Vol. 24, September, 2009.

cialis sans ordonnance forum 1/24 EFFECT OF SODIUM BENZOATE AND SODIUM ACETATE ON PSEUDOMONAS AERUGINOSA AND ITS SUSCEPTIBILITY TO IMIPENEM

Ahmed A. Abdelaziz, Tarek El-Banna, Fatma Sonbol and Lamiea Al-Madboly

Department of Microbiology, Faculty of Pharmacy, Tanta University

The effect of 1 or 10 mM of benzoateoracetate sodium salts onPseudomonas aeruginosa and its susceptibility toimipenem was investigated. Some molecular mechanisms that explain these effects were also studied. Growth rates of P. aeruginosa were reduced in the presence of different concentrations of benzoate or acetate and this depends on the concentration of the weak acid used. NPN uptake measurements of acetate-treated cells of P. aeruginosa (POA1) were higher than those obtained by benzoate but these measurements were reduced after addition of Mg+2 ions in both cases. The ability of the P24 isolate to form biofilm was qualitatively diminished in a concentration -dependent manner when exposed to different concentrations of benzoate only. Addition of sodium benzoate to the bacterial growth media qualitatively and quantitatively reduced the production of pyocyanin by P24 isolate. When P. aeruginosa (PAO1), non-mucoid reference strain was grown in the presence of different concentrations of benzoate or acetate, only 10 mM acetate- treated culture was found to produce highly viscous solution. The MIC of imipenem was determined in absence and presence of 1, 10 or 16 mM of benzoate or acetate against 20 P. aeruginosa isolates and FIC values were calculated. Acetate /imipenem combination showed synergism with different percentages at different concentrations while benzoate/ imipenem combination showed synergism that changed into antagonism when 16mM benzoate was used. The late effect was confirmed by EOP test. The intensity of a major protein band of molecular weight 45 KDa, responsible for the entry of imipenem, was reduced in the presence of benzoate/ imipenem combination compared with that of benzoate alone at the same concentration (16 mM).

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cialis et norvir 2/24 EFFECT OF SALICYLATE AND ASCORBIC ACID ON KLEBSIELLA PNEUMONIAE AND ITS SUSCEPTIBILITY TO ANTIMICROBIALS

Tarek El-Banna, Ahmed A. Abdelaziz, Fatma Sonbol and Lamiea Al-Madboly

Department of Microbiology, Faculty of Pharmacy, Tanta University

The effect of salicylate or ascorbic acid on cialis accident vasculaire Klebsiella pneumoniae and its susceptibility to antimicrobials was investigated. Some molecular mechanisms that explain these effects were also studied. Both of salicylate and ascorbic acid reduced the growth rates of prix du levitra 20mg K. pneumoniae reference strain in a concentration-dependent manner except for 1mM ascorbic acid that increased the OD600 of the organism at the end of the growth curve. Morphological and ultrastructural changes were detected in salicylate-treated cells using light and electron microscopes and these changes include conversion to filamentous shape, reduction in capsular size and changes in ribosomal distribution within the cell. Profound NPN uptake was induced for viagra prix vidal K. pneumoniae cells by salicylate whereby the uptake was, to a large extent, reduced by addition of Mg+2 ions. Ascorbic acid was found to be a good permeabilizer but secondary to salicylate effect. While salicylate at concentrations 1 and 10 mM reduced the susceptibility of cialis bloqué en douane K. pneumoniae isolates to ampicillin and cephradine, the susceptibility to aminoglycosides and imipenem was potentiated. On the other hand, the susceptibility of the isolates to ciprofloxacin, tetracycline and ampicillin/sulbactam was dependent on the concentration of salicylate used. Moreover, ascorbic acid increased the susceptibility of the isolates to most antimicrobials. Salicylate increased the activity of K90 extracted β-lactamase while ascorbic acid reduced the enzymatic activity in a concentration-dependent manner. In addition, two β -lactamases of pIs 7.6 and 7.8 were detected in untreated and salicylate-treated samples while ascorbic acid-treated sample showed only one enzyme of pI 7.8. Plasmid DNA analysis revealed two plasmid bands (1.2 & 2 MDa) in control sample and both of them disappeared completely in all ascorbic acid-treated samples. Concentration-dependent reduction in the intensity of some major bands was detected in the outer membrane proteins of weak acid-treated isolate particularly in the presence of salicylate. The overall observed effects of salicylate or ascorbic acid on avis commission transparence levitra K. pneumoniae and its susceptibility to antimicrobials seem to be multifactorial.

