group included a taxa of tribe Leptospermeae (Callistemon & Melaleuca) in a separate level (25.0%& 30.0%, respectively). The present study suggested that Feijoa sellwiana (as for as i know, not treated by previous studies) was more related to tribe Myrteae.

 

16/30 PURIFICATION, CHARACTERIZATION OF BACTERIAL POLYGALACTURONASE (PGASE) AND ITS APPLICATION IN CLARIFICATION OF GUAVA JUICE

Rada A. Bayoumi; Hesham M. Yassin*; Mahmoud A. Swelim*

and Ebtsam Z. Abdel-All*

Botany & Microbiology Department, Faculty of Science, Al-Azhar University,

Cairo, Egypt.

*Botany Brom

Department, Faculty of Science, Benha University, Egypt.

Bacillus firmus –I-10104 produced very high level of polygalacturonase by solid state fermentation (SSF). Fifty one bacterial isolates were isolated from fermented clayed Solanum tuberosum peels collected from different restaurants in Kalubeia governorate, Egypt. All bacterial isolates were screened for their abilities to produce pectinases using apple pectin under solid state fermentation (SSF) conditions. All of these bacterial isolates were found to have appreciable pectinolytic productivities of which twenty isolates showed good pectinase(s)-producing potentialities using agro-industrial wastes viz. Solanum tuberosum (ST), Solanum melanogenum (SM), Eichhornia crassipes (EC) and citrus peels mixture (CPM) at 30°C and pH 6 by pectin clearing zone (PCZ) technique. Three bacterial isolates, viz: 4071, 107 and 10104 were found to exhibit a higher polygalacturonase (PG) production by attacking Solanum tuberosum (ST) peels as compared to other wastes. The three most potent bacterial isolates were suggestive to identify on the bases of cell shape, cell arrangement, relation to oxygen and physiological and biochemical tests as Bacillus firmus, I-4071, B. firmus-I-10104 and Bacillus laterosporus-I-107. The optimum inoculum size for production of polygalacturonase (PGase) by B. firmus-I-10104 on Solanum tuberosum (ST) peels was 1 ml (30 x 1015 CFU); substrate concentration, 1.25 g/25 ml; incubation period, 96 hours, pH, 6.0; incubation temperature, 37°C; different nitrogen source; peptone (0.1 g/l); different carbon source, control; different amine acids, control and finally without any vitamins. The polygalacturonase was purified by column chromatography on sephadex-G200 and the confirmation of its purity was determined by gel electrophoresis. Two active peaks were obtained and two bands detected in gel electrophoresis. The optimal conditions for maximum activity of purified PGase were: incubation temperature, 50°C; pectin concentration, 2 %; pH, 12; enzyme concentration, 0.5 ml; thermostability, 80°C and pH stability, 12. The partially purified PG that has been applied for clarification of cloudy Guava juice was carried out at pectin concentration 1.5 %; enzyme concentration, 4.5 ml, temperature, 75°C and pH 5. This study indicated the possibility for use Bacillus firmus-I-10104 in the production of polygalacturonase under the all ideal factors by using ST peels as the best agro-industrial waste under SSF conditions and its application for production of clear juices.

17/30 PHYSIOLOGICAL STUDIES OF CELLULASE COMPLEX ENZYMES OF ASPERGILLUS ORYZAE AND CHARACTERIZATION OF CARBOXYMETHYL CELLULASE

Ghada A. Youssef

Botany Department (Microbiology), Faculty of Science, Alexandria University, Alexandria, Egypt

The effect of incubation period, substrate concentration, initial pH and temperature on the production of endoglucanase, exoglucanase and cellulase (filler paper activity FPA) activities, were investigated. Seven days incubation period with an initial pH value of 6 and 40oC temperature. The cheese whey concentration of 10% yielded the highest specific activities with 1.5 fold increases for endoglucanase and 1.4 fold increases for exoglucanase. Cellulase specific activity represented as FPA showed no fold increase at all. The optimum temperature of endoglucanase was 55oC, it was found that the enzyme activity has abroad pH range between 3.8 – 8.0. 24.91% of the original activity was retained after heat treatment at 90oC for 30min. Analysis of the partial purified enzyme preparation by SDS – PAGE revealed one protein band showing cellulolytic activity, the molecular weight of this band was estimated to be around 45.000 Daltons. The aim of the work was producing a cellulose complex enzymes by Aspergillus oryzae on cheese whey as the source of carbon.

