Vol. 35, June, 2010.

cherche de viagra 1/35 CALLUS INDUCTION FROM IMMATURE EMBRYO, COTYLEDON AND LEAF EXPLANTS OF NEEM (AZADIRACHTA INDICA)

Fawzia Abo-Sria Ebad; Ahmed Ahmed Ahmed Elkazzaz*; Mohamed Ahmed Mattar* and Marwa El-Sebai Abd Elsadek El-Sebai

Botany and Microbiology Department, Faculty of Science (girls), Al-Azhar University, Cairo, Egypt.

*Plant Biotechnology Department, National Research Centre, Cairo, Egypt.

Neem is a fascinating tree. It seems to be one of the most promising plants and may eventually benefits every person on the planet. Neem trees and its seeds do not grow well under moderate climate such as Egyptian climate. So the purpose of the present study was to apply that practically via plant biotechnology. The effects of various MS media on callus initiation from immature embryo, cotyledon and leaf explants referred that the usage of low concentrations of plant growth regulators was better for callus initiation than the higher concentrations. Callus better developed and proliferated on MSII medium containing 0.5 mg/l BAP + 1mg/l NAA and considered the best medium for proliferating and developing the callus growth from the various explants. Elimination of NAA from initiation and developing callus media recorded negative response with all explants except those initiated from leaf explants (28.85 % of cultures), giving low positive effect of callus development and proliferation. It's remarkably that the combination of NAA as auxin with BAP as a cytokinin is required for callus proliferation, development and mass production. CH did not affect the callus proliferation and development.

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effet secondaire viagra alcool 11/35 RESPONSE OF POLIANTHES TUBEROSA, L. PLANT TO DIFFERENT GROWING MEDIA AND GA3 TREATMENTS

Mohamed M. Abdel-Sattar, Samira S. Ahmed and Ali Nabih

Horticulture Research Institute, Agriculture Research Center, Giza

In a trial to success cultivating of cialis en pharmacie en france Polianthes tuberose, L. plant in sandy soil, and obtain high yield and quality of spikes and bulbs, this investigation was consummated under full sun at a nursery of Hort. Res. Inst., Giza, Egypt during 2008/09 and 2009/10 seasons, as the bulbs (7-8 cm circumference) were planted in 25-cm-diameter clay pots filled with about 3 kg of one of the following media: clay (as a control), and a mixture of either sand+compost or sand + sewage sludge, each at a ratio of 3:1, by volume. Gibberellic acid (GA3) solution was also sprayed on the foliage, 4 times with 21 days interval at the concentrations of:0, 50, 100 and 200 ppm, individually or combining with 3 previous media. The obtained results exhibited that planting in sand + compost mixture induced a clear prevalence in most vegetative and flowering measurements, besides precocity of flowering and producing higher quality and quality of bulbs and bulblets at the end of the season, whereas planting in sand + sewage sludge mixture led to increasing the content of N, P, K, amino acids and total, reduced and non-reduced sugars in the new-formed bulbs. This medium, however caused a reduction in the content of phenols in these new bulbs. Regarding GA3 treatments, they led, especially at 50 and 100 ppm concentrations, to a pronounced precocity in flowering, but caused a slight decrement in flowering stalk length and bulbs yield. The concentration of 100 ppm is the only one that increased the fresh weight of the new bulbs. On the other hand, all GA3 concentrations slightly reduced the content of N, P, K, amino acid ant total, reduced and non-reduced sugars in the new bulbs, but raised its content of phenols. The interactions between the used media and the various concentrations of GA3 induced an additional improvement in all vegetative and flowering traits, as well as the yield of bulbs and bulblets/plot, within the superiority of a combination of planting in sand + compost mixture plus spraying with GA3 at 200 ppm level. From the previous results, it could be recommended to cultivating acheter levitra paypal Polianthes tuberose, L. bulbs in a mixture of sand + compost (3:1, v/v) and spraying the foliage, 4 times with 21 days interval, with GA3 solution at 200 ppm to score the best growth, flowering, bulb productivity and lasting flowering duration to late summer and early autumn.

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utilisation du cialis 5mg 2/35 RESPONSES OF SOME FILAMENTOUS FUNGI TO ALKALINE STRESS

achat kamagra jelly I- ISOLATION, IDENTIFICATION, MORPHOLOGICAL FEATURES AND ULTRASTRUCTURE OF SOME ALKALOPHILIC FUNGI

Magda M. El-Meleigy*; Sanaa A. Ahmed; Ebtsam N. Hoseiny and Shadia M. Sabry

Botany and Microbiology Department, Faculty of Science (For Girls), Al-Azhar University, Cairo Egypt

acheter viagra en toute securité Arthrobotrys oligospora, and Aspergillus flavus were isolated from Egyptian soil from Cairo and Sinai at alkaline conditions. They tolerated hydrogen ion up to 12 and 11.5 pH (respectively) and temperature up to 40oC.Their response to alkaline conditions was determined .The metamorphoses on morphology were discovered at different pH values and some ultrastructure too.