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3/24 EFFECT OF CITRIC ACID ON E. COLI AND ITS SUSCEPTIBILITY TO ANTIMICROBIALS

Fatma I. Sonbol , Ahmed A. Abd El- Aziz, Tarek El-Banna, and Lamiea Al-Madboly

Department of Microbiology, Faculty of Pharmacy, Tanta University

The effect of 0.5 to 10 mM ofcitric acid onE. coliand its susceptibility toantimicrobialswas investigated. Some molecular mechanisms that explain these effects were also studied. A considerable growth retardation of the test organism was observed and this retardation reached its maximum in case of treatment with 10 mM citric acid concentration. NPN uptake measurements of citric acid-treated cells of E. coli ATCC 25922 were high but the uptake was, to a large extent, reduced by addition of excess Mg+2 ions. The susceptibility of E. coli isolates to most antimicrobial agents was reduced in the presence of 5mM citric acid particularly with ciprofloxacin, indicating antagonism and this was confirmed by agar double diffusion test that showed truncation near the junction of the inhibition zones of E. coli surrounding the tested antimicrobial agent and citric acid.Citric acid increased the production and activity of β-lactamase enzyme. In addition, two β -lactamases of pIs 7.6 and 7.8 were detected in isoelectric focusing electrophorogram in untreated sample while citric acid-treated sample showed the β-lactamase with pI 7.8 and a new one with pI 5.4.Plasmid DNA analysis revealed that citric acid had no effect on the plasmid profile of E58 isolate. Concentration-dependent reduction in the intensity of two major protein bands, detected in the OMPs of citric acid-treated E58 isolate, was recorded.

 

 

4/24 BIODEGRADATION OF CRUDE PETROLEUM OIL AND ENVIRONMENTAL POLLUTANTS BY CANDIDA TROPICALIS STRAIN AND APPLICATION OF PLACKETT-BURMAN DESIGN TO EVALUATE CULTURE CONDITIONS AFFECTING CRUDE OIL DEGRADATION.

Soha Faragand Nadia A. Soliman*

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt

*Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt.

 

Among group of environmental isolates, a yeast isolate, (designated as strain A) was isolated from motor oil polluted area of Abou-Qir gulf (Alexandria, Egypt) and selected due to its potential activity in petroleum degradation. Based on partial sequences of 18Sr RNA gene homology, the strain was 99% related to Candida tropicalis. The degradation potency of the isolate was tested in a natural sea water medium individually supplemented with petroleum oil, aliphatic, aromatic and some hydrocarbons derivatives as sole carbon and energy sources. A noticeable decrease in cellular diameter was illustrated in scanning electron microscope (SEM) examination upon growing the yeast isolate in natural sea water medium amended with petroleum oil as sole carbon source in comparison with complete medium. In addition, the isolate showed different biodegradation patterns with different hydrocarbons and the maximum degradation tendency towards naphthalene and phenol. Statistically-based experimental design was applied to evaluate the significance of factors on petroleum oil biodegradation by the yeast isolate. Eleven culture conditions were examined by implementing Plackett-Burman factorial design where aeration, NH4Cl and K2HPO4 had the most positive significance on oil degradation.

 

 

5/24 INSIGHTS ON THE MECHANISM OF AS(III) BIOREMEDIATION IN SEDIMENTARY ENVIRONMENTS

Eman Afkar

Department of Botany, College of Science, Beni-Suef University, Beni-Suef, Egypt.