 

 

18/30 THE PRELIMINARY   PHARMACOLOGICAL EVALUATION OF NOVEL ASPIRIN DERIVATIVE COMPOUND FOR ANTI-INFLAMMATORY ACTIVITY

S.A. Al-Mikhlafi, Abdulla Al-Thobhani* and Hussein Alkadi**

Department of Medicinal Chemistry, Faculty of Pharmacy, Sana'a University.

*Department of Pathology,   **Department of Pharmacology, Faculty of Medicine and Health Science, Sana'a University.

The objective of this work was evaluation of thepreliminarypharmacological activity of novel Nonsteroidal anti-inflammatory compound, N – [5 – Methyl – 2 – (1, 3, 4 – Thiadiazole)] –Salicylamide, that was designed and prepared as potential selective cox-2 inhibitor. the chronic and acute anti-inflammatory activities, were performed by induced granuloma, while, predicted COX-2 selectivity of tested compound was achieved by   utilizing indirect way by study the ability of this compound to induce ulcerogenic changes in the gastric mucosa, then confirmed by “Haematoxylline eosin” histological section. The results of this study indicate that the prepared compound has well defined anti-inflammatory activity with suspected selectivity toward COX–2 enzyme inhibition within the limit of concentrations used .The over all studies were compared by reference anti-inflammatory drugs (Indomethacin, Rofecoxib, and Meloxicam)

 

19/30 the microbial quality of camel’s milk feeded with Atriplex halimus

Mostafa M. Ashour; Atia A. Abd El-Baky; Mervet M. Mokhtar*;

El-Sayed M. Abd El-Wahed and Alaa H. Ibrahim*

Department of food science, Faculty of agriculture, Zagazig University, Egypt.

*Department of Animal and Poultry Breeding, Desert Research Center, cairo, Egypt.

This study showed the effect of lactation months and feed with Atriplex halimus on the microbiological quality and chemical composition of raw camel’s milk. Results indicated a significant differences (p<0.5) between lactation months and milk chemical composition (total solids, total protein, lactose, fat and ash). The use of Atriplex halimus increased (p< 0.05) the total solids, total proteins and lactose. Also, the results indicated that the total aerobic mesophilic bacterial count, proteolytic bacteria, lipolytic bacteria, enterobacterial group, yeasts, and moulds increased progressively throughout lactation season and reaches the higher mean values in October and November for total aerobic mesophilic bacterial count. while proteolytic bacteria recorded, the highest mean values were obtained in October and December. on the other hand, the lipolytic bacteria, yeast and moulds recorded the highest mean values in October. while the highest mean values for enterobacterial group were recorded in September and October with significant differences (p<0.05) between total aerobic mesophilic bacterial count, proteolytic bacteria, lipolytic bacteria, enterobacterial group and yeasts and moulds.

20/30 SHELF LIFE AND QUALITY ASSESSMENT OF CAMEL’S MILK

Mostafa M. Ashour; Atia A. Abd El-Baky; Mervet M. Mokhtar*;

El-Sayed M. Abd El-Wahed and Alaa H. Ibrahim*

Department of food science, Faculty of agriculture, Zagazig University, Egypt.

*Department of Animal and Poultry Breeding, Desert Research Center, cairo, Egypt.

The results indicated that the raw camel milk had higher shelf life in compared with cow’s milk and spoiled after 15 days when the 20% of camel’s milk was mixed with 80% of cow’s milk, the shelf life of cow’s milk was 5 days with slow growth rate for total aerobic mesophilic bacteria, enterobacterial group and psychrotrophic bacteria logcfu/mlduring storage 5±1°C. While camel’s milk treated with HTST pasteurization had the highest shelf life and spoiled after 28 days with slow development for acidity when compared with 20% raw cow mixed milk (T5) it was 12 days. In case of LTLT pasteurization of camel’s milk it had the highest shelf life and spoiled after 32 days with slow growth rate for total aerobic mesophilic bacteria, enterobacterial group and psychrotrophic bacteria logcfu/ml during storage 5±1°C for the acidity was slow when compared with 20% cow’s milk mixed with camel’s milk (T5) it was spoiled after 14 days.  


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