 

3/35 EUKARYOTIC ACIDOPHILES

I- ISOLATION, IDENTIFICATION AND SOME PHYSIOLOGICAL STUDIES

Magdh A. El-Meleigy, Ebtsam N. Hoseiny and Monira O. Zhran

Botany and Microbiology Department, Faculty of Science (For Girls), Al-Azhar University, Cairo, Egypt

Three acidophilc fungal isolates were isolated from water and soil of Ain Helwan and Ain Alsaeraa at Cairo, Egypt. They were identified as Penicillium purpurgonium (water), Aspergillus niger (water) and Aspergillus niger (soil). They tolerated wide range of pH from 1 to 11.Effect of incubation periods and temperatures on growth of isolated fungi was studied. Determination of total proteins, carbohydrates and lipids was carried out, significant increase was observed till pH6.  

 

 

4/35 A NEW STREPTOMYCES SPECIES PRODUCING A POTENT ANTIBACTERIAL AND ANTIFUNGAL ANTIBIOTIC

Fathi A. Mansour, El-Sayed E. Hafez*, Mohamed I. Abu Dobara**

and Ahmed M. El-Shobaky

Botany Department, Faculty of Science, Mansoura University, *Mubarek City for Scientific Research and **Botany Department, Damiatta Faculty of Science, Mansoura University

A new Streptomyces species (isolate no. 1), was identified according to molecular levels and its genetic sequence was determined and was found to produce a crimson red pigment which extracted from the culture filtrate of actinobacteria by n-butanol at neutral pH, chromatographical purified and obtained as crimson red powder. The bioautography of this purified substance ensure that it had a potent antimicrobial activity against bacteria, fungi and candida. The biological and physicochemical characteristics (UV, IR, NMR and Mass Spectrum of this purified antibiotic were investigated and accordingly the antibiotic pigment was identified as a novel antibiotic.

 

5/35 SOME PHYSIOLOGICAL PARAMETERS FOR ETHANOL PRODUCTION FROM BEET MOLASSES BY SACCHAROMYCES CEREVISIAE CAIM 13

Awatif A. Al-Judibi

Biology Department, Faculty of Science, King Abd-El Aziz, University KSA

This work was to evaluate the potentiality of crude beet molasses (CBM) for ethanol production by yeast fermentation and to examine some physiological parameters that might affect the fermentation efficiency. Ten strains of yeasts were screened for their production of ethanol from CBM; Saccharomyces cerevisiae CAIM 13 was the Strain with the best sugar-ethanol conversion (Fermentation efficiency 83.3%) and thus selected for further studies. The atomic absorption analysis of BM ash showed the presence (in order of concentration) of Na+, Ca2+, K+, Mg2+, Fe2+, Zn2+, Cu2+, P3+ and Mn2+. CBM was subjected to different treatments to reduce the elements content and to remove the muddy residue. The best results were obtained when CBM was treated with either H2SO4 or Ca3 (PO4)2 and heated to 90oC. Maximum ethanol productivity was attained after 48 h incubation at 30oC when mud-free, H2SO4-treated beet molasses (TBM) was used. The optimum pH for sugar conversion to ethanol was 5 and for yeast growth was 5.4. A sugar concentration of 10.86 g% gave optimum fermentation efficiency. The intermittent addition of TBM allowed better assimilation of sugars with the production of relatively high biomass yields and ethanol outputs. Maximum ethanol productivity was obtained at 1.08 g/litre urea. Fermentation activities were stimulated by either MgSO4.7H2O (0.3 g/litre) or FeSO4.7H2O (100 mg/litre), and inhibited on addition at ZnSO4.7H2O. Sugar assimilation and yeast growth were stimulated by either peptone corn steep solid, soybean oil or soybean flour. Ethanol productivity was retarded with either peptone or corn steep solid treatments.

 

 

6/35 CHANGES IN ENDOGENOUS POLYAMINES AND PROTEIN FRACTIONS IN SALT STRESSED WHEAT PLANT BY PUTRESCINE OR SPERMINE TREATMENTS

Fawzia Abo-Srea Ahmed Ebad; Samira Kamal Tahoon

and Abeer Esmaeil mohemmed

Botany and Microbiology Department, Faculty of Science (Girls Branch), Al-Azhar University, Cairo, Egypt.