The dissimilatory arsenate reductase (DAsR) from Sulfurospirillum barnesii has successfully purified without the hydrogenase subunit. It was purified with two successive steps of ion exchange chromatography (DEAE-Toyopearl) followed by a single step of gel filtration (Sephacryl S-300 gel). Although specific activity increased during the purification process, a significant amount of total activity was lost in the process. As compared to the activity of cell lysates (100%), a 20% loss of activity occurred in the membrane fractions, and a 43% loss of activity occurred in the CHAPS solublized fractions. Western blot analysis using polyclonal antibodies raised against the 30 kDa subunit were used to monitor the state of the complex in the different purification steps. The intact complex appeared in the whole cell lysates, then monomeric 30 kDa polypeptide first appeared in the membrane fraction and both the 30 and 22 kDa peptides were readily detected in the CHAPS solubilized fraction. Thus, the loss of activity could be explained by the disassociation of the complex. Enough of the DAsR was left intact to facilitate further study. A heteromultimeric complex of about 55 kDa it is comprised of monomers of 30 and 22 kDa. It can couple the oxidation of methyl viologen to the reduction of arsenate, selenate, and selenite, but not arsenite. This latter finding suggests that S. barnesii may use the same complex for growth on both arsenate and selenate reduction.

 

 

6/24 SEXUAL TRANSMISSION OF HEPATITIS –C VIRUS IN SPOUSESOF CHRONIC HEPATITIS-C PATIENTS

Mohamed Shees Ahmed, Almahdy M. Al-Atrouny, Ibrahim M. Al-Husseini, Mahmoud Hadad Hemida*

Microbiology and Immunology Department, and Internal Medicine Department*, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.

Hepatitis- C virus (HCV) has become a major public health problem in Egypt. There is controversy about the role of sexual transmission in HCV infection. In the present study, incidence of HCV in the spouses of chronic hepatitis –C (CHC) patients was assessed. Serum samples were collected from 75 CHC Patients and their spouses. They were tested for HCV antibodies using ELISA technique, and HCV-RNA using PCR techniqe. HCV genotype was determined in +ve spouses and their partners using PCR technique. Spouses of 25 normal healthy persons were taken as a control. HCV antibodies were detected in 18(24%) spouses, of whom 17(22.6%) were also +ve for HCV-RNA in the patients group, and the genotype was identical (genotype 4) in all +ve spouses .While in the control group HCV antibodies and HCV-RNA were found in only one person (4%) (p<0.05). HCV seropositivity in the spouses was related to duration of marriage .No difference was found between female to male or male to female transmission. In conclusion, spouses of CHC Patients have an increased risk for acquiring HCV. They should be screened routinely for markers of HCV infection.

 

 

7/24 COMPARATIVE STUDIES ON KERATINASE PRODUCTION BY BACILLUS PUMILUS AND BACILLUS LICHENIFORMIS USING SOLID STATE FERMENTATION

Mona A. Esawy; Walaa Abd El-Salam; Ghada E.A. Awad; Ebtesam N. Hosseny*; Zeinab A. El-Awamry* and Ahmed E. El-Diwany

Department of Chemistry of Microbial and Natural Products Chemistry; National Research Centre, Dokki, Cairo, Egypt.

*Department of Botany and Microbiology, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.

Different bacterial isolates from Red sea coast and shallow water in Alexandria were screened for their ability to produce keratinase that catalyzes the degradation of animal horns. The most potent producers were identified as Bacillus puimlus and Bacillus licheniformis on the basis of their morphological and biochemical characteristics (API). The two organisms were able to grow on solid state fermentation in presence of different types of keratinaceous and non-keratinaceous materials. Maximum formation of keratinase occurred when B. puimlus was grown on sugar cane bagasses, while that of B. licheniformis was obtained in presence of banana skin. The results showed that the maximal production of keratinase by both organisms was obtained after 48 hr incubation at pH 7 and 30ºC. The optimal moisture content for the enzyme formation by B. puimlus was 200% (w/v), whereas that for B. licheniformis enzyme was 250 % (w/v). The effect of different metal salts and carbon sources on keratinase formation was also investigated.

 

8/24 The promotive effect of algae and Rhizobium leguminosarum on arbuscular mycorrhizal fungi activity and their impact on faba bean plant

Manal A.H. El Gamal, Osama N. Massoud and Olfat M.A. Salem*

Soils, Water & Environ. Res. Inst., Agric. Res. Center, Giza, Egypt

*Botany and Microbiol. Dept., Fac. Science, Helwan University

The stimulatory effect of two algal species namely Nostoc muscorum (liquid culture) and Ulva lactuca (air dried powder) on AM-fungi development and their impact on the growth of faba bean in the presence of Rhizobium leguminosarum under low level of N and P fertilizers (50 and 33.3 %, respectively) in pot experiment that performed in greenhouse was investigated. Both algal species were applied to the plant at three intervals (after 2, 4 and 6 weeks from sowing) in two rates, i.e., 100 and 200 ml/pot for N. muscorum and 0.25 and 0.50 g/pot for U. lactuca. As compared to control, there were significant differences observed between the treatments due to the application of algae, mycorrhiza and Rhizobium, either in single or in a mixture, on the number and dry weight of nodulation, shoot and root length and dry weight, branches and pods number, 100-seed weight, nutritional value of the plants, in addition to the biological activity in rhizosphere region through the determination of soil dehydrogenase and nodules nitrogenase enzymes as well as the root infection percent of mycorrhizal fungi and leaves pigment content. In the majority of cases, the application of high rates of both algae in combination with mycorrhiza and Rhizobium resulted in increases in the root length and dry weight, nodule number, also shoot length and dry weight, weight of 100-seed, shoot NPK and seed protein as compared to the other tested treatments. Moreover, results indicated the possibility of reducing N and P fertilizers by 50 and 66.6 % under greenhouse condition, respectively, of the recommended dose without affecting faba bean yield by the combination between algae, mycorrhiza and Rhizobium. So, more attention must be paid due to the utilization of bio-inoculants to crops to decrease costs and ecology pollutant.

 

 

9/24 PREVALENCE RATE OF DERMATOPHYTOSIS, RISK FACTORS AND THE DERMATOPHYTES SPECIES ISOLATED FROM SCHOOLCHILDREN IN ADEN, YEMEN

Dheya A.H. Sharaf Al-Danani, Hassan A. Al-Shamahy

and Amat-Allatif Y. Abu Taleb*

Department of Medical Microbiology, *Department of Community Medicine, Faculty of Medicine and Health Sciences. Sanaa University,Yemen.

Seventy two thousands five hundred and thirty three students (Boys and girls) selected from thirty primary public children schools in Aden city, Yemen they were clinically examined for dermatophytosis. This study was conducted to determine the prevalence rate of dermatophytosis in primary public children schools in Aden city, Yemen. And to determine the different dermatophytes species isolated among students. Finally to identify the risk factors that aids in the infection by the dermatophytes. The results obtained from this study, that only 110/72533 students were clinically diagnosed as infecting by dermatophytosis. The prevalence rate of dermatophytosis among students in the thirty primary public children schools was 0.4 %. Only 45/110 samples were positive by the direct examination (KOH test) but 25/110 samples were positive by culture. The twenty five positive cultures yielded eleven dermatophytes species; eight of them are anthropophilic species (T.mentagrophytes, T.ferrugineum, T.tonsurans, T.rubrum, M.auidonii, T.soudanense, T.violaceum and E.floccosum) two are zoophilic species (M. canis and T.verrucosum) and the last one is a geophilic dermatophyte (M.gypsium). Among the different clinical variants of dermatophytosis (Tineas) found among students; tinea manuum constituted 60%, followed by t. pedis 20%, t. capitis 12% and lastly t. corporis 8%. Male students were infected by dermatophytosis higher than female students, and therewere significant risk factorsof infecting by dermatophytes in both; contact with animals and shared towels (p- value<0.05). From this study it can be concluded that, first, the prevalence rate of dermatophytosis among primary public children schools in Aden city was 0.4%. Second, there were eleven dermatopytes species isolated among the infected students from the three genera; Trichophyton, Microsporum and Epidermophyton. Third, there was a strong correlation between the ecology of dermatophytes species isolated from the infected students (anthropophilic, zoophilic and geophilic) and the risk factors found among these students in both; shared towels and contact with animals.