A pot experiment was carried out to study the effect of polyamines pretreatments, putrescine (4&8 mM) or spermine (6 & 10 mM), on the content of endogenous polyamines and protein fractions in wheat plants grown under salinity stress (0.0, 8000, 10000 ppm NaCl). NaCl stress produced new protein bands, with molecular masses (mM) of 80, 40 and 16 KD. Also, new bands appeared in plants grown under putrescine or spermine treatments. The common new bands which appeared with polyamines application were Mm 80, 45, 40, 24, 16 and 14 KD, compared to their control. Content of putrescine decreased gradually with increasing NaCl level in irrigation water while, spermine and spermidine contents were generally increased under salinity stress.

 

 

7/35 AZOTOBACTER CHROOCOCCUM CYST IS A NEW PROMISE IN LIQUID BIOINOCULANTS UNDER EGYPTIAN CONDITIONS

Marwa S. Abdel-hamid, Ashrf F. Elbaz, Atef A. Ragab*, Hanafy A. Hamza and Khalil A. El Halafawy

Genetic Engineering and Biotechnology Research Institute, Minufyia University, Sadat City Branch, Egypt

*Central Laboratory of Organic Agriculture, Agricultural Research Center, Giza, Egypt

Species of Azotobacter are known to form heat and desiccation-resistance cysts that have a long life span. Encycsment in Azotobacter chroococcum can be induced by 0.2 % n-butanol, Ethanol and glucose as a sole carbon source. The morphology of vegetative and cyst cells were examined by electron microscopy and cyst’s stain.The resistance of cyst against different stress factors was investigated as the effect ofdesiccation-resistance. An experiment was carried out to study the survival of the vegetative and cyst cells of A. chroococcum in the liquid culture through long and different periods.The effect of A. chroococcum cyst based liquid bioinoculants under vegetative and cyst conditions on the growth of maize plants, were studied.

8/35 PHYSIOLOGICAL RESPONSES AND CORRELATES OF EUPHORBIA PLANTS GROWEN IN FIVE DIFFERENT SITES OF SOUTH SINAI.

Abd El-Monem M.A. Sharaf and Mohamed M. Moursy*

Botany Department, Faculty of Science (Boys Branch), Al-Azhar University,

Cairo, Egypt.

*Saint Katherine Protectorate, the Nature Conservation Sector (NCS), Egyptian Environmental Affairs Agency (EEAA).

The present investigation was carried out to study certain physiological adaptive responses of the Euphorbia sanctae-catharinae plants collected from five different locations in Saint Katherine Protectorate, south Sinai. Analysis was carried out through three stages of growth. A great diversity was recorded as regards the biochemical constituents of the plants among the different wadis in all stages of growth. The maximal value of total chlorophylls during the three stages was recorded in Wadi al-Tal‘a, while the minimal value was recorded in Wadi Gebal during stages A & B and in Wadi Zwateina during stage C.The maximal value of total soluble carbohydrates and proteins during stage A was recorded in Wadi al-Tal‘a, in Wadi al-shaqq during stage B and in wadi Gebal during stage C. Discriminant analysis of the obtained results revealed that the endogenous hormones play the more important role in the physiological correlates between the different plants during the three stages of growth. From the cluster analysis for protein profiles analyzed by gel electrophoresis, the highest similarity was found between plants from Farsh al-Rummana and Wadi al-Tal‘a (52% dissimilarity level), while the minimum similarity found between wadi al-shaqq and both of Farsh al-Rummana and Wadi al-Tal‘a (as one group) with 80% dissimilarity level.

 

9/35 EFFECT OF POLYAMINES PRETREATMENTS ON THE ACTIVITY OF ANTIOXIDANT ENZYMES IN SALT STRESSED WHEAT PLANTS

Fawzia Abo-Srea Ahmed Ebad; Eglal Mohammed Galal El-Dein

and Abeer Esmaeil Mohemmed

Botany and Microbiology Department, Faculty of Science (Girls Branch), Al-Azhar University, Cairo, Egypt.