 

 

10/24 PENICILLIN PRODUCTION BY IMMOBILIZED CELLSOF PENICILLIUM CHRYSOGENUM NRRL 824

Mona I. Shaaban, El-Sayed E. Habib, Mohamed A. El-Sokkary, Wael A. El-Naggar

Department of Microbiology, Faculty of Pharmacy, Mansoura University

The production of penicillin by free and immobilized cells of Penicillium chrysogenum was investigated. The cells were immobilized using, synthetic sponge, cotton balls, loofa pieces as well as calcium alginate. The maximum amount of penicillin reached up 128±10.37 µg/ml was obtained, using loofa sponge as a carrier as compared to free cells production (19.4±2.05 µg/ml). In addition to 31.9±4.5 µg/ml and 48.9±7.2 µg/ml of penicillin obtained by using the cells immobilized by sponge cubes and cotton balls respectively. However, the use of calcium alginate beads produced lower yield (6±0.53 µg/ml) than obtained by free cells but different numbers of used loofa pieces, sponge cubes and cotton balls greatly influenced the production of penicillin. The highest antibiotic yield was obtained by using 16 equally sized sponge cubes, 20 cotton balls and 5 loofa pieces (31.9±4.5, 48.9±7.2, and 136.9±10.37 µg/mlrespectively). During repeated batch fermentation process, loofa-immobilized cells exhibited higher penicillin production (340µg/ml) at the 4th fermentation cycle than other immobilized cells under similar experimental conditions. Therefore, immobilization of P. chrysogenum cells using loofa can be successfully used to increase   penicillin production more than other carriers.

 

 

11/24 MULTIDRUG RESISTANT OF STAPHYLOCOCCUS EPIDERMIDIS IN NEONATAL INTENSIVE CARE UNITS

Salwa S. Afifi, Wafaa El Taibe* and Mohamed Salah**

Microbiology & Immunology Department, Faculty of Pharmacy (Girls and Boys**),

Al-Azhar University**, Miser International University (MIU)*

Multiple drug resistant Staphylococcus epidermidis have been clinically isolated from the Neonatal Intensive Care Unit (NICU) of EL-Sahel Educational Hospital. These clinical isolates were subjected to identification, antibiotic susceptibility testing, phenotyping by antibiogram output data, and also to genotyping by randomly amplified polymorphic DNA (RAPD) analysis using different primers and plasmid profile as well as detection of mec A gene. Susceptibility testing demonstrated that all the S. epidermidis isolates were multidrug resistant (MDR) and exhibited resistance to oxacillin (MIC ≥ 2µg/ml). The majority of clinical isolates showed resistance to other tested β-lactams, gentamicin, erythromycin and azithromycin. S. epidermidis clinical isolates retained susceptibility to the glycopeptide vanomycin, fluoroquinolones, and to other classes of antibiotics. Six antibiotypes were obtained by antibiogram from the 29 clinical isolates of S. epidermidis. Plasmid typing of the 29 S. epidermidis clinical isolates yielded 13 different plasmid patterns (A-M), each with 1-5 bands with molecular weight between ≤ 1.5 and 0.5 Kb. Plasmid pattern F was the predominant pattern followed by pattern E then patterns A, B, C and D (2 out of 29 isolates for each type). The remaining patterns (G through M) were unique for single isolate. However, seven isolates did not yield any bands and were assigned as pattern N. For mec A gene detection, all isolates gave a 154-bp amplified DNA fragment and was detected on a 2 % agarose gel. The suitability of RAPD for the detection of differences between multidrug resistant S. epidermidis isolates was evaluated. Genomic DNAs from clinical isolates of S. epidermidis were examined by PCR-based RAPD fingerprinting analysis using five oligonucleotides primers. Only 2 primers out of five tested primers gave the best results. Primer 5 typed the isolates into 17 different patterns while primer 3 typed all isolates into 4 different patterns. Five S. epidermidis isolates had common RAPD types, using primer combination, namely isolates number 31, 29, 47, 41 and 85. A common RAPD type among S. epidermidis isolates point to the dissemination of a S. epidermidis clone in the NICU environment. In conclusion, the results showed that the PCR-based RAPD fingerprinting method distinguishes genetically different strains of multidrug resistant of S. epidermidis and may be valuable for monitoring transmission of this pathogen.

 

 

12/24 RATIONALIZATION OF POTASSIUM FERTILIZER APPLIED FOR SUGAR BEET GROWN UNDER DIFFERENT IRRIGATION SYSTEMS BY USING BIOFERTILIZATION

Hager I. Tolba, Ahmed M. Awad*and Hanaa A. Abo-Kora

Agricultural Microbiology Department, Soils, Water & Environment Research Institute (SWERI), Res. Station, ARC. *Soil Fertility and Plant Nutrition Dept. (SWERI), Nubaria Agric. Res. Station, ARC.

A field experiment was carried out at El-Nubariea Research Station, Alexandria Governorate, Egypt, during winter season (2006/2007) to study the effect of Bacillus circulans (silicate bacteria) and feldspar on growth parameters, minerals contact, yield components and sugar content % of sugar beet (Beta vulgaris L) by using two surface irrigation types (rows and terraces). Results show that : treatment of B. circulans plus recommended dose of feldspar (T4) gave the highest shoot and root dry weights, K and P content % and yield (47 ton/ feddan) with rows, whereas, the sugar content % in terraces was significantly higher than it in rows, since terraces had recorded 18.5%. Regarding to total bacterial count and dehydrogenase activity, the best results was due to with (T4), where the highest counts were recorded with rows. While, dehydrogenase activity was higher in terraces than it in rows. So, the present study supports the new trend of using biofertilizers and natural K fertilizer (feldspar) as a beneficial cheap source of fertilization for sustainable agriculture beside using suitable irrigation system to achieve best crop yield.

 

13/24 FUNGAL CONTAMINATION AND MYCOTOXIN DETECTION OF MARKETED ORAL PRODUCTS OF HERBAL ORIGIN

Mounir M. Salem Bekhet and Fars K. Alanazi*

Microbiology and Immunology Department, Faculty of Pharmacy, Alazhar University, Cairo, Egypt.

*Kayyali Chair for Pharmaceutical Industries, Department of Pharmaceutics, College of Pharmacy, King Saud University, Saudi Arabia

Contamination of non-sterile pharmaceuticals (NSP) by Aspergillus species and their toxic metabolites is a serious problem as they have adverse health effects. The goal of this study is to evaluate the microbial quality of different brands of NSP oral preparations from herbal origin marketed in King Saudi Arabia (KSA). They were randomly analyzed for the presence of fungal aflatoxigenic contaminants and rapid assessment of aflatoxigenic fungi in tested samples using PCR. The PCR was performed using two different sets of primers specifically targeted to aflR and omt genes of aflatoxin biosynthesis pathway. Most of the fungi belonging to Asp. flavus and Asp. parasiticus groups reacted positively with the primers resulting in expected size amplicons of 796 bp for aflR and 404 bp for omt. Among 96 samples screened for aflatoxin production, 11 Asp. flavus strains were isolated. nine samples were contaminated with aflatoxin. It is concluded that genes involved in the aflatoxin biosynthetic pathway may form the basis for an accurate, sensitive, and specific detection system, using PCR, for aflatoxigenic strains in NSP preparations. Also, the results of this study highlight the necessity for strict control of NSP products.