A pot experiment was conducted to study the effect of polyamines pretreatments, putrescine (2, 4, 6, 8 & 10 mM) or spermine (2,4, 6,8 & 10 mM),on the activity of catalase, peroxidase, and polyphenl oxidase in salt stressed wheat plants. NaCl (0.0, 6000, 8000,10000 ppm) leads to gradually increase in the activity of catalase, peroxidase and polyphenol oxidase in wheat plants. On the other hand, activity of antioxidant enzymes was decreased by the application of different polyamines treatments, especially with higher levels, in plants which grown under salt stress (6000, 8000 and 10000 ppm NaCl)      

 

10/35 STUDY OF ACTION OF [1-(2-ETHYL, 6-HEPTYL) PHENOL] ON MORPHOLOGICAL FEATURES AND ULTRA STRUCTURES OF ASPERGILLUS FUMIGATUS AND CANDIDA ALBICANS

Amal, A.I. Mekawey

Researcher at the Regional Center for Mycology and Biotechnology- Al-Azhar University, Cairo, Egypt

1-(2-Ethyl, 6-Heptyl) Phenol (EHP), a biologically active compound formerly extracted by benzene from Cuminum cyminum (cumin) Egyptian seeds and exhibited great activity against a number of fungal and bacterial pathogens, also it had antitumor and antiviral potency, studying the effect of EHP on the morphological features and ultra structures of Aspergillus fumigatus and Candida albicans were investigated. Great changes (aggregation, distortion, elongation, swollen) of morphological features and alteration of ultra organelles measurements of tested fungal isolates were reported.


 

12/35 IMPACT OF MICROBIAL INOCULATION ON MAIZE (ZEA MAYS) PRODUCTIVITY UNDER DIFFERENT LEVELS OF POTASSIUM FERTILIZATION.

Hager, I. Tolba; Ebtsam, M. Morsy and El-Sayeda H.M. El-Badawy

Soils, Water and Environment Research Institute, ARC, Giza, Egypt

Two field experiments were carried out in a private farm at El-Ayaat, Giza governorate, Egypt, during summer seasons (2007 and 2008) to study the effect of inoculation with different microorganisms viz. Pseudomonas fluorescence, Pseudomonas putida, Bacillus circulans and Bacillus megaterium, and Trichoderma sp. on growth, yield and productivity of maize (Zea mays L.) hybrid 10 var. under three levels (0, 50 and 100%) of potassium sulphate (50% K2O). The obtained results exhibited the increase of total microbial count and the activity of dehydrogenase enzyme in the rhizosphere of maize plants at 50 and 80 days of planting with the plants inoculated with Ps. fluorescence and B. circulans along with 100% K. Moreover, plants gave the highest dry weight at the same level of potassium fertilizer compared to control. The maximum N, P and K contents were observed in the plants inoculated with Ps. fluorescence, B. megaterium and B. circulans, respectively. Therefore, the highest yield was obtained from the plants inoculated with Ps. fluorescence and B. circulans. Results also confirmed the positive impact of microbial inoculation with levels of potassium solubility on growth and maize yield as an economic crop.

 

13/35 RESPONSE OF TUBEROSE (POLIANTHES TUBEROSA, L.) PLANT TO DIFFERENT SOIL MIXTURES AND ETHEPHON UNDER RECLAIMED DESERT SOIL

Mohamed M. Abdel-Sattar, Samira S. Ahmed and Ali Nabih.

Horticulture Research Institute, Agriculture Research Center, Giza

The present study was conducted under full sun at the nursery of Hort. Res. Inst., Giza, Egypt during 2008/09 and 2009/10 seasons for elongating the flowering period of Polianthes tuberosa, L. plant to late summer and beginning of autumn, besides improving quantity and quality of the produced spikes and bulbs. So, bulbs of 7-8 cm circumference were planted in 25-cm-diameter clay pots filled with about 3 kg of one of the following media: clay (as a control), and a mixture of either sand + compost or sand + sewage sludge at 3:1, by volume. Ethephon solution was also sprayed on the foliage, 4 times with 3 weeks interval at the level of 0, 50, 100 and 200 ppm individually or in combination with the previous media. The obtained results indicated that a mixture of sand + compost gave the highest means of vegetative and flowering growth, earlier precocity and the longest period of flowering, as well as the highest number and best quality of the produced bulbs and bulblets compared to the other two media, while a mixture of sand + sewage sludge recorded the best content of N, P, K, amino acids and reduced and non-reduced sugars, plus reducing phenols content in the new bulbs. Spraying with ethephon had no positive effect on flowering precocity and all other vegetative and flowering traits, except for 100 ppm concentration that caused a slight increment in height of vegetative growth and bulb yield coupled with improving the new bulbs content of N, P, K, amino acids and reduced and non-reduced sugars, with reducing their content of phenols. The combining between planting in sand+compost mixture and spraying with ethephon at either 50 or 100 ppm level resulted the utmost high averages in all parameters measured. Hence, it could be recommended to add a compost to sandy soil (3 sand:1 compost, v/v) plus spraying with ethephon at either 50 or 100 ppm level to get early flowering and quantity and quality of flowers during a long period may reach late summer and early autumn, besides increasing number of bulbs and bulblets produced at the end of the season.
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