 

 

14/244 AN OUTBREAK OF KLEBSIELLA PNEUMONIAE PRODUCING CTX-M-15 FROM INTENSIVE CARE UNIT,

RIYADH, SAUDI ARABIA

Mohamed Salah Ali and Mohamed Hamed M. Al-agamy*

Microbiology Department, Riyadh College of Dentistry and Pharmacy & *Microbiology Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

Seventy five Klebsiella pneumoniae strains were isolated in 2008 from hospitalized patientsduring an outbreak in intensive care unit, Al-Ashemesy hospital, Riyadh, Saudi Arabia. These isolates were resistant to multiple antibiotics, and phenotypically produce extended spectrum β-lactamase. Genotypic characterization of extended spectrum β-lactamase producing Klebsiella pneumoniae showed that all isolates carry blaTEM-1, blaSHV-1, and blaCTX-M-15 genes. Conjugation experiments by broth matting assay revealed that the resistance to third generation cephalosporins was encoded on transferable plasmid. This outbreak was due to CTX-M-15-producing Klebsiella pneumoniae isolates and this study indicated that blaCTX-15 is the dominant extended spectrum β-lactamase in Saudi Arabia

 

 


15/24 PRODUCTION, PARTIAL PURIFICATION AND CHARACTERIZATION OF AN INDUCIBLE EXTRACELLULAR LIPASE FROM PENICILLIUM MELINII

Adel A. El-Mehlawy, Hassan A. El-Minofi*, Hala M. Rifaat**, F. M. El-Beih and Samah A. Zaki

Microbiology Department, Faculty of Science, Ain shams University. *Chemistry of Natural Products Department, National Research Centre. **Microbial Chemistry Department, National Research Centre.

A total number of 64 fungal isolates isolated from soil rhizosphere of bean and cotton plant were subjected to lipase assay by using three different media (A, B and C). Penicillium melinii isolated from rhizosphere of cotton plant produced an inducible extracellular lipase in fermentation medium (C). Several factors including inoculum size, pH, incubation period, incubation temperature, carbon and nitrogen sources were examined in order to identify the optimal conditions of lipase production. The enzyme was partially purified by ammonium sulfate precipitation. The lipase was stable in the pH range of 4 to 10 and at temperature range of 20 to 90ºC for 30 minutes.

 

 

16/24 RAPID DIAGNOSIS OF NEONATAL BACTEREMIAUSING POLYMERASE CHAIN REACTION

Amany El Sharif and Raghdaa Ali*

Departments of Microbiology & Immunology, Faculty of Pharmacy (girls), Al Azhar University, Nasr City, Cairo, Egypt.

*Pediatrics and Neonatology, Ahmad Maher Teaching Hosp., - Cairo

Infections in the neonate are the most important causes of mortality and hospitalizations in the neonatal practice. In the present study, 180 neonates with criteria for probable sepsis admitted to neonatal intensive care unit in Ahmad Maher Teaching hospital were investigated for evidence of sepsis. The investigation protocol included sepsis screen, blood culture and molecular analysis by polymerase chain reaction (PCR) for bacterial DNA component encoding 16S rRNA in all cases. We compared the results of PCR with blood culture and other markers of sepsis screen. Blood cultures were positive in 80 (44.4%) cases with sensitivity of 91.9%. PCR was positive in 87(48.3%) cases (49 of them are with positive blood culture, CRP and buffy coat test). The sensitivity of PCR was 100% and specificity was 93%. The negative predictive value was 100%. The study concluded that although blood culture is a reliable method for diagnosis of neonatal sepsis, the universal bacterial primer PCR is a useful test and superior to blood culture for early diagnosis of sepsis in neonates. Consequently unnecessary treatment with antibiotics could be avoided.

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